M i c r o s c o p y f r o m C a r l Z e i s s

The inverted microscope Primo Vert is focused on the essentials:

Excellent optical quality, ease of use, choice of high quality materials

and an appealing design are all available at an affordable price.

Primo VertAffordable. Easy-to-Use. Ergonomic.

The new fast and convenient solution

for assessing and checking living cells.

Primo Vert addresses both routine and cutting-edge research laboratories which require compact and reliable microscopes for checking cells quickly and efficiently. The microscope can be also used by students in advanced course room settings.

Key customer benefits at a glance:• Excellent price-performance ratio • Easy-to-Use concept: auto on/off function,

universal phase contrast• Long-term mechanical and optical stability • Attractive industrial design

In addition to the binocular tube and trinocular tube (photo) versions, Primo Vert offers an innovation that generates a new class of its own: The Primo Vert Monitor.

Ultimate convenient: Primo Vert Monitor• Monitor allows multi-observation in training situations • Adjustable monitor tilt (45°- 80°)

for ergonomic and comfortable work• Head is no more in a fixed position to the eyepieces;

glasses are no longer a problem• Integrated digital camera, SD memory card slot, USB port• Remote control for the acquisition of images,

remotely or through glass plates of flow boxes

U2OS cells: Magnification 4x Ph0

One example of the numerous applications is molecular oncology

Cancer cells are cultured in incubators for the purposes of research. Their stage of develop-ment can easily be assessed with Primo Vert – a routine step that requires a consistent work-flow and fast results. The microscopic image must provide absolutely reliable information on whether the cells are adhering to the bottom of the vessel, which means they are intact and can be used in further experiments. On the other hand, if they are floating in the nutrient medium, they are stressed or already dead and thus unfit for further use.

U2OS cells: Magnification 40x Ph2

Hela-cella: Magnification 20x Ph2

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Ease of use: Walk away mode and light push-button on the stage – makes a quick check of living cells more than easy.

Increased working distance through easy removal of the condenser, e. g. for use of roller-bottles.

Focused on Essentials – Ease of Use and Reliable Results

Primo Vert presents a number of attractive details to

guarantee quick, easy and reliable handling. This makes Primo Vert

the perfect tissue culture phase contrast microscope.

The walk away mode turns off the light automatically (in addi-tion to manual on/off-function) after 15 minutes – to save energy and prolong lifetime of the bulb.

Modular illumination with halogen light or with longer lasting LEDs. The advantages of LEDs are the stable colour temperature, only one tenth in power consumption, significantly longer lifetime – therefore a lower cost solution.

Special Skin-friendly material coated on all operating elements.

Universal phase slider for all objectives: For ease of use there is one phase ring (Ph 1) available for 10x, 20x and 40x – this means no need to change phase positions when switching magnifications.

Increased working distance through easy removal of the con-denser, e. g. for roller-bottles.

Objective-indicators for fast identification of magnification.

Carrying handle on the back of the scope.

High-quality optics through high-quality glass. Objectives with infinity optics for magnification up to 40x long working distance provide high-contrast and brilliant images.

Swivelling Siedentopf-tubes. The viewing height can be ad-justed to an upper or a lower viewing height according to the preferences of the user. The inter-pupillare distance can be ad-justed down to a minimum of 48mm.

All Primo Vert versions fit well into hoods and flow boxes.

The image acquisition of Primo Vert Monitor can optionally

be operated by remote control.

Primo Vert enables discussion of the microscope image by several users.

The microscope image of the embedded camera can easily be stored on the SD card.

The Primo Vert Monitor – A New Viewing Perspective

With the new Primo Vert Monitor*, Carl Zeiss offers an ideal microscope for

multi-observation purposes. Several users can look at and discuss the microscope

image at the same time without having to adjust any eyepieces individually.

The new “pass by mode” in microscopy. Just walk and check your cell lines in the pass by mode:

• Easy inspection of living cells is possible, i.e. without hav-ing to look through eyepieces.

• Ergonomic quick check of living cells. The monitor can be tilted from 45° up to 80°. So users can easily adjust the LCD monitor to their individual posture.

• Convenient image storage by the push-to-save camera to SD memory card without PC connection. Additionally a computer can be connected to the USB port on the stand.

• No requirement to remove eyeglasses in order to check the microscopic images.

The main applications for Primo Vert are:• Sterility checks • Cell checks before protein, DNA or RNA preparation• Cell checks in the framework of screenings after donation

of substances (e. g. pharmacology) • Differentiation of cell types • Characterisation of cell lines (oncology) • Growing of cells to produce artificial tissues or organs • Documentation of the above test results

*= For research only - not for medical diagnostic.

Primo Vert – Optical and Mechanical Data

Objective change Manual via quadruple objective nosepiece

Objectives Infinity-corrected objective range with W 0.8 mounting threadPlan-Achromat: 4 x / 0.1, 4 x / 0.1 Ph0, 10 x / 0.25 Ph1LD Plan-Achromat: 20 x / 0.3 Ph1, 40 x / 0.5 Ph1, 20 x / 0.3 Ph2, 40 x / 0.5 Ph 2

Phase-Slider Universal phase slider for the objectives Ph1: Convenient and economicalPhase slider for Ph2: Higher resolution

Eyepieces WF-PL 10x/20 Br. foc.

Specimen stageDimensions (width x depth)Specimen guide Verniers with numerical and alphabetic scaleCoaxial drive

Fixed 200 x 239 mmRight sideX direction: Numerical scale, readable from right to left Y direction: Alphabetic scale, readable in the mirror right side

LD condenser 0.3 For magnifications 4 x to 40 x, WD = 72 mm

LD condenser 0.4 For magnifications 4 x to 40 x, WD = 55 mm

Binocular tube 45°/20Interpupillary distanceViewing height

Viewing angle 45°, FOV 20Adjustable from 48 to 75 mm360 to 397 mm

Trinocular (photo)tube 45°/20Photo/video portFixed beam splitting

Viewing angle 45°, FOV 20 Tube factor 1x, 60 mm50 % vis / 50 % doc

Primo Vert Monitor Camera: 5 Megapixel CMOSMonitor size: 8.4”Display: 800 x 600 pixelStorage Medium: Secure Digital (SD) card Outputs/ports: USB 2.0Camera driver for AxioVision LE with special Microscopy software: Configuration tool

Light source HAL: 6 V, 30 WLED: White light, 3 W

• All optics in Primo Vert are anti-fungus treated• Norms and standards met: CE, UL, CSA, IvD, DIN EN 61010-1 (IEC 61010-1), ISO 9001• Available optional accessories: Object guide and several mounting frames for Petri dishes etc., stage in-

serts (metal or glass), stage enlargements, several camera adapters, eyepiece micrometer and eyepiece pointer, neutral density and green interference filters, cameras, AxioVision LE microscope software

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.10Carl Zeiss MicroImaging GmbH

07740 Jena, Germany

BioSciences | Göttingen LocationPhone : +49 551 5060 660Telefax : +49 551 5060 464E-Mail : micro@zeiss.de

www.zeiss.de/micro

M i c r o s c o p y f r o m C a r l Z e i s s

Stemi 2000Stereomicroscopes

Best Optics

High Resolution

Super Zoom

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Stemi 2000 --Superior throughZeiss Perfection

Stemi 2000 stereomicroscopes meet your expecta-

tions in two respects:

They offer the cost advantage of the Greenough

design, while boasting surprising performance

features.

The ultimate in 3D imaging

Thanks to their excellent optics, Stemi 2000 stereo-

microscopes provide brilliant, sharp, distortion-free

and highly resolved images – even at the important

low magnifications.

Spectacular zoom factor of 7.7

If you are keen on minute structures, you will enjoy

the 7.7:1 zoom range. Zoom in on details, either

continuously or by click stops.

The commanding overview

The wide field of view already offered by the basic

configuration lets you overview an object area

greater than 35 mm in diameter.

The 0.3x supplementary lens expands this to 118 mm.

Burdock burrQuartz crystals

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4

Stemi 2000 with darkfieldepi-illumination and high-output KL 2500 LCDcold light source.Added option:Remote control of KL.

An advantage of the0.3x ...0.5x supplementaryzoom lens:It varies the viewing heightwithin ± 70 mm. Speciallysuited to go with cantileverand hinged-arm stands.

Stemi 2000C on the DA stand for inspecting large specimens.

Professional documentationwith the AxioCam MRc5 –pin-sharp and absolutely true to color.

A Stemi 2000 tripletStemi 2000 comes in three body versions to choose

from, depending on your requirements:

• Stemi 2000 for stereoscopic observation only

• Stemi 2000C with photo- or videocamera port

and 100/100% beam switch

• Stemi 2000CS with photo- or videocamera port

and fixed 50/50% beamsplitter

Modular and flexibleThe modular construction of the Stemi 2000

stereomicroscopes with their sensibly designed

interfaces permits a broad spectrum of uses.

Select from stands, mounting brackets and stages

to configure exactly the stereomicroscope you

require.

Light and light are not the same

Allowing epi-, transmitted and mixed illumination

techniques in bright- or darkfield, the Stemi 2000

system includes various fiber-optic illuminators

and cold-light sources of different wattages to suit

different applications.

Inspecting and archiving

The Stemi 2000C and Stemi 2000CS models can

be connected with the AxioCam MRc5 digital

microscope camera or commercial digital photo-

or videocameras. The Stemi 2000CS with its built-

in beamsplitter allows you to do observation and

documentation simultaneously.

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There is hardly a field ofapplication in which the superiorbrilliance of Stemi 2000 imageswould not prove useful.

Stemi 2000 Stereomicroscope with KL 200 Cold-Light Source

Stemi 2000Stereomicroscopes

With built-in Zeiss excellence.

Carl Zeiss Stemi 2000 Stereomicroscopes are

indisputably among the best in their class.

Their excellence in contrast, depth of field and

resolving power makes them the number one

choice for inspecting three-dimensional objects

in nature and technology, as well as in specimen

preparation for research and teaching.

Movement of a watch

Textile fibersTickPrinted circuit boardGrass seed

Specification

Subject to change.

Carl Zeiss Light Microscopy

P.O.B. 40 4137030 GöttingenGERMANYPhone: ++49 5 51 50 60 660Telefax: ++49 5 51 50 60 464E-Mail: micro@zeiss.de

www.zeiss.de/micro46-0004 e/08.03

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Stemi 2000 on stand SWeight: 4.2 kg

130

346

180 340

Supplementary lens Eyepiece

Factor Free WPL 10x/23 Br. foc WPL 16x/16 Br. foc W 25x/10 focworkingdistance (mm) Magnification Object field Magnification Object field Magnification Object field

(mm) (mm) (mm)

0.3x 285 1.95x … 15.0x 118.0 …15.3 3.1x … 24.0x 82.1 …10.7 4.9x … 37.5x 51.3 … 6.7

0.3x …0.5x 234 … 91 1.95x … 25.0x 118.0 … 9.2 3.1x … 40.0x 82.1 … 6.4 4.9x … 68.8x 51.3 … 4.0

0.4x 210 2.6 x … 20.0x 88.5 …11.5 4.2x … 32.0x 61.5 … 8.0 6.5x … 50.0x 38.5 … 5.0

0.63x 130 4.1 x … 31.5x 56.2 … 7.3 6.6x … 50.4x 39.1 … 5.1 10.2x … 78.8x 24.4 … 3.2

none 92 6.5 x … 50.0x 35.4 … 4.6 10.4x … 80.0x 24.6 … 3.2 16.3x …125.0x 15.4 … 2.0

1.25x 60 8.1 x … 62.5x 28.3 … 3.7 13.0x …100.0x 19.7 … 2.6 20.3x …156.3x 12.3 … 1.6

1.6x 48 10.4x … 80.0x 22.1 … 2.9 16.6x …128.0x 15.4 … 2.0 26.0x …200.0x 9.6 … 1.3

2.0x 31 13.0x …100.0x 17.7 … 2.3 20.8x …160.0x 12.3 … 1.6 32.5x …250.0x 7.7 … 1.0

The Essentials in Focus

M i c r o s c o p y f r o m C a r l Z e i s s

Stemi DV4Stereomicroscopes

Brilliant images and easy to use

microscope functions make

microscopy lessons a genuine

pleasure for students and

teachers alike.

High-quality optics and a variable

LED illumination in a compact unit

are important criteria when it comes

to tasks in industrial quality control.

Thanks to true-color images,

combined with the possibility

of switching between and mixing

reflected and transmitted light,

rapid and precise diagnoses can be

achieved in biological laboratories.

A fiberoptic reflected light spot

illuminator integrated into the body

of the Stemi DV4 SPOT ensures that

specimens are always well illumina-

ted – whatever the position and

direction of observation.

For Education and Routinein Science and Industry

Stemi DV4 in the classroom

Stemi DV4 with tilting mechanism being used in quality control

On a circuit board, residues of fluxing agents can be clearly identified between the contacts.

Stemi DV4 in a developmental biology laboratory

Applying protective lacquer to a plaster model in a dental laboratory

Chicken embryos intransmitted-light darkfield

Stemi DV4 SPOT on stand U in a dental laboratory

The fruit of the cocklebur “uses” hooksto attach itself to animals‘ furs, whichthen disperse it‘s seeds.

The Essentials in Focus

The innovative nature of this stereomicroscope can be seen

not only in the color and unique, compact design of

the instrument, but also in its attractive price – a result

of state-of-the-art production and assembly technologies.

Sturdy, hard-wearing technology, very easy operation of all

microscope funktions and no compromises whatsoever

in the definition and brilliance of the microscopic images – that

is the best way to describe the Stemi DV4 stereomicroscopes

from Carl Zeiss.

The pancratic system, as the heart of the stereo-microscope, has a decisive influence on the optical performance of the overall system.The Stemi DV4 is the first instrument in its class with a mechanically corrected zoom system, a feature that was previously only incorporated into stereomicroscopesin higher price categories. The result: needle-sharpimages across the entire zoom range.

Stemi DV4 stereomicroscopewith sample carousel 16A range of well-chosen accessories make the Stemi DV4an even more attractive option for a whole rangeof applications. Thanks to the sample carousel 16,up to sixteen different specimens can be positioned underthe microscope safely, quickly and extremely easily.

Stemi DV4 stereomicroscopeThe patented zoom system of the Stemi DV4 (Double Lens Vario, zoom factor 4) delivers images that are needle-sharp and, thanks to rigorous stray-light minimization, brilliant and rich in contrast. Across the entire magnification range, from 8x to 32x and across the whole field of view.

Stemi DV4 SPOT stereomicroscopeThe fiberoptic cold-light illuminator additionally integrated into the microscope body of the Stemi DV4 ensures that the object field is fully illuminated at all times. The SPOT reflected light irradiating almost vertically on the specimen, provides a shadow-free, high contrast illumination.

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Stand C LED:Combine Illumination Modesfor Outstanding Results

Stand C LED features an integrated LED illuminator for applications in reflected and transmitted light.This powerful daylight-quality illuminator shows specimens with high contrast in their natural colors. In transmitted light, six LEDs ensure bright, homogeneous illumination at any magnification, from overview to detail.

As good results in microscopy depend on

efficient illumination, we have equipped the

stand with a modern LED illuminator.

You have a choice between the following

standard features:

- reflected-light brightfield

- transmitted-light brightfield

- mixed light

In addition, there is an option for:

- transmitted-light darkfield

- polarization contrast in reflected and transmitted light

This means that you have the common illumination

modes in stereomicroscopy at your fingertips.

You can decide which ones to use and how to combine

them practically during observation – it can all be

done rapidly at the touch of a button.

At the touch of a button:rapid switching between illumination modes.

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At the touch of a button:rapid switching between illumination modes.

The ready-for-use design of the Stemi DV4 stereomicroscope with full range of illumination options as standard

Reflected-light brightfieldA standard illumination mode on a stereomicroscope, used to illuminate the surface of opaque specimens.

If a certain amount of transmitted light is added, the disruptive shadows beneath the specimen fade.

Transmitted-light brightfieldAlso a standard mode. For observing transparent specimens and contours.

If a certain amount of reflected light is added, structures on the insect’s body also become visible.

Transmitted-light dark field (optional)Extremely fine structures are illumi- nated in their natural colors against a dark background.

If a certain amount of reflected light is added, structures on the insect’s body also become visible.

Using the example of a lacewing (Chrysopa carnea, dead specimen), the different illumi-nation options that stand C LED offers can be clearly illustrated.

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The Basis of YourModular System

Measurement at a glance

Using the eyepiece micrometer you can

read measurement values directly from the

scale, either at the 8x overview or 32x

detail magnification.

Digital documentation

For the documentation of microscopic

images you can attach the camera to one

of the two eyepiece tubes in place of the

eyepiece.

Exact positioning

Quick, simple and with no risk.

Sample carousel 16, which can be retro-

fitted to stand C LED, allows you to

position up to 16 different specimens with

precision under the stereomicroscope.

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A click-stop mechanism ensures precise positioning under the microscope, whilst a low-reflection glass plate protects the specimens.

The 8x/32x/18 eyepiece micrometer is retrofitted inthe 10x standard eyepiece.

Digital compact cameras, SLR- or video cameras can be connected to the microscope quickly and easily using the appropriate eyepiece adapters.

Specifications

Front lens system

Factor FWD* (mm)

Eyepiece 10x/20 Br. foc.

Magnification Object field (mm)

0,3x

0,4x

0,3x ... 0,5x

0,63x

w/o

1,25x

1,6x

2x

287

211

233 ... 90

130

92

60

48

31

0,3x

0,4x

0,3x ... 0,5x

0,63x

w/o

1,25x

1,6x

2x

287

211

233 ... 90

130

92

60

48

31

2,4x ... 9,6x

3,2x ... 12,8x

2,4x ... 16,0x

5,0x ... 20,2x

8,0x ... 32,0x

10,0x ... 40,0x

12,8x ... 51,2x

16,0x ... 64,0x

83,3 ... 20,8

62,5 ... 15,6

39,7 ... 12,5

40,0 ... 9,9

25,0 ... 6,3

20,0 ... 5,0

15,6 ... 3,9

12,5 ... 3,1

3,6x ... 14,4x

7,6x ... 30,2x

3,6x ... 24,0x

7,5x ... 30,3x

12,0x ... 48,0x

15,0x ... 60,0x

19,2x ... 76,8x

24,0x ... 96,0x

55,4 ... 13,9

41,6 ... 10,4

55,4 ... 8,3

26,4 ... 6,6

16,6 ... 4,2

13,3 ... 3,3

10,4 ... 2,6

8,3 ... 2,1

Front lens system

Factor FWD* (mm)

Eyepiece 15x/13 Br. foc.

Magnification Object field** (mm)

Mass 5 kg

*FWD = Free Working Distance* * values based on real field number 13,3

230 300

92110

350

230 300

92110

350

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“A fundamental key to success was therefore the fact thatCarl Zeiß, right from the outset, made every effort to introduceextremely precise technology in his small workshop to ensurethat the uncertain dexterity of the human hand was subjectedto rigorous methods of testing at every point in the process.”

Carl Zeiss MicroImaging GmbH07740 Jena, Germany BioSciences, Industrial | Göttingen LocationPhone : +49 551 5060 660Telefax : +49 551 5060 464E-Mail : micro@zeiss.de www.zeiss.de/stereo

Ernst Abbe (from his commemorative speech at the Optical Workshop’s 50th anniversary celebration, given on December 12, 1896 in Jena)