ACTIPORINE 8.G

Anti-Ageing - Mitochondrial Detox

JANIA RUBENS

Jania comes from the Latin Janus, the two-headed god of Roman

mythology, guardian of the House of the Gods. Rubens means the

colour red. The alga was given this name due to its dichotomous

branching and its colour. Jania Rubens is a calcified alga that grows

from 15 to 40 mm in height. It is fairly rare and represents a sort of

tree of life where, thanks to its calcified structure, a wide range of

microscopic species finds a protection.

To prevent removal of this rare resource from its natural habitat, our

laboratory has been the first one to develop cultures in a

photobioreactor with controlled temperature, light and culture

media. The algae obtained using this method have the shape of a

pompom, similar to that observed in their natural habitat, but

without any epiphytes. The extract obtained from it: Actiporine 8G is

guaranteed to be 100% natural and to have no traces of impurities.

In heart cells, mitochondria plays a central role in their vitality and activity. In fibroblasts, a mitochondrial

dysfunction results in a decrease in collagen synthesis and acceleration of skin ageing process. ACTIPORINE 8G

provides a unique mechanism of mitochondrial detoxification to boost collagen synthesis and to reduce the

appearance of wrinkles.

TECHNOLOGICAL INNOVATION: THE CULTIVATION OF JANIA RUBENS

A UNIQUE ACTION MECHANISM

ROLE OF MITOCHONDRIA IN COLLAGEN SYNTHESIS

Collagen synthesis is connected to the integrity, vitality and thus

energy reserves of the fibroblasts. However the only source of energy

available to the cells is ATP, which is synthesised by the mitochondria.

IMPORTANCE OF MITOCHONDRIAL DETOXIFICATION

It is now well known that mitochondrial DNA, due to its closeness to

reactive oxygen species, and mainly H2O2, is continually subject to

damages and mutations leading to mitochondrial malfunction and loss

of cellular vitality.

However H2O2 is a messenger of vital importance in a large number of

metabolic reactions. Therefore it is important to remove H2O2 from

the mitochondria without, however, completely eliminating it.

THE ROLE OF AQUAPORIN 8 IN MITOCHONDRIAL DETOXIFICATION

It has been shown that Aquaporin 8 (AQP8), membrane channel

already known for its role in the transport of urea, is also capable to

ensure the passage of H2O2 through the mitochondrial membrane.

THE ANTI-AGEING STRATEGY DEVELOPED BY ACTIPORINE 8G IS BASED

ON AN INCREASE IN THE NUMBER OF AQP8 TO PROMOTE H2O2

EVACUATION, MITOCHONDRIAL DETOXIFICATION AND THEREFORE

THE ACTIVATION OF COLLAGEN SYNTHESIS BY FIBROBLASTS.

Aquaporin

IN-VITRO TESTS

IN-VIVO TESTS

Protocol : Normal human dermal fibroblasts (37 years old women)

treated with 0.2% Actiporine 8 G for 24 hours.

ACTIPORINE 8G INCREASES AQP8 SYNTHESIS BY +58%*.*p<0.05 Student test,

ACTIPORINE 8G ACHIEVES 100% MITOCHONDRIAL DETOX

The basal rate measurement of H2O2 in the mitochondria showed a 17%

accumulation of H2O2 under conditions of cellular stress (induced

release of H2O2). Under these conditions, only Actiporine 8G is capable

of removing 100% of accumulated H2O2. Recycling H2O2 outside the

mitochondria is a strategy which is more efficient than the use of a

powerful anti-oxidant such as Vitamin E.

ACTIPORINE 8G REACTIVATES COLLAGEN SYNTHESIS+24% de collagen I

+28% de collagen IV

+51% de collagen 7

Visualization (in red) of H2O2

accumulation inside the

mitochondria of untreated

fibroblasts [A], fibroblasts

treated with H2O2 inducer

[B], and fibroblasts treated

with H2O2 inducer +

Actiporine 8G [C]

A

CB

ACTIPORINE 8G INCREASES COLLAGEN DENSITY IN DERMISProtocol : 20 volunteers. Application of a cream containing 2% Actiporine 8 G

on the thighs, twice daily for 4 and 8 weeks. Analysis of collagen synthesis in

dermis by echography

AFTER 28 DAYS: +5% COLLAGEN DENSITY AND UP TO +61%

AFTER 56 DAYS: +15%* COLLAGEN DENSITY AND UP TO + 54%

*p<0.05 Student test

ACTIPORINE 8G DECREASES CROW’S FEET WRINKLES IN 14 DAYSProtocol: 20 volunteers. Application of a cream containing 2% Actiporine 8G

or a placebo, on crow’s feet wrinkles twice daily for 2 weeks.

ROUGHNESS: -5,1%* AND UP TO -44%

WRINKLE VOLUME: -11,5% AND UP TO -85%

WRINKLE AREA: -9,4% AND UP TO -57%

*p<0.05 Student test

H2O2 RECYCLING THANKS TO AQP8 ALLOWS A COMPLETE

DETOXIFICATION OF MITOCHONDRIA AND A REACTIVATION OF

COLLAGEN SYNTHESIS FOR VISIBLE RESULTS ON CROW’S FEET

WRINKLES AFTER ONLY 2 WEEKS.

Visualization in green of

collagen density in the dermis,

before treatment [A], after 4

weeks treatment [B] and after

8 weeks treatment [C]

A

CB

ACTIPORINE 8G – ANTI-AGEING & MITOCHONDRIAL DETOXIFICATION

COSMETIC ACTIVITIES

• Activates the synthesis of mitochondrial AQP8 in fibroblasts

• Detoxifies mitochondria of fibroblasts

• Increases the synthesis of collagen I, IV and VII

• Improves collagen density in dermis

• Decreases skin roughness

• Decreases volume and surface of wrinkles within 2 weeks

INCI NAME

Glycerin (and) Water (and) Jania Rubens extract - !OK CHINA!

RECOMMENDED % OF USE: 2%

Technical department : infotech@codif-recherche-et-nature.com

Commercial department : commercial@codif-recherche-et-nature.com

70, rue du Commandant l’Herminier – CS11781 - 35417 Saint-Malo cedex - FRANCE

Tel : +33-2-23-18-31-07 Fax : +33-2-23-18-31-01

www.codif-recherche-et-nature.com