agilent hplc infinity ii product introduction - labfarmasi.com · • it can be clustered with an...

Agilent HPLC Infinity II

Product Introduction

April 23, 2019

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Vision Scientific

2019 CLICK HERE FOR CONTACT US

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https://labfarmasi.com/hubungi-kami?source=HPLCagilentSurabaya

Agilent SupportBusiness Development Manager : Etin Diah P.hd

Technical Support Application Engineer

Jakarta

Doan Rizki (12 years experience in pharmaceutical industry)

Wahyu Eko Saputro Cathy (12 years experience in pharmaceutical industry)

Ifan Liza Hidayani S.Si Apt (8 years experience in BaBe Lab)

Risanto Reta Adji

Muhammad Fathurrachman

Fadillah

Randy

Surabaya

Gunarso

Jawa Tengah

Budi Mega Prasetyo

Bandung

Sugiyoto

Technical Support : Qualification (IQOQPV) Service and MaintenanceApplication Support : Method Development, Column Selection and Sample prep

1220Infinity II LC

400 or 600 bar

Affordable Efficiency

The Agilent 1220 Infinity II LC is an affordable, high-quality integrated LC system, putting you on the fast track to efficiency.

1290Infinity II LC

1300 bar

The Benchmark in Efficiency

The Agilent 1290 Infinity II LC embodies the next generation of LC, giving you the ultra-high performance to achieve maximum efficiency.

Agilent InfinityLab LC SeriesModern HPLC for Every Lab – Unlimited Flexibility

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1260Infinity II LC

400 or 600 bar

Everyday Efficiencyin Every Way

The Agilent 1260 Infinity II LC is the trusted platform with the broadest instrument choice, taking you to the next level of efficiency.

1260Infinity II Prime LC

800 bar

Agilent’s most convenient LCfor routine analysis

The1260 Infinity II Prime LC is the most capable and comfortable LC within the 1260 Infinity II LC portfolio.

LC Portofolio

4.6mm columns

3.0mm columns

2.1mm columns

1.0mm columns

Performance

Prime LC Quat 800 bar

Pressure

1290 Quat/Bin

1300 bar

Acquity ARC

Quat 655 bar

1220 Gradient

400 bar

1260 Quat

400 bar

Acquity H-Class

Quat 1034 barAcquity I-Class

Binary 1241

bar

1220 Gradient

600 bar

1260

Quat/Bin 600

bar

AlIiance

Quat 345

bar

5 um columns

3 um columns

< 2 um columns

i-Prominence

Quat 440 bar

i-Nexera Quat

660 bar

Nexera x2

Quat/Bin 1300 bar

HPLC UHPLC

1220 Infinity LC. Agilent Restricted

Isocratic or Gradient Pump (2-Channel):

• flow range: 0.2 - 10 mL/min• low pressure mixing• integrated degasser • flow precision 0.07 % RSD

Manual Injector or Autosampler:•600 bar• injection range: 0.1-100 µL•100 x 2 mL vials or 30 x 6 mL

vials• injection cycle time 50 s•carryover: < 0.05%

Column Oven:•up to 80°C•one column, max. 250 mm

VWD :•80 Hz data rate•wavelength range:190 - 600 nm

(VWD)

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Agilent 1220 Infinity II LCProduct Information

Agilent 1260 Infinity IIOverview

Multicolumn Thermostat4-110 oC

400 or 600 bar Pump

Multisampler432 Vials 2 mL

Sample cooler 4-40oC

Vialsampler 132 Vial 2mL Sample Cooler 4-40 oC

Integrated Column CompartmentUp to 80 oC

User interface – the InfinityLab LC Companion

Many Detectors

Agilent Prime LC/ 1290 Overview

Multicolumn Thermostat4-110 oC

800 or 1300 bar Pump1290 Infinity II pump technologyISET (Intelligent System Emulation Technology)

Multisampler432 Vials 2 mL

Sample cooler 4-40oC

Vialsampler 132 Vial 2mL Sample Cooler 4-40 oC

Integrated Column CompartmentUp to 80 oC

User interface – the InfinityLab LC Companion

Many Detectors

April 23, 2019

Confidentiality Label

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Quatenary Pump :

• 400 bar or 600 bar

• flow : 0.001 - 10 mL/min

• low pressure mixing

• integrated degasser

• 4 Solvent

Agilent Infinity II LC – 1260 Quatenary Pump

Infinity II Flexible Pump (Prime and 1290)

1260 Infinity II Prime LC

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Built-in Multipurpose Valve

Flexible Pump:

• 800 bar (prime) or 1300 bar (1290)

• flow : 0.001 - 10 mL/min

• low pressure mixing

• integrated degasser

• 4 Solvent

Infinity II Flexible Pump

April 23, 2019

Agilent Confidential

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Mixing starts here

BlendAssist – A simple tool for online dilution of modifiers and gradient setup

BlendAssist for automatic solvent blending


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H2O

+ 0.01%

TFA

MeCN

+ 0.01%

TFA

H2O

+ 0.05%

TFA

MeCN

+ 0.05%

TFA

H2O

+ 0.1%

TFA

MeCN

+ 0.1%

TFA

OR OR

OR...OR...OR....

Time consuming, costly & error-prone !



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H2O

MeCN

H2O

+ 1% TFA

MeCN

+ 1% TFA

H2O

+ 0.2% TFA

MeCN

+ 0.18% TFA

Type in desired

concentration!Run

gradient!

Pure solvent + concentrated stock solution = any concentration you need!




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Impurity B

Example 1: Different TFA concentrations

min0 1 2 3 4 5 6 7 8

mAU

0

100

200

300

400

0.05 M buffer

0.025 M buffer

0.01 M buffer

Procainamide

Acetyl-Procainamide

Disopyramide

Quinidine

Example 2: Different Phosphate buffer concentrations


0.02% TFA

0.20% TFA

0.10% TFA

0.05% TFA


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*

1290 Infinity II1300 bar - G7104A

Flexible Pump

1290 Infinity II1300 bar - G7120AHigh Speed Pump

Long Life Technology


1260 Infinity II800 bar - G7104C

Flexible Pump


Intelligent System Emulation Technology

Keep your method and get the same results on the

Infinity II Flexible pump LC

with ISET !!

Intelligent System Emulation Technology

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Time (min) %A %B

0 100 0

0.999 100 0

1 0 100

3 0 100

Single step gradient

Solvent A Solvent B

Commands from SW, programmed gradient

What the column head sees

0min 1min 2min 3min

Delay volume Mixing behavior

How it works - Example

Select vendor

Select system

Select sampleloop

Infinity II Flexible Pump method transfer from Waters Intelligent System Emulation Technology


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Infinity II Column Compartment

ICC MCT

Agilent Infinity II ICC (Integrated Column Compartment)

• Temperature Range : 5 °C above ambient to 80 °C• The integrated column compartment is a heatable (no

cooling) • It exists in different versions with volumes of 3 μl and

6 μl.• A tag reader for two columns is optionally available.• It can be clustered with an external valve for column

switching

Easy access column compartment with InfinityLab Quick Connect fittings and new column identification kit

Agilent Infinity II MCT (Multicolumn Thermostat)

• Temperature Range : 4 °C to 110 °C, (minimum 20 °C below ambient)

• The integrated column compartment is a heatable and cooling

• It exists in different versions with volumes of 3 μl and 6 μl.

• A tag reader for four columns is optionally available.

• It can be clustered with an internal valve for column switching


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Infinity II Multisampler and Vialsampler

Multisampler Vialsampler

Agilent Infinity II LC - VialsamplerInfinity II Vialsampler

+ Vials 2 mL (66 vials/tray, total 132 vials)+ Integrated column compartment+ Flexibility, great options

+ multi wash (2 solvents)+ Sample cooler 4 °C to 40 °C

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Infinity II Multisampler+ Vials 2 mL (54 vials/tray, 108 vials/rack)+ Well plates available+ High capacity up to 16 WPS (6144 samples)+ Extremely fast (needle to vial)+ High flexibility, great options, great upsell potential

+ dual needle or multi wash (3 solvents)+ Sample cooler 4 °C to 40 °C

+ Highest Capacity per benchspace (2 mL vials maks 432 vials)

96 Well plates, 0.5 ml, polypropylene,

Agilent Infinity II MultiSamplerProduct Information

Drawer

• Holds 2 individual sample containers• LED status indicator

Hydraulic block

Dual-needle park stationsReference vials

Sample Hotel

• Fully scalable• Up to 8 drawers• Flexible for vials & wellplate formats• Convenient access

• Flush pumps• Solvent selections• Metering devices• Injection valve

Agilent Infinity II - Multisampler

96 Well plates, 0.5 ml, polypropylene,

• The dual-needle setup of the 1290 Infinity II Multisamplerenhances flexibility by providing two injectors in a single instrument. You can optimize one path for large volume injections and the other for low delay volumes.

20µl

500µl

Agilent Infinity II - Multisampler

Agilent Infinity II Sample Cooler

SpecificationAmbient Operating Temp. 4-40°C (39,2 - 131°F)Temp. range from 4°C to ambientTemp. settable from 4 – 40°C in 1° C incrementsTemp. Stability +/- 1 °CTemp. Accuracy +/- 2°C

Agilent Infinity II Sample Cooler

1260 Infinity II Variable Wavelength Detector High Data rate Dual wavelength detection Cost effective detection

1260 Infinity II ELSD Lower limit of detection Fewer assays

1260 Infinity II DAD Lower limit of detection Fewer assays (HDR) Maximum method flexibility

1260 Infinity II MWD Lower limit of detection 5 Wavelength detection

Agilent Infinity II LC Detector

1260 Infinity II RID 74 HZ data rate Automatic solvent recycle Automatic Purge

1260 Infinity II FLD (G71721B) On-Line scanning capabilities Spectral Analysis 148 Hz Data rate

Deuterium Lamp

Max-Light Cartridge Cell

Programmableor fixed slit

1024 elementdiode-array

GratingMirror

Optofluidic Waveguides: Max-Light Flow Cells

1260 / 1290 Infinity II DAD

Agilent 1260 / 1290 Infinity II LCDiode Array UV Detectors (DAD)

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10 mm pathlengthsmall geom. cell volume (13 µL)

high light transmission

60 mm pathlength4 µL σV dispersion volume

Optofluidic waveguides (total internal reflection)

> 10-X Higher Sensitivity 1260/1290 Infinity DAD compare to 1200 Series DAD SL

1260/1290 DAD60 mm

1200 DAD SL 10mm

Height [mAU] 28.876 4.938

Noise [mAU] 0.0098 0.0190

Signal/ noise 2944 259

Increase 11.4 x

Columns: 150 x 4.6mm Zorbax SB C18, 5µmSample: Anthracene: 835 pg/µLMobile phase: A: Water, B: Acetonitrile Elution: isocratic 80 % BInjection volume: 5 µLFlow: 1.5 mL/minDAD: 251/4nm, Ref= 450/80nm,

2.5Hz, slit width 4nm

min0 1 2 3 4 5

mAU

0

5

10

15

20

25

30

1200 Series DAD / DAD SL

1260/1290 Infinity DAD

Column Options

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Option PhaseID

(mm)Length (mm)

Particle(µm)

Part Number

#096 EC-C18 3.0 100 2.7 695575-302T#097 EC-C18 3.0 150 2.7 693575-302T#098 EC C18 2.1 50 1.9 699675-902T

#093 EC-C18 4.6 100 2.7 695975-902T#094 EC-C18 3.0 150 2.7 693975-302T#095 EC C18 3.0 50 2.7 699975-302T

#090 EC-C18 4.6 100 4 695970-902T#091 EC-C18 4.6 50 2.7 699975-902T#092 EC-C18 4.6 150 4 693970-902T

Isocratic (G7110B)Quaternary (G7111B)Binary (G7112B)

Quaternary VL (G7111A)

400

bar

Every pump is shipped with a packed column (one pre-written tag included).One column must be chosen (no multiple choices)!

600

bar

Flexible (G7104C)

80

0 b

ar

1000 bar

1000 bar

NEW

Perfect fit for your specific solutions

Scaling Performance – 1260 Quat for UHPLC Performance in EVERY LAB!

Resolution optimized3.0x100mm, 1.8µmResolution peak 5 = 7.16Run time 7 min0.9mL/min, 460bar

Speed and resolution optimized3.0x50mm, 1.8µmResolution (peak 5) = 4.79Run time 1.1min2.4ml/min, 540bar

Conventional4.6x150, 5µmResolution peak 5 = 4.20Run time 11 min

min0 2 4 6 8 10

min0 2 4 6 8 10

min0 2 4 6 8 10

10x productivity increase and improved data quality!

Agilent Infinity II

Feature

April 23, 2019

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Vision Scientific

2019

Feature – Injector Program

April 23, 2019


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• Dengan injector program, user dapat menggunakan sample preparasididalam alat, dan juga untuk analisa asam amino menggunakan proses derivatisasi secara automatisasi dapat memberikan hasil yang optimal dan menghilangkan error yang dapat terjadi saat preparasi

Feature – Leak sensor• Leak Sensor

• Leak Sensor di setiap module (pump, autosampler, column compartment, detector), darifungsi leak sensor ini akansegera menghentikaninstrument yang sedangberjalan dan juga akanmenginformasikan telah terjadikebocoran kepada user

• Bottle Fillings

• Fitur software dimana kondisipump akan off jika solvent habis, sehingga akanmenghindarkan kerusakanpiston dan seal pada pompa

Prevent analysis if level falls below and Turn pump off if running out of solvent

Lab Advisor Basic Highlights

Tests

Calibrations

Tools

Firmware Update

Logs and Results

Fast Connect

System Overview

System Report

April 23, 2019

Lab Advisor B.02.06 Sales Training - Agilent Confidential

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Dengan fitur ini, user akan lebihdimudahkan dalam menanganitroubleshooting sebagaipenanganan awal dalam masalahyang dihadapi

Advanced EMF

Lab Advisor BasicKeep Track of Instrument Wear

April 23, 2019

Lab Advisor B.02.06 Sales Training - Agilent Confidential

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Fine tune EMF values to reflect your use of theinstument and get an alert well before instrument efficiencydegrades.

InfinityLab LC CompanionOverview

A modern replacement of the Agilent Instant Pilot (G4208A) for LCs as a general local remotecontroller is required. The new product is a firmware (FW) web-service, providing remote control capability of PL29 instrumentation on platform independent client hardware (HW)

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A new state-of-the-art local user interface

Agilent Dissolution

Product Portofolio

April 23, 2019

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Vision Scientific

2019

Dissolution Product PortfolioOverview

• Dosage Forms We Test

• USPApparatus 1, 2, 5, 6 & Intrinsic

• Dissolution Accessories

• Qualification Instrumentation

• USP Apparatus 3 and 7

• Small Volume Dissolution

• On Line Analysis

• Dissolution Workstation Software

Dissolution ProductPortfolioWhat do we test?

• Dissolution is not just about orally ingestedproducts.

• We test tablets, capsules, powders, ointments, creams, gels, osmotic pumps, transdermal patches, implants, medicated contact lens, stents, coated beads, catheters, wound care products, etc.

Drug Eluting StentContact LensPacemaker Lead Ophthalmic Implant

Tablets/capsules

Suspensions

Ointments

Transdermal patches

Dissolution Product PortfolioDissolution Apparatus

Depending on the nature of the dosage form, the USPrecommends using specific apparatus for testing:

USP Dissolution Apparatus 1, 2, 5, 6:1 – Rotating Basket2 – Paddle5 – Paddle over Disk 6 – Rotating Cylinder

USP Dissolution Apparatus 3, 7:3 – Reciprocating Cylinder7 – Reciprocating Holder

Dissolution Product Portfolio708-DS Dissolution Apparatus – for USP Apparatus 1/2/5/6

708-DS Dissolution Apparatus

Dissolution Product Portfolio708-DS Dissolution Apparatus – for USP Apparatus 1/2/5/6

Key Features:

Flexibility – 1-Liter standard configuration; can also support 100/200 mL or 2-Liter volumes

Automated – add sampling, temperature, or dosage introduction to improve repeatability

Scalable – add key features at any time to improve testing at any level

Manual

Semi-Automated

Online

Dissolution Product PortfolioAccessories for 708-DS (USP Apparatus 1, 2, 5, 6)

USP 1: Rotating Baskets USP 2: Paddles

USP 5: Paddle over Disk (Transdermal patches)

USP 6: Rotating Cylinder (Transdermal patches)

Dissolution Product PortfolioOther Accessories for 708-DS

Enhancer Cells <1724> (Gels, creams, ointments) Intrinsic Dissolution

Sinkers Stationary Basket“Mini” Accessories

Dissolution Product PortfolioDissolution Qualification Tool(s)

280-DS MechanicalQualification System (MQS)

• RPM• Shaft and Basket Rim Wobble• Shaft and Vessel Verticality• Vessel Table Level• Vibration• Temperature• Vessel Centering• Paddle/Basket Depth

Dissolution Product Portfolio280-DS Mechanical Qualification System (280-DS)

280 DS MQS benefits:• Time Savings (30 minutes vs 2 days)

• Data that helps identify instrument problems

• Easily track and monitor variations over time

• No guesswork or manually documenting values

• No standards or tablets to purchase

• Daily tool for failure investigations

• Ease of use allows for shorter calibration intervals –narrows the scope of potential failure investigations

Dissolution Product PortfolioDissolution Apparatus 3, 7

BIO-DIS Reciprocating Cylinder (Apparatus 3) (1L, 300ml,100ml & 50ml)

Apparatus 7 Reciprocating Holder (300ml,100ml & 50ml)

Dissolution Product Portfolio400-DS – Small Volume USP Apparatus 7

• Reciprocal movement (2 cm)

• 5 or 10 mL “closed” sample cells

• External jacket design to precisely control the internal temperature

• Visibility to the sample and holderwithin the cell

• Integrated sampling – includes built-in syringe pump and individual rows for sample collection

• Up to 13 samples per test

Dissolution Product Portfolio400 DS – Small Volume USP Apparatus 7

Ideal for Medical Devices or

“Combination Products”:

• Drug-coated stents / balloons

• Pacemaker leads

• Catheters

• Transdermals

• Contact lenses

• Implants

• Other novel dosage forms

Dissolution Product Portfolio850-DS Dissolution Sampling Station

• Integrated syringe pump and fraction collector – saves bench space!

• Streamlined design for ease of use and reliable, repeatable operation

• One sampler per dissolution apparatus for ultimate flexibility

• Built-in options and components for all types of dissolution methods

Dissolution Product Portfolio850-DS Features and Options

Standard:• Media replacement

• Automated cleaning

Optional:• Built-in printer• Filter module (for 0.45 or 0.2

µm filtration)

• Sample Trays

Dissolution Product Portfolio850-DS System Configurations

708-DS Dissolution Apparatus

Cary 60 UV-Vis Spectrophotometer

850-DS Sampling Station (with Filter Module)

Dissolution Workstation Software

Bio-Dis Reciprocating Holder orApparatus 7 Reciprocating Cylinder

Dissolution Product Portfolio 850-DS Features and Options

Confidentiality LabelJuly 13, 201818

Agilent Dissolution PortfolioDrug Development

Software: Dissolution Workflow Software (21 CFR Part 11 Compliance) Win UVUV ChemStationComing soon: Colligo (2019)

Qualification

280-DS

708-DS Bio-Dis

Coming soon…

Cary 8454

Cary 60 Multicell & Fiber Optic

Apparatus 7

25% Manual I

400-DSSmall volume dissolution

with autosampling

75% Automated *

Manual SemiAutomated

850-DSAutomated Sampling Station

Online

+ +

November 15, 2018

Dissolution Product PortfolioDissolution Workstation Software

Dissolution Workstation Software (DWS)

Flexible – up to 4 systems at any time.(DS708+850)x 4

Compliant – designed for 21 CFR Part 11 compliance

Efficient – paperless solution reduces manualtranscription

Secure – integrated data security with easy export features

Use Agilent’s 280-DS instrument module with DWS to monitor vibration data throughout the dissolution test and to aid in potential failure investigations!

Dissolution Product Portfolio“Sample Prep” to complement analytical finish

The Agilent Advantage

Agilent is the only dissolution manufacturer that can also provide the necessary analytical solution.

Variety of scalable systems availabledepending on customer’s needs:

•Manual: 708-DS

•Semi-automated: 708-DS + 850-DS

•Online UV Dissolution: Cary 60 or 8454

Capitalize on the strength provided by local support, training, years of expertise (VanKel > Varian > Agilent) and data management solutions.

Why Agilent?

1. Give a single Solution, from instrument, consumables and method (application note) for your analysis

2. The instrument is Robust and reliable

3. Vision Teknik give full support for Technical and Application issue

4. Low cost of ownership

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InfinityLab Columns and Supplies

• InfinityLab Quick-Connect and Quick-Turn Fittings

• InfinityLab Stay Safe Caps and Solvent Bottles

• InfinityLab FlexBench

• InfinityLab Lamps with RFID Tags

• InfinityLab Valves, Flow Cells and Pump Heads with RFID Tags

• InfinityLab Quick-Connect Heat Exchangers

• InfinityLab Poroshell Columns

• InfinityLab Column identification tags

• InfinityLab Instrument kits

• InfinityLab Maintenance kits

• InfinityLab Capillaries, Pistons, Syringes and Loops

February 20, 2017


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New and existing InfinityLab Supplies used in and around the InfinityLab LC Series giving Analytical-, Instrument- and Lab- Efficiency enhancements by Ease of Use, Safety and lowest Cost of Ownership

Full InfinityLab LC Portfolio including Bio-inert and Preparative LC

InfinityLab Columns and Supplies

April 23, 2019


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Portfolio Reminder

InfinityLab Flex Bench

InfinityLab Fittings, Parts & Consumables

InfinityLab Poroshell Columns

New InfinityLab Supplies


63

New InfinityLab Anti-Drain Fitting• Provides easy control of solvent drainage

• Routine maintenance without spilling solvent

• No introduction of unwanted air into the system.

New InfinityLab Benchtop

Most easiest module exchange!

InfinityLab Benchtop and InfinityLab Anti-Drain Fittings

Questions and Discussion

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agilent hplc infinity ii product introduction - labfarmasi.com · • it can be clustered with an...

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