adt-in-333a-002 procesamiento de western blot

8/2/2019 ADT-IN-333A-002 Procesamiento de Western Blot

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2/9

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0-7 DQMKGHAM[FBg usqacg ka ^-C- ap qm ampgyf hhqmfamihg{i`f `qgbi{g{itf ugsg bg ka{a`ì4m imTi{sf ka bfp Gm{i`qasufp dsam{a g gm{inamfp kab TIJ(7 y&f TIJ(3- Ap{g usqacg apqpgkg `fhf ampgyf `fmdishg{fsif kapuqap ka am`fm{sgs kfp usqacgp ka {ghigoaufpi{itgp-

Ugnimg 3 ka 9


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Bg usqacg q{ibbg {isgp ka mi{sfàbqbfpg kfmka pa am`qam{sgm ihusanmgkfp uau{bkfppbm{a{b`fp ka bfp tisqp JIT(7 y JIT(3 paugsgkfp aba`{sfdfsa{i`gham{a ugsg bqanfka{a`{gs bg usapamìg ka gm{i`qasufp `fm{sg usf{aimgp tisgbap apuaìdi`gp- Bgp {isgppfm bgtgkgp ugsg sahftas ab hg{asigb mf diogkf- Bfp gm{i`qasufp wqa diogmapuaìdi`gham{a bgp usf{aimgp kab JIT uqakam pas fcpastgkgp qpgmkf qmg pasia kasag`ìfmap `fm qm @fmoqngkf *gm{i`qasufp gm{i(bnN jqhgmg ka `gcsg hgs`gkfp `fmqmg dfpdg{gpg gb`gbimg) y qm pqp{sg{f C@UI&MC[- Ap{g pfbqì4m kapgssfbbgkfsg ka `fbfsuashi{a wqa bg g`{itikgk amihg{i`g kab `fhubaof gm{inamf(gm{i`qasuf diogkf pfcsa bghahcsgmg ka mi{sfàbqbfpg pa hgmidiap{a- AI kapgssfbbf kab `fbfs ap ka{amikfamoqgngmkf tgsigp taàp `fm nsgmkap `gm{ikgkap ka gnqg ka nsgkf sag`{itf-@gkg {isg im`bqya qm `fm{sfb im{asmf ka hqap{sg wqa himihig ab siapnf ka dgbpfpmang{itfp kacikf g assfsap fuasgìfmgbap y tasidi`g bg gkiì4m ka hqap{sgp-

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0-0 HG[ASIGBAP Y SAG@[ITFP( Uiua{gp ka tiksif ka 1 y 75 hb-

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0-0-7 Sag`{itfpG hamfp wqa pa imkiwqa 7 5 `fm{sgsif" pa kacam gbhgàmgs {fkfp bfp `fhufmam{apg qmg {ahuasg{qsg am{sa 3 y CF@ *cgof ap{gp `fmkiìfmap bfp sag`{itfp kab li{uashgmaàm g`{itfp jgp{g bg da`jg ka `gkqìkgk)-

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Ugnimg 0 ka 9


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dsam{a JIT(7 y JIT(3" pim sag`{itikgk ugsg ab JCpGn y pim gm{i`qasufp dsam{a gbTJ@- @fm{sfb ufpi{it} kacib7 tigb ka pqasf jqhgma img`{itgkf `fm qmg `fmàm{sgì4m cgog ka gm{i`qasufpdsam{a JIT(7 AZ@BQPITGHAM[A" pim sag`{itikgk ugsg ab JCpGn y pim gm{i`qasufpdsam{a gb JIT(3 y TJ@- [ghufm ka Cbf{{imn `fmàm{sgkf *35Z)7 dsgp`f [ghufm ka bgtgkf `fmàm{sgkf *35Z)7 dsgp`f @fmoqngkf7 dsgp`f- Gm{i`qasuf ka `gcsg gm{i-bnN jqhgmg `fmoqngkf `fm dfpdg{gpg gb`gbimg- Pqp{sg{f7 dsgp`f ka pfbqì4m ka 1(csfhf(;(`bfsf(0(imkfbib dfpdg{f *C@IU) y mi{sfgqb ka{a{sgfbif *MC[)- Ufbtf Cbf{{imn75 ugwqa{ap Z 75 9 `&q ka ba`ja kap sahgkg-

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U gnimg ; ka 9


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Kipuampa ab tfbqham maàpgsif kisa`{gham{a kab dsgp`f- Qpa qmg uiua{g bihuig-@iassa ciam ab dsgp`f kapuqap ka qpgsif- @fm{sfbap Im{asmfpBfp ap{q`jap kab sag {itf tiamam `fm pqp sapua {itfp `fm{sfbap" bfp `qgbap gtgbgm`gkg usqacg-

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Ugnimg 1 ka 9


6/9

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Am ab bicsf ka Sanip{sf y [sgcgof kigsif ugsg âp{asm Cbf{ amqhasa bgp hqap{sgp`fm ab mqhasf sapua`{itf g bg ka bg {isg ka mi{sfàbqbfpg @fbfwqa `fm bg uimg bgp {isgp am `gkg uff amqhasgkf ka bg cgmkaog ka sag`ì4m*`gmgbap sapua {itfp) G{igkg 3 hb ka [GHUFM KA BGTGKF KIBQIKF g `gkg ufìbbf ufs 7 ( 3 himq{fp g{ahuasg{qsg ghciam{a- Az{sgas ab [GHUFM y gmgkbs 3 hb ka CBF[[IMN KA [SGCGOF g `gkg ufìbbf- @fbf`gs 35 IOI ka `gkg hqap{sg y `fm{sfb sapua`{itgham{a- Im`qcgs 7 jfsg g{ahuasg{qsg ghciam{a am ubg{gdfshg cgp`qbgm{a- Bgtgs 0 taàp `gkg {isg `fm 3 hb ka [GHUFM BGTGKF KIBQIKF" kaogmkf wqa paahuguam am bg ubg{gdfshg cgp`qbgm{a am{sa bgtgkf- G{igkis 3 hb ka PFBQ@IFM KAB @FMOQNGKF KA [SGCGOF Gg `gkg ufìbbf- Im`qcgs 7 jfs{g g {ahuasg{qsg ghciam{a am ubg{gdfshg cgp`qbgm{a- Saua{is bgtgkf `fhf am ab ugpf 9- @fbf`gs ab pqp{sg{f am {fkgp bgp hqap{sgp y `fm{sfbap- Im`qcgs 71 himq{fp g{ahuasg{qsg ghciam{a am ubg{gdfshg cgp`qbgm{a- Bgtgs 0 taàp `fm gnqg nsgkf sag`{itf ugsg ka{amas bg sag`ì4m- GI {ashimgs abusfàpf pgwqa bgp {isgp ka bfp uffp `fm uimgp y `fb4wqabgp pfcsa ug{ifp ka uguaby kaoabgp pa`gs 5 kaogsbgp am bg cgmkaog jgp{g wqa pawqam ufs pi pfbgp-

Am {fkgp bgp {isgp ka sag`ì4m hqap{sg y `fm{sfbap tasidiwqa bg usapamìg ka bgcgmkg kab `fm{sfb ka pqasf Fcpasta bg usapamìg ka bgp cgmkgp satabgkgp am `gkg {isg ka mi{sfàbqbfpg am`fhugsgì4m `fm bgp {isgp ka `fm{sfb kab ampgyf

Sanip{sa bfp sapqb{gkfp am ab bicsf ka sanip{sf Uanqa bgp {isgp am ab bicsf ka sanip{sf y usf{aogbgp `fm uguab gq{fgkjapitf{sgmpugsam{a

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Ugnimg 4 ka 9


7/9

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@fm{sfb ufpi{itf kacibAp{a `fm{sfb usfufsìfmg qmg hakikg ka bg pampicibikgk kab li{- Kacam gugsaàscgmkgp kacibap am u3; y &f nu;7 y am nu735&nu745- Uqakam jgcas 5 mf gbnqmgpcgmkgp kacbbap gki ifmgbap- Bg cgmkg kab `fm{sfb ka pqasf kaca pas tipicba-0-;-; IM[ASUSA[G@IFM KA BFP SAPQB[GKFP Ugsg ati{gs assfsap am bgp im{asusa{gìfmap" bgp {isgp satabgkgp kacam ap{gs`fhuba{gham{a pa`gp- Bg usapamìg 5 gqpam ig ka gm{i qasufp dsam{a gb JIT(7 am bg hqap{sg pa ka{ashimg`fhugsgmkf `gkg {isg ka mi{sfàbqbfpg `fm bgp {isgp ka `fm{sfb kab ampgyf- [amng am `qam{g wqa ab az{sahf mqhasgkf ka bgp {isgp kacam pi{qgspa jgìg abdfmkf" ap kaìs" bgp cgmkgp nu735&nu745 pfm bgp hgp gbaogkgp kab az{sahfmqhasgkf- Bg usapamìg 5 gqpam ig ka gm{i qasufp dsam{a gb JIT(3 am bg hqap{sg pa ka{ashimgfcpastgmkf bg cgmkg apuaìdi`g kab JIT(3? cgmkg wqa gugsaà kapuqap ka bgcgmkg kab `fm{sfb ka pqasf-

} Tasidiwqa wqa bg cgmkg kab `fm{sfb ka pqasf ap tipicba- Pi ab `fm{sfb ap mang{itf" bfpsapqb{gkfp kacam `fmpikasgspa mf tgbikf" yg wqa abbf imki g qm assfs {a`mb`f" `fhf ufsaoahubf bg dgb{g ka gki i4m ka `fmoqngkf 5ka pqp{sg{f-} Ikam{idiwqa ab uapf hfba`qbgs ka `gkg cgmkg ka bg {isg ka gmgbipip q{ibigmkf `fhf nqigbgp {isgp ka `fm{sfb UFPI[ITF DQAS[A" ka `fm{sfb UFPI[ITF KACIB" 5ka ghcfp-} Bg im{asusa{gìfm ufp{asifs ka bg {isg ka gmgbipip pa cgpg am bg ka{a`ì4m ka ug{sfmapapuaìdi fp am bgp cgmkgp panqm bgp sa`fhamkg ifmap ka bgp gq{fsikgkap uas{imam{ap*Himip{asif ka Pgmikgk" Fswgmigìfm Hqmkigb ka bg Pgbqk" a{ -)-

Am bg {gcbg wqa gugsaà g `fm{imqgì4m dinqsgm gbnqmgp ka bgp kisa {si ap sa`fhamkgkgp ufskidasam{ap fsngmiphfp sanqbgkfsap im{asmg ifmgbap-

Ugnimg > ka 9


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NGN 5 UFB@am{sa Mg{ifmgba ka [sgmpdqpi4m Pgmnqima Kfp cgmkgp ka AMT `fm 5 pim

NGN 5 UFB@fmpfsìf ugsg bg mfshgbigì4m ka bgp pasfbfnigp Qmg cgmkg ka u3; 5 u07 y qmgka sa{sftisqp *@SPP) cgmkg ka AMT@sq Sfog Ap{fkfqmikampa Qmg cgmkg ka NGN" f{sg ka

UFB y f{sg ka AMTGpfìgì4m gbahgmg ugsg ab `fm{sfb ka bgp Qmg cgmkg ka AMT gb hamfpamdashakgkap tisi`gp *F^) qmg cgmkg ka NGN 5 ka UFB

Bg `gp g `fhasìgb GMMGS KIGNMFP[I@P sa`fhiamkg bgp pinqiam{ap kisa`{siàp ugsg bgim{asusa{gìfm ka bg usqacg JIT CBF[ 3-3-

UG[SFM IM[ASUSA[G@IFMMimnqmg cgmkg tisi`g apuaìdi`g usapam{a MANG[ITFKa{a`ì4m ka gm{i`qasufp u7> QMI@GHAM[A" pim mimnqmg f{sg cgmkg MANG[ITFKa{a`ì4m ka 3 AMT *nu7457 y nu735) Y NGN *u7>" u3;" u11) 5 TIJ(7 UFPI[ITFUFB *u07" u17" u44)Ka{a`ì4m ka 3 AMT *nu7457 Y nu735) y NGN *u7>" u3;" u11) 5 TIJ(7 UFPI[ITFUFB *u07 " u17" u44) Y cgmkg apuaìdi`g kab TIJ(3 tipicba `fm TIJ(3PAMGBGKF@qgbwqias cgmkg tisi`g apuaìdi`g usapam{a" uasf ab ug{s4m mf `qhuba KQKFPFbfp `si{asifp ugsg UFPI[ITF@qgbwqias cgmkg tisi`g apuaìdi`g usapam{a" uasf ab ug{s4m mf `qhuba KQKFPF `fm TIJ(bfp `si{asifp ugsg UFPI[ITF 3 PAMGBGKF@fm qmg cgmkg apuaìdi`g kab TIJ(3 tipicba-

Ugnimg : ka 9


9/9

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PIKG

0-;-1 AHIPI4M KA SAPQB[GKFP Bfp sapqb{gkfp pfm `fmpinmgkfp am ab bicsf ÂP[ASM CBF[ kab bgcfsg{fsif"sanip{sgmkf bgp cgmkgp fcpastgkgp y bg im{asusa{gì4m ka `gkg sapqb{gkf- ufpi{itf"mang{itf imka{ashimgkf Am ab PIP[AHG KA BGCFSG[FSIF pa imnsapg gb `4kinf `fssapufmkiam{a ka bghqap{sg" am dsam{a g ÂP[ASM pa `fbf`g ab sapqb{gkf UFPI[ITF"IMKA[ASHIMGKF 5 MANG[ITF" ugsg `fbf`gs bgp cgmkgp wqa pa fcpastgm am bgàbkg `fssapufmkiam{a g GM[I@QASUFP @FSA" GM[I@QASUFPAMKFMQ@BAGPG UFbb HASGPG Y GM[I@QASUFP AMTFB[QSG ufs ubgm{ibbg"imnsapf ka ubgm{ibbgp paba`ìfmgs bg cgmkg g `fbf`gs bfp pinmfp ( 4 , pawqm bgusapamìg 5 mf ka ap{g- Tgbikgs a ihusihis-

0-;-4 BbHI[G@IFMAP KA BG [a@MI@GBg ka{a`ì4m ka gm{i`qasufp dsam{a gb TIJ(7 mf ihubi g wqa pa jgyg ap{gcba ikf qmkignm4p{i`f ka pimksfha ka imhqmfkadiìamìg gkwqisikg *PIKG)- Qm sapqb{gkfmang{itf mf kap`gs{g bg ufpicibikgk ka imda` i4m-Ap qm ja`jf `fmfìkf wqa bgp uaspfmgp wqa jgm azuasiham{gkf qmgpasf`fmtaspi4m saìam{a uqakam usapam{gs qm ug{s4m im`fhuba{f" uasf pa usfkqàqm gqham{f ka sag`{itikgk *{gm{f am ab mqhasf `fhf am bg im{ampikgk ka bgpcgmkgp) pi pa jgà qm panqihiam{f kqsgm{a qm uasifkf ka kfp g {sap hapap- Bghgyfsig ka bgp {sgmpdasamìgp `fm sapqb{gkf UFPI[ITF usapam{g f{sgp cgmkgptisi gp apuaìdi gp-Bgp usqacgp IMKA[ASHIMGKGP mf kacam q{ibigspa `fhf cgpa ugsg abkignm4p{i`f ka imda`ì4m ufs TIJ(7 Pa sa`fhiamkg saua{is {fkgp bgp usqacgp `fmsapqb{gkf IMKA[ASHIMGKF q{ibigmkf bg hqap{sg fsinimgb y hqap{sgp pasigkgp- Ap{ghciam pgcikf wqa bfp gm{i`qasufp dsam{a g u3; y u07 kiphimqyam kqsgm{a ab`qspf kab PIKG" 7 5 wqa usftf`g qm kapubgghiam{f ka bg im{asusa{gì4m ka bgusqacg ka UFPI[ITG g IMKA[ASHIMGKF- Ufs `fmpinqiam{a" am {gbapìs`qmp{gmìgp" bg im{asusa{gì4m ka bfp sapqb{gkfp kaca cgpgspa am bgp usqacgpufp{asifsap y am bgp atgbqgìfmap `bimi`gp-Kgkg pq abatgkg apuaìdi ikgk" bg GQPAM@IG KA SAG@[ITIKGK ka bgp hqap{sgp`fm ab uau{ika apuaìdi`f ka bg amtfb{qsg kab TIJ(3 am qmg usqacg kqkfpg mfaz`bqya bg ufpicibikgk ka imda` i4m `fm f{sgp àugp kab TIJ(3-

Ugnimg 9 ka 9

adt-in-333a-002 procesamiento de western blot

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