INSTITUTE OF

VIROLOGY

With the support ofOrganizersUnder the auspices

FUNDATIA ACADEMICIAN“NICOLAE CAJAL”

INSTITUTUL DE CERCETĂRI ȘTIINȚIFICE MEDICALE

„ACADEMICIAN NICOLAE CAJAL”

OCTOBER 17 - 19, 2019

XIV edition

ACADEMICIAN NICOLAE CAJAL SYMPOSIUM

ROMANIAN ACADEMY LIBRARY

˛

FINAL PROGRAM

EDITION DEDICATED TO THE MEMORY OF ACAD. NICOLAE CAJAL

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ACKNOWLEDGEMENTS TO

Thursday, 17 October 2019Aula Magna of the Romanian Academy

10.00 – 18.00 REGISTRATION Foyer the Romanian Academy Library

11.00 – 14.00 SESSION DEDICATED TO THE MEMORY OF ACAD. NICOLAE CAJALAula Magna / Romanian Academy

Victor Voicu President, Medical Sciences Section of the Romanian Academy and Vice-President of the Romanian Academy

Razvan Theodorescu Vice-President of the Romanian Academy

E.S.Mr. David Saranga Ambassador of Israel in Romania

Stefan N. ConstantinescuLudwig Institute for Cancer Research, Brussels and Vice President of the Federation of European Academies of Medicine (FEAM)

Carol Iancu „Paul Valery” University – Montpellier III, France

Costin Cernescu Honorary Director, “Stefan S. Nicolau” Institute of Virology

Irinel Popescu President, Romanian Academy of Medical Sciences

Irina Cajal Marin President, Academician Nicolae Cajal Foundation

14.00 – 15.00 Visit to Acad. Nicolae Cajal House Educational Complex Lauder Reut (Leul Ierusalimului Square , 12 Cluceru Udricani Str.,Bucharest - S3)

Departure by buses from the Romanian Academy (max. 80 places)

16.00 – 17.00 OFFICIAL OPENING CEREMONY I.E. Radulescu Hall / The Library of the Romanian Academy

Irina Cajal Marin President, Academician Nicolae Cajal Foundation

Irinel Popescu President, Romanian Academy of Medical Sciences

Stefan ConstantinescuLudwig Institute for Cancer Research, Brussels and Vice-President of the Federation of European Academies of Medicine (FEAM)

Carmen Diaconu Director, “Stefan S. Nicolau” Institute of Virology

SCIENTIFIC PROGRAM

17.00 – 18.30 SESSION I Chairs: Prof. Dr. Irinel Popescu, Prof. Stefan Constantinescu

L1. CELL REPLACEMENT THERAPY FOR DIABETICS BY LIVER TO PANCREAS TRANSDIFFERENTIATION1,6-9 1,6-8 3 3, 6 1,6 1,6

Sarah Ferber , Irit Meivar-Levy , Daniela Lixandru , Simona Dima , Ioana Raluca Florea , Veronica Madalina Ilie , 1,2,5,6 6 1,3,6Radu Albulescu , Ioan Valentin Matei , Irinel Popescu

1 2Titu Maiorescu University, Cajal Institute, Bucharest, Romania; National Institute for Chemical Pharmaceutical R&D,

3 4 5Fundeni Clinical Institute, Stefan S. Nicolau Institute of Virology, Victor Babes National Institute of Pathology, Bucharest,

6 7 8 9Romania; DiaCure project, Sheba Medical Ctr. Orgenesis Ltd, Tel Aviv University

L2. 50 YEARS OF REVERSE TRANSCRIPTASE - RETROVIRUSES AND BEYONDKarin MoellingUniversity of Zurich, Zurich,CH and MPI Mol Genetics, Berlin, DE and Honorary Director of Biotechnology, Stefan S. Nicolau Institute of Virology, Romanian Academy

L3. IN SEARCH OF NORMAL ARTHERIAL BLOOD PRESSURE Nanea Ioan Tiberiu , Cristina ElenaDinu , Ligia Moldovan1 2 1

1Internal medicine and cardiology, “Prof. Dr. Th. Burghele” Hospital Bucharest, Romania2Deputy, Secretary of the Health and Family Comission, Chamber of Deputies

19.00 WELCOME DRINK Foyer of the Romanian Academy Library

SCIENTIFIC PROGRAM

Friday, 18 October 2019I.E. Radulescu Hall / The Romanian Academy Library

09.00 – 11.00 SESSION II Chair: Simona Ruta

L1. ONCOVIR- EXCELLENCE RESEARCH PLATFORMGabriela Anton, Simona Ruta, Lorelei Brasoveanu, Carmen DiaconuStefan S Nicolau Institute of Virology

L2. WEST NILE VIRUS INFECTION IN ROMANIA, 1996-20191,2 1,3 1,2 2 1,2

Simona Ruta , Corneliu Popescu , Camelia Sultana , Andrei Cimpan , Aura Temereanca , consortiul VectExcel1 2 3Carol Davila University of Medicine and Pharmacy, Stefan S Nicolau Institute of Virology, Victor Babes Infectious Diseases Hospital

L3. RECONSTRUCTING THE EVOLUTIONARY HISTORY OF HIV-1 SUBTYPE F1: A MOLECULAR EPIDEMIOLOGICAL APPROACH

1 1 1 1 1Simona Paraschiv* , Leontina Banica , Ionelia Nicolae , Mihaela Fratila and Dan Otelea1Molecular Diagnostics Laboratory, National Institute for Infectious Diseases “Matei Bals”, Bucharest, Romania

L4. THE ROLE OF E6 AND E7 HPV16 ONCOPROTEINS IN HOST CHROMATIN REMODELLING THROUGH COMPONENTS OF NURD COMPLEXA. Botezatu, I.V. Iancu , M. Bostan , M. Mihaila, A. Albulescu, A. Fudulu, C. Diaconu, G. Anton, A. Plesa Stefan S. Nicolau Institute of Virology, Bucharest, Romania

11.00 – 11.15 COFFEE BREAK / POSTER VIEWINGFoyer of the Romanian Academy Library

11.15 – 13.15 SESSION III Chair: Carmen Diaconu

L1. MOLECULAR PSYCHIATRY TRANSLATED INTO TREATMENT RESPONSE AND DIAGNOSIS. RECENT INVOLVEMENT OF THE IMMUNE SYSTEM

1 2 2Maria Grigoroiu-Serbanescu , Ana Iulia Neagu , Carmen C. Diaconu1 2 Psychiatric Genetics Research Unit, Alexandru Obregia Clinical Psychiatric Hospital, Bucharest, Romania, Stefan S Nicolau

Institute of Virology, Bucharest, Romania

L2. PLASMA PROTEIN ANALYSIS IN PHILADELPHIA-NEGATIVE MYELOPROLIFERATIVE NEOPLASMS1 1 2 1 1 3 3

Cristina Mambet , Laura G.Necula , Simona Mihai , Lilia Matei , Ana Iulia Neagu , Gabriela Mocanu , Oana Stanca , Irina 3 3 3 4 5 6Triantafyllidis , Raluca Manolache , Andrei Colita , Aurelia Tatic , Gabriela Calotă , Mihai Alexandru Sotcan , Viola

7 8 1 1 3 8Popov , Horia Bumbea , Mihaela Chivu-Economescu , Coralia Bleotu , Nicoleta Berbec , Ana-Maria Vladareanu , Anca-

3 4 2 1 1,9Roxana Lupu , Daniel Coriu , Cristiana Tanase , Carmen C. Diaconu , Stefan N. Constantinescu1 2Cellular and Molecular Pathology Department, Stefan S. Nicolau Institute of Virology, Bucharest, Romania, Biochemistry-

3Proteomics Laboratory, "Victor Babes" National Institute of Pathology, Department of Hematology, Coltea Clinical

4Hospital, Bucharest, Romania, Center of Hematology and Bone Marrow Transplantion, Fundeni Clinical institute,

5 6Romania, Hematology Outpatient Unit, CF 2 Clinical Hospital, Bucharest, Romania, Hematology Unit, "Carol Davila" 7 8Military University Hospital, Bucharest, Romania, Hematology Unit, Colentina Clinical Hospital, Hematology Unit, 9

Emergency University Hospital, Bucharest, Romania, University catholic of Louvain, de Duve Institute, Ludwig Institute for Cancer Research, Brussels, Belgium

L3. MOLECULAR CHARACTERIZATION OF PANCREATIC DUCTAL ADENOCARCINOMA USING FINE NEEDLE ASPIRATION 1 1,2,3 1 2,3 2,3 1Ioana Manea , Razvan Iacob , Andrei Sorop , Stefania Bunduc , Bianca Stoica , Ana Maria Pantazica , Alina

1 1 1 1 1,2 1,2,4 1,2,3Ghionescu , Diana Constantinescu , Elena Moise , Maria Sirbu , Simona Dima , Irinel Popescu , Cristian Gheorghe 1 2 Centre of Excellence in Translational Medicine, Fundeni Clinical Institute, Digestive Diseases and Liver Transplantation Center,

3 4Fundeni Clinical Institute, ”Carol Davila” University of Medicine and Pharmacy, Acad. Nicolae Cajal Institute of Medical Scientific Research, Titu Maiorescu University Bucharest

SCIENTIFIC PROGRAM

L4. HEPATOCYTE NUCLEAR FACTOR 4 ALPHA - A BIOMARKER FOR PROGNOSIS IN HEPATOCELLULAR CARCINOMA AND A POTENTIAL TARGET GENE FOR DIFFERENTIATION THERAPY

1,2,3,4 4 4 2 1,2,3 1,2,5 1,2,5Razvan Iacob , Urda Ruedrich , Michael Rothe , Veronica Ilie , Speranta Iacob , Vlad Herlea , Adina Croitoru , 1,2 1,2,3 1,2,3 4 4 4*

Simona Dima , Cristian Gheorghe , Liana Gheorghe , Axel Schambach , Michael Manns , Michael Ott Irinel ,1,2,5Popescu

1 2 Digestive Diseases and Liver Transplantation Center, Fundeni Clinical Institute, Bucharest, Romania, Center of 3

Excellence in Translational Research, Fundeni Clinical Institute Bucharest, Romania, University of Medicine and Pharmacy 4 5 “Carol Davila”, Bucharest, Romania, Hannover Medical School, Hannover, Germany, University of Medicine and Pharmacy

“Titu Maiorescu”, Bucharest, Romania

13.15– 14.15 LUNCH BREAK Foyer of the Romanian Academy Library

14 .15 – 16.15 SESSION IV Chairs: Prof Sarah Ferber, Simona Dima

L1. ESTRADIOL-PRIMED HUMAN MESENCHYMAL STROMAL CELLS PRIMED WITH ESTRADIOL ENHANCE ANGIOGENESIS IN HEART TISSUE

1 1 1 1 3 2,3Maria Cristina Mihai ,Mirel Adrian Popa , Viorel Iulian Suica ,FeliciaAntohe ,Edwin K Jackson , Raghvendra K Dubey , 1

Maya Simionescu ,1Institute of Cellular Biology and Pathology “Nicolae Simionescu” of the Romanian Academy, Bucharest, Romania;2Department for Reproductive Endocrinology, University Zurich, Switzerland;3Department of Pharmacology and Chemical Biology, University of Pittsburgh, USA

L2. NEW EPITOPES ON TUMOR CELLS AS TARGET FOR CELLULAR IMMUNOTHERAPIESVirgil Paunescu, Oana Gavriliuc, Roxana Zogorean, Alexandru Tirziu, Florina BojinVictor Babes University of Medicine and Pharmacy Timisoara and OncoGen Centre Timisoara

L3. DIAGNOSTIC ROLE OF CIRCULATING COL10A1 IN GASTRIC CANCER 1,2 1 1 1 1 1Laura Georgiana Necula , Lilia Matei , Denisa Dragu , Ana Iulia Neagu , Ioana Pitica , Cristina Mambet , Coralia

1 3 3 1 1Bleotu , Simona Dima , Irinel Popescu , Carmen C. Diaconu , Mihaela Chivu-Economescu1 2Department of Cellular and Molecular Pathology, Stefan S. Nicolau Institute of Virology, Bucharest, Romania; Titu

3Maiorescu University, Faculty of Medicine, Bucharest, Romania; Fundeni Clinical Institute, Bucharest, Romania

L4. PHENOTYPIC ASSESSMENT OF LIVER-DERIVED CELL CULTURES DURING IN VITRO EXPANSION; DONOR'S AGE RELATED DIFFERENCES

1,2,3 1,2,4 1,4 1,4 1,4 4-6Razvan Iacob , Vlad Herlea , Lorand Savu , Ioana Raluca Florea , Veronica Ilie , Irit Meivar-Levy , Ioan Valentin

1,4 1,3 1,2,4 4 1,2,4 4-6Matei , Daniela Lixandru , Simona Olimpia Dima , Radu Albulescu , Irinel Popescu , Sarah Ferber1 2Center of Excellence in Translational Medicine, Fundeni Clinical Institute, Bucharest, Center for Digestive Diseases and Liver

3 4Transplantation, Fundeni Clinical Institute, Bucharest, ”Carol Davila” University of Medicine and Pharmacy Bucharest, Dia-

5Cure, Acad. Nicolae Cajal Institute of Medical Scientific Research, Titu Maiorescu University Bucharest, The Sheba 6Regenerative Medicine, Stem Cell and Tissue Engineering Center, Sheba Medical Center, Tel-Hashomer, Orgenesis Ltd.

L5. THE EFFECT OF LIVER CELL DONOR AGE, GENDER AND METABOLIC STATE ON TRANSDIFFERENTIATION EFFICIENCY TO ENDOCRINE PANCREAS

5* 1,5-7* 3 3,5 1,5Ioan Valentin Matei , Irit Meivar-Levy , Daniela Lixandru , Simona Dima , Ioana Raluca Florea , Veronica 1,5 1,2,4,5 1,5-8 1,3,5

Madalina Ilie , Radu Albulescu , Sarah Ferber , Irinel Popescu1 2Titu Maiorescu University, Cajal Institute, Bucharest, Romania; National Institute for Chemical Pharmaceutical R&D;

3 4 5 6Fundeni Clinical Institute, Victor Babes National Institute of Pathology, Bucharest, Romania; DiaCure project; Sheba 7 8

Medical Ctr. ; Orgenesis Ltd; Tel Aviv University

16.15 – 16.30 COFFEE BREAK/ POSTER VIEWING Foyer of the Romanian Academy Library

SCIENTIFIC PROGRAM

16.30 – 18.00 SESSION V Chairs: Cristiana Tanase, Virgil Paunescu

L1. HYPERLIPIDEMIA INDUCES THE PRODUCTION OF DYSFUNCTIONAL HDL IN THE SMALL INTESTINE AND LIVER OF HAMSTERS, AND INCREASES HEPATIC LIPID-RELATED miRNASTeodora Barbalata, Loredan S. Niculescu, Mihaela G. Carnuta, Mina Raileanu, Anca V. Sima, Camelia S. Stancu Lipidomics Department, Institute of Cellular Biology and Pathology “Nicolae Simionescu” Bucharest, Romania

L2. CAR-BASED IMMUNOTHERAPY WITH ANTI-CD19 AND ANTI-EGFR T CELLS AND NK CELLSPaunescu VirgilOncoGen Center

L3. EMPOWERING THE STEM CELL-BASED STRATEGIES FOR CARDIAC REGENERATION BY DUAL CELL THERAPYAlexandrina Burlacu, Maya SimionescuInstitute of Cellular Biology and Pathology “Nicolae Simionescu” Bucharest, Romania

L4. PROTEOMIC APPROACHES FOR EVALUATION OF HEPATOCYTE TRANSDIFFERENTIATION INTO PANCREATIC BETA CELLS1,2,5 1,2 3 3 1,2,6,7 1 1

Cristiana Tanase , Radu Albulescu , Daniela Lixandru , Simona Dima , Irit Meivar , Raluca Florea , Veronica M. Ilie , 1,4 1,2,3 1,2,6-8Laura Georgiana Necula , Irinel Popescu , Sarah Ferber

1 2 3 4 Titu Maiorescu University - Cajal Institute Bucharest Romania, DiaCure project, Fundeni Clinical Institute, Stefan S.

5 6Nicolau Institute of Virology, Victor Babes National Institute of Pathology Bucharest Romania, Sheba Medical Ctr., 7 8Orgenesis Ltd, Tel Aviv University

SCIENTIFIC PROGRAM

Saturday, 19 October 2019I.E. Radulescu Hall / The Romanian Academy Library

09.00 – 11.00 SESSION VI Chairs: Sanda Maria Crețoiu, Razvan Iacob

L1. IMPACT OF INTESTINAL MICROBIOME ON HEALTH AND CHRONIC GASTROINTESTINAL DISEASES1,2

Sanda Maria Crețoiu1Department of Cell and Molecular Biology and Histology, Carol Davila University of Medicine and Pharmacy, 050474

Bucharest, Romania2 thClinica Nutriscience, 18 Pictor Barbu Iscovescu, 011937 Bucharest, Romania

L2. Rigenera – Innovative Micrografting Technology Bogdan IvanescuStem Sure – British stem cells bankDoctor MYTH – media medical platform

L3. HPV INFECTION IN PREGNANT WOMEN AND ITS ROLE IN PREGNANCY OUTCOME1 1 1,2 3,4

Florentina Duica , Carmen Elena Condrat , Dragos Cretoiu , Nicolae Suciu 1Alessandrescu-Rusescu National Institute of Mother and Child Health, Fetal Medicine Excellence Research Center,

Bucharest, Romania2Department of Cellular and Molecular Biology and Histology, Carol Davila University of Medicine and Pharmacy,

Bucharest, Romania3Department of Obstetrics and Gynecology, Polizu Clinical Hospital, Alessandrescu-Rusescu National Institute of Mother

and Child Health, Bucharest, Romania4 Department of Obstetrics, Gynecology and Neonatology, Carol Davila University of Medicine and Pharmacy, Bucharest,Romania

L4. ACUTE MYOCARDICAL INFARCTION IN SEPSIS: BENE DIAGNOSTICITUR – BENE CURATUR? 1 3 1Camelia Nicolae , Ligia Moldovan, Corina Grițac , Ioan Tiberiu Nanea

1Carol Davila University of Medicine and Pharmacy, Bucharest;

2 Prof. Dr. Theodor Burghele Hospital, Bucharest;3National Institute of work expertise, Bucharest

L5. PROGNOSTIC FACTORS OF SHORT-TERM MORTALITY FOR PATIENTS WITH CONGENITAL DIAPHRAGMATIC HERNIA1,2

Andreea-Madalina Serban1Center of Excellence in Translational Medicine, Fundeni Clinical Institute, Bucharest, Romania 2Carol Davila University of Medicine and Pharmacy, Bucharest

11.00 – 11.15 COFFEE BREAK/ POSTER VIEWING Foyer of the Romanian Academy Library

11.15 – 13.15 SESSION III Chairs: Manole Cojocaru, Anca Hancu

L1. BROWNIAN AND KINETIC MOTION OF MICROBIAL FLORA IN PARODONTOPATIA Sabau RalucaUMFST -Tg .Mures

L2. HUMAN VIROME AND INFLAMMATION ASSOCIATED DYSBIOSISSimona Soare , Manole Cojocaru , Cristina Mocanu

1 1 2

1Titu Maiorescu University, Faculty of Medicine, Bucharest;2Carpatia Group, Rockville Faculty of Medicine/University of Maryland.

SCIENTIFIC PROGRAM

L3. POTENTIAL MARKERS OF THE RAPID COGNITIVE DECLINE CONSECUTIVE OF MINOR BRAIN INJURIES- THE TRANSLATIONAL PERSPECTIVE

1 2 3 4 5Dragos Marinescu , Laurentiu Mogoanta , Carol Friedman , Olivian Stovicek , Ileana Marinescu1University of Medicine and PharmacyCraiova, Doctoral school

2University of Medicine and Pharmacy Craiova, Departament of Morphopathology3„Ovidius”University Constanta, Faculty of Medicine, Doctoral school4„Titu Maiorescu”University Bucharest, Faculty of Medical Assistance Tg. Jiu, Department of Pharmacology

5University of Medicine and Pharmacy Craiova, Department ofPsychiatry

L4. HUMORAL AORTIC VASODILATATIVE FACTOR Zeana Corneliu, Caius Traian Dragomir, Monica Botea, Daria CalinUniversity of Medicine and Pharmacy “Carol Davila”, Bucharest, Romania“Victor Babes Institute, Bucharest

L5. GUT MICROBIOTA AND EATING DISORDERS 1 2Anca Hancu ; Florin Mihaltan

1Nutriscience Clinic

2Carol Davila University of medicine and pharmacy

L6. EVENTS IMMUNE-ALLERGIC EDIBLE INSECTS Sinitchi GeorgetaAllergy Medical Center Atopia

13.15 - 14.15 CLOSING CEREMONY I.E. Radulescu Hall / The Romanian Academy Library

SCIENTIFIC PROGRAM