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Leading the way to healthy crops.

Agdia, Inc

52642 County Road 1

Elkhart, IN 46514

Phone: 574-264-2014 or toll-free 800-622-4342

Fax: 574-264-2153

Web: www.agdia.com

Email: info@agdia.comUSER GUIDE: CymMV & ORSV ImmunoStrip®

ImmunoStrip Kit (ISK) Includes• ImmunoStrips

• SEB1 sample extracon bags

• User guide

ImmunoStrips (STX 13301/0025) purchased separately do not

include buer lled mesh bags.

What’s required to perform the assay?• Scissors, knife or razor blade

• SEB1 sample extracon buer

• Sample extracon device (Agdia sample extracon bags are

recommended)

• Leer holder or other device to hold sample extracon

bags

Intended Use

The CymMV & ORSV ImmunoStrip® test is a rapid means of screening orchids

for all strains of Cymbidium mosaic virus and Odontoglossum ringspot virus.

The CymMV & ORSV ImmunoStrip® must be used with SEB1 sample extracon

buer for leaf, ower, root and ower spike ssue. Pseudobulb is the only

part of the plant not suitable for sampling. Use both symptomac, as well as

non-symptomac ssue. Do not use any other sample extract buer.

Storage of Kit

ImmunoStrips should be stored refrigerated (4 °C) between uses and ghtly

sealed in the desiccated container at all mes. Kit contents should be warmed

to room temperature prior to use.

KIT INFORMATION

PERFORMING THE ASSAY (*Special Aenton Required)

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Collect a sample secon that is approximately

*3/4 inch square, about the size of a U.S. Quarter

Dollar. Since orchid leaves vary in thickness, it is

recommended to use a scale to weigh the ssue.

Each mesh bag contains 3 mL SEB1 buer, which

would require 0.15 g leaf, oral or root ssue; or

0.03 g of ower spike ssue.

Cut open the sample extracon bag along the

top of the label. Be careful not to spill the buer.

*SEB1 Buer is required to perform this assay.

Insert the sample between the mesh linings near

the boom of the sample extracon bag.

Extract the sample by rubbing it gently between the

mesh linings with a blunt object such as a pen or

permanent marker. Use only one sample per bag, be

sure to label each bag.

Depending on the sample type, the extract may

be too thick to wick up the strip properly. Further

diluon may be required. Contact Agdia for sampling

assistance.

Remove the ImmunoStrip from the container. When

handling the strips, always grasp the top marked

with the test name. Do not remove the protecve

covering. Insert the ImmunoStrip into the channe

poron (no mesh) of the buer lled bag. Use the

white line located below the sample arrows as a

guide for strip inseron into the liquid.

*Be sure to insert the “sample” end of the strip no

more than ¼” into the sample extracon soluon.

Allow the ImmunoStrip test to remain in the sample

extract for 30 minutes. Posive results may be visible

in as lile as 5 minutes. Lower ter samples may take

up to 30 minutes.

Prepare Sample

Perform AssayNote: If you are using a knife or razor blade to cut samples, disinfectthe cung area and cung tools with a 1:2 bleach soluon

between each sample.

(Product Numbers: ISK 13301, STX 13301)

m250.4 1

TISSUE TYPE SAMPLE DILUTION

(ssue in gram:buer volume in mL)

Leaf 1:20

Flower (Bud) 1:20

Root 1:20

Flower Spike 1:100

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RESULTS

TROUBLESHOOTING

Control line did not develop. This situaon is generally caused by over-submergence of the test strip in the sample extract.

Results in this situaon should be considered invalid and the test should be repeated.

Test runs very slow or not at all. This can be caused by using too much ssue for extracon. Repeat the test using less ssue or by

further dilung your previous sample extract 1:10 with SEB1.

If the above is not the case, make sure the test components were warmed to room temperature

before use and are within their expiraon date.

Test has a green or pigmented test line. This can be caused by using too much ssue for extracon. Repeat the test using less ssue or by

further dilung your previous sample extract 1:10 with SEB1.

**In rare cases, the ssue type may cause a pigmented line. Green lines should not be considered

a posive result. Red, orange, or purple fruits may cause what appears to be a posive test line. It

is recommended that you contact Agdia before tesng these types of samples.

Test and / or control line is weak. Make sure the test is within its expiraon date.

If kit contents were le open too long, the strips could have absorbed moisture, which can aect

test results. Be sure to always keep the ImmunoStrip vial ghtly sealed between uses.

The test line may be weak due to low pathogen ter in the sample.

m250.4 2

ImmunoStrips and sample extracon buer are non-hazardous.

SAFETY

Remove test strip from extract and interpret

results. Use the images provided as a guide to

determine results. If necessary, compare the

ImmunoStrip with the images to determine

the posions of the test and control lines. The

control line assures that the test is working

properly. If the control line does not appear, the

test is invalid.