On-Line Calculation of Plate Number and Resolution of a Chromatograohic Column

82.

Kan it Krissanangkul*

Abstract

..

GC Plate Program is a Basic Program used in C-R3A data processor.

This program would check temperature and baseline of a gas chromatograph

for readiness prior to sample injection. Plate number resolution and

percentages of- each component in the injected sample were calculated.

Percentages ~aleulated by GC plate program were much closer to the

actual values th·an those calculated by the program provided by the

man uf act urer.

* School of Energy and Materials. King Mongkut 's Institute of Technology

T.honburi •.

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1. McNair HM and Bonelli EJ. in "Basic Gas Chromatography" Varian

Instrument 1969, p. 158.

• .>

•

2. Freedman B and Pryde EH in "Vegetable Oil Fuels" Proceeding of •

• the International Conference on Plants and Vegetable Oils as Fuels

1982." American Society of Agricultural Engineers, p. 117-122.

3. Haadad FR, Keating RW and Low GK.C. J. Liquid Chromatography 5.

853 (1982). •

4. McN air HM cmd Bonelli EJ in "Basic Gas Chromatography" Varian

Instrument p. 33. • s. Fedeli E . .:r Aces 60, 404·(1983).

•

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89

GCPLATE PROGRAM

10 REM GO PARAMETERS 20 NEW ~0 PRINT "COLUMN = ?": INPUT B$ 40 PRINT "OPERATOR = ?": INPUT C$ 50 PRINT "TIME TO START CALCULATION?": INPUT TS 60 70 80

PRINT "SAMPLE = ?": INPUT A$ PRINT "FLOW RATE = ? " : INPUT F REM CHECK GO FOR READINESS

90 A1 = LEVEL 100 110 120 130 140 150 160 170 180 190 200 21 0 220 230 240 250 260 270 280 290

WAIT 10 A2 = LEVEL B = ABS(Al -A2) IF 13< 5 THEN 160 PRINT "BASELINE IS NOT STABLE" GOTO 90 T = ABS(CITP#1-CITP#1 M)

IF T<1 THEN 200 PRINT "TEMPERATURE IS NOT STEADY" GOTO 90 PRINT "READY •••• INJECT SAMPLE AND PUSH START BUTTON" ZERO ' REM QUANTITATION AND CALCULATION OF PLATE NUMBER LPRINT LPRINT LPRINT TAB( 20); "DETECTOR RESPONSE" WAIT START WAIT STOP P = MAXPK IF P = 0 THEN 760

300 REM REPORT 310 LPRINT 320 LPRINT "BIOTECHNOLOGY DIV. SCHOOL OF EMERGY AND MATERIALS KMIT T BKK 10140" 330 LPRINT 340 LPRINT "DATE: .. ;DATE$; II TIME: II ;TIME$, "OPERATOR: JI ;C$ 350 L'PRINT "COLUMN: If ;B$, I "SAMPLE: If ;A$ 360 LPRINT "OVEN:- ";CITP#1;" INJECTOR: ";INJT#1, "ATTENUATION: ";ATTE N(FILE); "X10"'II;.DIRG#1 370 LPRINT "FLOW RATE= ";F 380 LPRINT 390 LPRINT 400 R2 = 0 410 W4 = 0 420 A4 "" 0 430 FOR I = 1 TO P 440 A3 = AREA(!) 450 460 470

A4 = A4 + A3 IF RWRT(I) < TS THEN A4 NEXT I

•

0

89-a

480 LPRINT "PKNO" i II TIME" i II AREA";" HEIGHT";" CONC";" ATE NO";" RESN" 490 FOR I = 1 TO P 500 A3 = AREA(I) 510 H1 = HI (I) 520 W3 = A3/H1 530 R1 = RWRT(I) 540 ea = 100*A3/A4 550 IF RWRT(I)<TS THEN ea = 0) 560 ' N:1 = 22500*R1 .. 2/W3"2 570 R4 = 60*(R1-R2)/(W3+W4) 580 IF I = 1 THEN R4 = 0

LPRINT USING 600;I,RWRT(I),A3,H1 ,C8,N1,R4 590 600 610 620 630 640 650 660 670 680 690 700 710 720 730

IMAGE;>### ##.## >######## >#####f ##.## >##### ##.## R2 = R~T(I) , W4 = W3 NEXT I LPRINT LPRINT PRINT uANYMORE SAMPLE ? (Y/N)": INPUT G$ IF G$ = "N" THEN 740 IF G$ = "Y" THEN 700 GOTO 660 PRINT "NEW SAMPLE NAME ? (Y/N)": INPUT F$ IF F $ = uy" 'l'HEN 60 IF F$ = "N" 'l'HEN 70 GOTO 700

740 PRINT II END" 750 END 760 PRINT "NO PEAK" 770 GOTO 90

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44 1901 1887 1894 ·-

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14 3225 - 3225

10 3163 - 3163

8 2895 - 2895

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DETECTOR RESPONSE

l

10

39 3

4 49

BIOTECHNOLOGY DlY. SCHOOL Or ENERGY AND MATERIALS KMITT BKK 10140

DATE: 001'001'9"8 'TJHE: 91185:38 COLUl1H: 15~ DEGS OVEN: 170 INJECTOR: . 188 rLOU RATE = 14

PKNO TIME AREA HEIGHT 1 1. 92 28212268 1216837 2 23.8 9861 148 3 31. 88 1752 25 4 37 1596 18 5 43.28 246139 . 2101

OPERATOR: KANIT KRISNANGKURA SAMPLE: PALME 5 UL ATTENUATION: 3 X10A 2

CONC PLATE NO RESN e 154 0 3.89 2854 14.59 0.69 4502 3.51 0.63 3888 1.92

94.79 3225 1. 85

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METHYL PAL111TAT£ 3

9 METH'tL STEARATE ~ z 20 METHYL OLEATE:

12

30

CJtROnATOPAC C-R3A SAMPLE HO 9 REPORT HO 1263

11fl./11U J n

METHYL LINOLEATE

F'1 LE lfETHOD

PKl'IO TI 11E AREA MK IDHO COHC HAl1E

3

4

6

7

10

11

12

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7. 943

9.717

19.682

11.55

1'L35

12.717

14.511

4869829 4 ho

114& 22.9stb

8291'3 34.6setc·

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199~.

57.9ue 590 y

26,4SotC 1300 y

31.9sec 2469 y

59 •. hec 15958 y

66.9sec 17.243 161613 y

67 sec 22.483 137529

81.4sec 26. 4.83 2555

72.lsee .... _ .. .,_ ...... _ TOTAL

0.8216

1. 554?

0.8163

9. eu1

8.0112

0.9246,

8.0468

0.2855

2.6971

9,0484

1ee i1B41U' 11

BlOTECHHOLOGY D!V. &CHOOL isr f:NERGY AHD nAr£RIALS KMITT BKK 1

DATE: 86/82/17 TfHEr 1ltl11S~ COLUMNt DEGS 15k ~ . . . . OYEHI 178 lHJttiO~I 188 F'LOll RATE = 19

PKl'IO 1 2 3 4

·s 6 7 8 9

u 11

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Tll1£ 8.38 4.3$ 7.94 9.?t!

te.U 11;55 12. 35 12.72' 14.52 17.24 22.48 26.48

AREA 4869828

11.48 821':1 u

861 1.&99 590

13.:'IO 2469

15958 161613 137529

2555

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H£1GHT t215El48

39 1:!369

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225 2413 1690

35

OPERATOR: KAHIT KRlSHANGKURA $.HPLE• FAME<KIHG> 1 UL ATTtHUATIOHI 2 l<18" 2

COHC a 9.28

U.19 e.u e.2s a.1s 9.32 9.61 3.71

39.79 33.86

0,63

PLATE HO 294 825

1185 1368. 765

430?. 3377 1447 1959 1 ,, 1

1717 3039

RESH 9 a. 92 3.72 1.44 e. 59 9.62 0.82

. 9. 27 Q, 92 1.22 2.12 1. 56

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