agarose bead protein a (binds fc of igg) anti-p35 ab p35 cdk5 140,000 125,000 43,000 affinity...
TRANSCRIPT
Agarose bead
Protein A(Binds Fc of IgG)
Anti-P35 Ab
P35
Cdk5
140,000
125,000
43,000
Affinity Chromatography- Pull-down assay
AVP anti-viral product
DAI Double-stranded RNA-activated inhibitor of translation.
2’-5’-oligo-adenylate synthase dsRNA-dependent protein kinase
Viral sensing. In the cytoplasm, viral dsRNA or ssRNA containing 5'-triphosphate (ppp) is specifically recognized by RIG-I, initiating interferon production. In some cells, free viral nucleic acids, including those released from infected and killed cells, can be internalized by endocytosis and detected by Toll-like receptors in endosomes, also leading to interferon production. These mechanisms may cooperate to detect invading viruses and thence to initiate immune responses.
Science 2006, 314 p935-936.
Activated macrophages
Mycobacterial products
Latex-OVA orOvalbumin peptides
Endocytosis
Antigen processing and presentation
MHC-I restrictedCD8OVA1.3
MHC-II restrictedDOBW
MHC-IAntigen
TCRMHC-II
IL-2
IL-2
Antigen processing and presentation Assay.(Tobian A et al., 2003 J.Immunol 171 p1413.)
phycoerythrin allophycocyanin
(fluorescein isothiocyanate) (R-PE:cyanine-5)
Proteins of E. coli.
Protein with the tag of 6 neighboring histidine residues
Metal-affinity chromatography matrix:nickel-nitriloacetic acid
