NanoEnTek Inc.

ADAM-SCCAuto-microscopic Somatic Cell Counter

www.nanoentek.com

• Automated fluorescent microscope + Disposable micro plastic chip• Compatible to the Direct Microscopic Somatic Cell Count•Test tool kit can be provided for user’s convenience• Composed of Device, Somatic Cell count Kit

ADAM-SCC

Somatic Cell count Kit

www.nanoentek.com

DMSCC, 1000 × ADAM-SCC

SCC = 4,770,000 cell/ml

DNA labeling Cell Count: PI staining method• Somatic cell count• Cell viability test / mammalian cell count

Real-time live image view• 4 × objectives, Fluorescent images

www.nanoentek.com

200 400 600 800 1000

200

400

600

800

1000 y = 1.045x - 14.038

R2 = 0.99

C-R

eade

r (X1

,000

/ml)

Eastern Lab. Standard solution (X1,000/ml)200 300 400 500 600 700

200

300

400

500

600

700 y = 1.04x - 12.16

R2 = 0.99

C-R

eade

r (X1

,000

/ml)

Standard Sol. (X1,000/ml)

Correlation between 12 standard somatic cell solution (Eastern Lab., USA) and ADAM-SCC

Correlation between 3 kind standard somatic cell solution (NVRQS, Korea) and ADAM-SCC

Validation & Verification

Accuracy for somatic cell standard solution

By National Veterinary Research Quarantine Service (Korea)

www.nanoentek.com

Functional Comparison of Competitors

Feature ADAM-SCC DMSCCHigh-Throughput

EquipmentD Company

Process Time Fast Very slow Very Fast Fast

Personal Error No Yes No Yes

Method Similar Standard Method Standard Method Flow cytometry Similar Standard Method

Repeatability <5% at 100,000 / mL - <5% at 100,000 / mL <12% at 100,000 / mL

Sample Prep. Simple Complex Simple Simple

Measure Cell size Yes Yes No No

View Cell Morphology Yes Yes No No

Calibration No - Need No

Cell Types Somatic Cell Most Cells Somatic Cell Somatic Cell

Throughput High Low High High

Device Price Low - High Low

Peripheral Device Chip, Stain Solution Microscope, Reagent Many Cassette, Lysis Buffer

Device Size Portable - Big Small

Maintenance Coast Low - High Low