How they workAbsorption enhancements

Dermoelectroporation® provides..Dermoelectroporation Technology provides the following:

• Skin Bio Revitalization treatments

Existing Technologies

MicrodermabrasionMedical and Aesthetic application

– 1st transdermal pathway– Small molecules allowed to pass– Low delivery rate (twice topical)– Effects limited to the skin surface– No current applied– For use on human beings

Existing TechnologiesTraditional Pulsed Ionthophoresis (1to 5mA)Medical and Aesthetic application

– Only small molecules allowed– Ineffective for high weight molecules– Low AC current level applied– Changes the pH of ionic drug solutions– Electrolysis of the ionic solution– For use on human beings

Low AC current level applied

Existing TechnologiesTraditional Galvanic Ionthophoresis (1to 5mA)Medical and Aesthetic application

– Only small molecules allowed– No high weight molecules delivered– Low DC current level applied– Changes the pH of ionic drug solutions– Electrolysis of the ionic solution– For use on human beings

Low DC current level applied

Existing TechnologiesElectroporation (higher than 5mA)In vitro application only

– High peak currents to “porate” the stratum corneum – High risk of skin damage– Big sized molecules deliverable– Adverse effects– For in lab test only not to use on human beings

High peak current level applied

Existing TechnologiesAquaphoresys (1to 5mA)Medical and Aesthetic application

– Only small molecules allowed– Ineffective for high weight molecules– Ultrasounds and current applied– Changes the pH of ionic drug solutions– Electrolysis of the ionic solution– For use on human beings

Low AC current level applied

Existing Technologies

Ultrasounds Medical and Aesthetic application

– No current applied – High vibration frequency on the skin– Infra-cellular spaces involved– Small molecules allowed to pass– Low delivery rate (twice topical)– Effects limited to the skin surface– For use on human beings

Existing Technologies

Sonphoresys Medical and Aesthetic application

– No current applied – Sonic waveforms the skin– Infra-cellular spaces involved– Small molecules allowed to pass– Low delivery rate (twice topical)– Effects limited to the skin surface– For use on human beings

Existing Technologies

Micro-currents (0 to .5mA) Aesthetic application only

– Very small current applied – Very Low delivery rate– Effects limited to the skin surface– For use on human subjects

Existing Technologies

Cosmetic absorption enhancers (.5 to 1mA) Aesthetic application only

– Very small current applied – Low/mid delivery rate – Effects limited to the skin surface– Infra-cellular spaces involved– Small molecules allowed to pass– For use on human beings

Existing Technologies

Topical Patches Local Aesthetic application only

– Very low current applied – Very Low delivery rate (takes more time)– Effects limited to the skin surface– Some adverse effect reported– For use on human beings

Innovation in transdermal “drug” Delivery

Why we say Innovation?

3 big problems not solved yet with old technologies:

1) Transdermal delivery of macromolecules without side effects 2) Irritation associated to long treatments sessions3) Efficacy in the long term

Engineering of Florence - Italy developed a new methodology using the new Dermoelectroporation technology part of the Transdermal Delivery procedure that eliminates those problems.

Dermoelectroporation® Solution

Dermoelectroporation® removes the limitations…

combines the advantages of the existing technologies

to create the COLLAGENIZER® system

Latest innovation in transdermal Collagen

delivery

How we solve problems ?

1) Transdermal delivery of macromolecules without side effects Collagenizer® can deliver either micro and macro molecules using

low current as proven by scientific publications, clinical reports, studies and doctors using the machine worldwide.

2) Irritation associated to long treatments session Collagenizer® can deliver any type of drugs with no additional return electrode to avoid discomforts

3) Efficacy in the long term Collagenizer® is used by professional users getting astonishing results instantly, mid and long term.

Dermoelectroporation® means…• DERMOELECTROPORATION® means:

– Dermo = From preventive MicroDERMoabrasion.

– Electroporation = From the shape of the electrical pulses that are instead lowered in current - max 1mA- delivered on the dermis after microdermabrasion. These pulses that are similar in shape to the electroporation, are instead, completely different from those in value, time duration, electrical characteristics.

Dermoelectroporation® features• Dermoelectroporation technology ……

– utilizes the skin’s water based “channels” – Controlled “electroporation-like” continuous reversed polarity

electrical pulses (Average current value on the patient is zero).– Increase the skin permeability to allow transdermal delivery of

micro-molecules and macro-molecules – No modification of the ionic drug solutions pH and no

electrolysis effect of the ionic solution itself. –  Dermoelectroporation takes less time vs traditional

Ionthophoresis devices to deliver the same amount of ionic drug solution.

– Dermoelectroporation does not need any skin pH-Compensating patch because does not causes irritation on the skin as Ionthophoresis devices do.

Dermoelectroporation® …Unique features: • Allow to deliver macromolecules (greater than

800.000d)• Positive and negative ions of the drug are delivered at

the same time • Do not cause Electrolysis neither change the drug pH• Do not irritate nor make any damage to the skin.• Do not need return electrode, conductive gels, pre

charged compounds, pH compensating patches.• Allow to determine the dosage to administer.• Allow the reproducibility of the treatment.

Dermoelectroporation® closeup…

Skin

Water based channels

Molecules ions

Results of Dermoelectroporation®

Transdermal delivery of:Ionic solution of low-weight molecules

More effective than traditional systems

Transdermal delivery feature not available for patients with other techniques

Advantages of Dermoelectroporation®

Courtesy of Dr. Jaggy Rao, MD University of Edmonton ,Alberta,Canada

Before!

Courtesy of Dr. Jaggy Rao, MD University of Edmonton ,Alberta,Canada

After!