Advanced  Course  on  New  Methods  of  Data  Acquisition  and  Analysis  in  Biomolecular  NMR  

   

September  20-­‐23,  2010,  Swedish  NMR  Centre,  University  of  Gothenburg  http://www.nmr.gu.se/courses.html  

Registration:  contact  Vladislav.Orekhov  (at)  nmr.gu.se  

 An   intensive   hands-­‐on  workshop  will   demonstrate   how   to   combine   novel   experimental  sampling  approaches  with  spectral  analysis  and  automation  software,  and  how  to  use  the  methods  on  the  high-­‐performance  biomolecular  NMR  EU  e-­‐Infrastructure.  The  workshops  aim  is  to  cover  both  theoretical  and  practical  aspects  of  modern  tools  and  provide  hands-­‐on   training   on  NMR   instruments   and   data   processing   facilities.  Morning   lectures  will   be  complemented  in  the  afternoons  by  experiment  and  processing  sessions  in  small  groups.  

 

Speakers    

Dr.  Wolfgang  Bermel  (Bruker  BioSpin,  Germany)  Prof.  Martin  Billeter  (University  of  Gothenburg,  Sweden)  Dr.  Rasmus  H.  Fogh  (University  of  Cambridge,  UK)  Prof.  Sebastian  Hiller  (University  of  Basel,  Switzerland)  Dr.  Victor  Jaravine  (e-­‐NMR,  BMRZ  Goethe-­‐University,  Germany)  Dr.  Krzysztof  Kazimierczuk  (University  of  Gothenburg,  Sweden)    Dr.  Eriks  Kupce.  (Varian  Inc.)    Prof.  Vladislav  Orekhov  (University  of  Gothenburg,  Sweden)    Prof.  Markus  Zweckstetter  (MPI  Biophysical  Chemistry,  Göttingen,  Germany)  

Organization  Committee  

Prof.  Vladislav  Orekhov,  Swedish  NMR  Centre,  University  of  Gothenburg  Prof.  Göran  Karlsson,  Swedish  NMR  Centre,  University  of  Gothenburg  Prof.  Martin  Billeter,  Department  of  Chemistry,  University  of  Gothenburg    Dr.  Victor  Jaravine,  e-­‐NMR,  BMRZ/Goethe-­‐University,  Germany  

Notes    

• No  registration  fee    • Registration  details  will  be  posted  at  the  workshop  web  page  • The  hands-­‐on  training  limits  the  number  of  participants  to  ~20    • Requirement:  familiarity  with  protein  NMR  (assignment,  structure  

determination)  • eNMR  VO  registration  is  required  http://www.enmr.eu/eNMR-­‐

registration  • Participants  will  present  their  current  NMR  project  

 

SWEDISH NMR CENTRE

• Programme Time Mo Sep 20 12.30 – 13.00 Registration Entry hall 13.00 – 13.30 Martin Billeter, Göran Karlsson, Vladislav Orekhov Welcome and Information  13.30 – 15.00 Students presentationsi 15.00 – 18.15 Krzysztof Kazimierczuk How to (and how not to) apply Fourier Transform to non-uniformly sampled NMR signals Hands-on: processing with non-uniform Fourier transform 18.30 – 19.00 Get-together Entry hall Tu Sep 21 8.30 – 12.30 Eriks Kupce Fast Techniques in VnmrJ 10.15 – 10.45 Coffee break Library 12.30 – 14.00 Lunch 14.00 –18.00 Sebastian Hiller Automated Projection Spectroscopy (APSY): Applications to Proteins We Sep 22 8.30 – 12.00 Markus Zweckstetter Backbone resonance assignment using MARS/Analysis of dipolar couplings using PALES 10.15 – 10.45 Coffee break Library 12.00 – 13.00 Lunch

13.00 – 16.15 Martin Billeter Resonance assignments and 3D structure using spectral projections and decompositions Hands-on: PRODECOMP

16.15– 18.00 Rasmus Fogh Running programs and viewing results with CcpNmr Analysis Th Sep 23 8.30 – 10.15 Vladislav Orekhov Non-uniform sampling and Multi-Dimensional Decomposition 10.15 – 10.45 Coffee break Library 10.45 – 12.30 Wolfgang Bermel Fast methods and TopSpin 12.30 – 14.00 Lunch

14:00 – 15.30 Victor Jaravine e-NMR: Computational NMR Infrastructure in biomolecular life sciences and system biology Hands-on: work with web portals, CYANA and MDD portals

15.30 –18.00 Maxim Mayzel, Vladislav Orekhov Hands-on: NUS-MDD processing and targeted acquisition for real-time backbone assignment

Fr Sep 24 (optional follow-ups) Several course teachers are available for discussions. NMR experiments on samples brought by students

                                                                                                               i  As  a  part  of  the  course  syllabus,  each  participant  presents  himself  and  his/her  ongoing  or  planned  project,  which  is  related  to  the  topic  of  the  course.  In  addition  to  the  short  oral  presentation,  the  participants  are  encouraged  to  bring  posters  that  will  be  viewed  during  the  breaks.