Advancing Hazelnut Micro-propagation

Photo by Mark Esler

Photo by Lois Braun

About Me

Photo by Lois Braun

About Me

“Everyone blames the farmers. ‘Why don’t they change?’ But you can’t

expect a farmer to turn away from corn and soybeans if there is no

profitable alternative.” - Don Wyse

Photo downloaded from http://agronomy.cfans.umn.edu/department-directory/donald-l-wyse on May 3nd 2016

Forever Green Initiative (FGI)

Don L. Wyse, Professor and Co-Director of The Center for Integrated Natural Resources & Agricultural

Management (CINRAM)

Slide from Forever Green slideshow given to the MN legislature by Dr. Don Wyse on Feb. 4th 2015

Slide from Forever Green slideshow given to the MN legislature by Dr. Don Wyse on Feb. 4th 2015

Slide from Forever Green slideshow given to the MN legislature by Dr. Don Wyse on Feb. 4th 2015

Slide from Forever Green slideshow given to the MN legislature by Dr. Don Wyse on Feb. 4th 2015

Sub-clone four elite genotypes. 1,000 to 2,000 plants per elite genotype.

Eighteen elite genotypes in the UMN greenhouse!

Photo downloaded from http://agronomy.cfans.umn.edu/department-directory/donald-l-wyse on May 3nd 2016

Advancing Hazelnut Micro-propagation Forever Green Initiative Grant

Photo by Mark Esler

Micro-propagation Workflow

Photo by Mark Esler

Micro-propagation Workflow

Photo by Mark Esler

Micro-propagation Workflow

Photo by Mark Esler

Micro-propagation Workflow

Photo by Mark Esler

Micro-propagation Workflow

Photo by Mark Esler

Micro-propagation Workflow

Photo by Mark Esler

Micro-propagation Workflow

Caio Alves, Student Worker

Photo by Mark Esler

Micro-propagation Workflow

● Metabolomics and metabolic flux analysis

● Cell signaling and growth regulation

Horticultural Approach by the Cohen Lab

Photo downloaded from http://horticulture.umn.edu/directory/faculty/jerry-dcohen on May 3nd 2016

Jerry D. Cohen, Department of Horticultural Science

Cohen Lab

Photo downloaded from http://www.plantmetabolomics.co.nz/WhatisMetabolomics.aspx on May 4th 2016

Cohen Lab: Rooting

Photo downloaded from http://horticulture.umn.edu/directory/graduate-students/mollykreiser on May 3nd 2016

Molly Kreiser, Graduate Student

● Auxin metabolism● Induction of adventitious roots● Conversion of indole-3-butyric acid

(IBA) to free indole-3-acetic acid (IAA)

● Acclimation of hazelnuts from in vitro to ex vitro conditions

● Study of plants under stress

Cohen Lab: Plant Acclimation

Photo downloaded from http://horticulture.umn.edu/directory/staff/renata-ppincelli-souza on May 3nd 2016

Renata P. Pincelli-Souza, Post Doctoral Associate

Cohen Lab: Plant Acclimation

Left photo by Calvin Peters, Department of Horticultural Science Top right photo downloaded from https://www.arduino.cc/en/Main/ArduinoBoardDue on May 3rd 2016

Bottom right photo from K-30 datasheet manufactured by CO2meter.com

Photo by Changbin Chen, Department of Horticultural Science

Meristem Callus Culture

Left Photo by Changbin Chen, Department of Horticultural Science Right Photo by Mark Esler

Meristem Callus Culture

1)The Forever Green Initiative (FGI) seeks to make hazelnuts an economically viable crop in the midwest.

2)The FGI funds the Cohen Lab to grow 4,000-8,000, elite genotype, hybrid hazelnut plants for future field studies.

3)The Cohen Lab uses horticultural techniques to advance hazelnut micro-propagation.

Summary

Funding comes from the Minnesota Department of Agriculture

through the Forever Green Initiative

Don Wyse

Jerry CohenMolly Kreiser

Renata P. Pincelli-SouzaCaio Alves

Lois Braun

Acknowledgements

Drawing by Pierre Joseph Redoute 1833

Q&A