abs viola tricolor extract - active...
TRANSCRIPT
ABS Viola Tricolor Extract
Tomorrowrsquos Visionhellip Todayreg
Hydration + Hyaluronic Acid Synthesis + Anti-Wrinkle
ABS Viola Tricolor Extract
Technical Information
Product Code 10346
INCI Name Hydrolyzed Viola Tricolor Extract
INCI Status Approved
Suggested Use Level 20 ndash 40
Suggested Applications Hydration Hyaluronic Acid Synthesis Anti-Wrinkle
Viola Tricolor Wild Pansy
bull Perennial herb with tricolored flowers
bull Considered beneficial in disease of the heart ndash often called ldquoHeartseaserdquo
bull Traditional herbal remedybull Anti-inflammatory properties
bull Viola tricolor contains- Flavonoids- Tocopherol- Tannins
bull Modulates aquaporin activity bull Increases the synthesis of Aquaporin-3
o Involved in skin hydration elasticity barrier function and wound healing
bull Water crosses cell membranes in 2 waysbull Diffusion through the lipid bilayerbull Through water channels called aquaporins
bull Aquaporinsbull Membrane pore proteinsbull Function as channels to increase permeability of waterbull Additional mechanism of water transport (besides osmosis) bull Synthesized by human keratinocytes -means to enhance the
moisture of the skin ndash increases osmosis between the dermis amp stratum corneum
ABS Viola Tricolor Extract
bull Natural Active Botanical
bull Rich in Polyscaccharidesbull Strong water holding capacitybull Improves epidermal moisture
bull Functions- Powerful Hydration- Anti-Aging- Anti-Wrinkle- Hyaluronic Acid Synthesis
ABS Viola Tricolor Extract What is it
bull Increases synthesis of Aquaproins-3
bull Increases synthesis of Hyaluronic Acid (HA)
bull Increases proliferation of receptor sites (CD22) that bind HA
bull Increases production of protease (Hyaluronan Synthase-2) necessary for HA production
ABS Viola Tricolor Extract How does it work
Efficacy Data
bull In-vivo Increase in Hydration Increase in Long Term Hydration Changes in Wrinkle Characteristics
bull In-vitro Aquaporin-3 Synthesis Increase in CD44 Receptor Sites Hyaluronic Acid Production Production of Hyaluronin Synthase-2
Protocol
bull Equipment Corneometer
bull Principle of measurement Frequency probe -measured electric capacitance of the skin based on the ability of intercellular water levels of the stratum corneum to conduct electrons
bull Subjects 25 (f)
bull Test area Calf region of leg
Figure 1 Hydration Results Comparing 4 ABS Viola Tricolor Extract and Placebo
Increase in Hydration
In- vivo Efficacy ABS Viola Tricolor Extract
0
2
4
6
8
10
12
14
16
Mean Increase in Hydration
Perc
ent (
) I
ncre
ase
Lotion containing 4 ABS Viola Tricolor Extract Lotion containing Placebo
Protocol
bull Equipment Corneometer
bull Principle of measurement Frequency probe -measured electric capacitance of the skin based on the ability of intercellular water levels of the stratum corneum to conduct electrons
bull Subjects 25 (f)
bull Test area Calf region of leg zones measured after 14 amp 28 days
Figure 2 Hydration Results Comparing 4 ABS Viola Tricolor Extract and Placebo
Increase in Long Term Hydration
In- vivo Efficacy ABS Viola Tricolor Extract
0
5
10
15
20
25
Day 14 Day 28
Mea
n Pe
rcen
t (
) Inc
reas
e
Placebo 4 ABS Viola Tricolor Extract
Protocol
bull Equipment Silicone Molds
bull Principle of measurement Profilometer equipped with an image analyzer
bull Subjects 25 (f)
bull Test area Outer eye area
Figure 3 Mean changes in wrinkle characteristics using 4 ABS Viola Tricolor Extract
Mean Changes in Wrinkle Characteristics
In- vivo Efficacy ABS Viola Tricolor Extract
-16
-14
-12
-10
-8
-6
-4
-2
0
Perc
ent (
) D
ecre
ase
Number of wrinkles Total wrinkle surface Total length
Figure 4 Aquaporin-3 production comparing ABS Viola Tricolor Extract control and EGF
Aquaporin-3 Synthesis
In- vitro Efficacy ABS Viola Tricolor Extract
0
20
40
60
80
100
120
140
160
180
Control EGF (30ngml) 25 ABS ViolaTricolor Extract
5 ABS ViolaTricolor Extract
Perc
ent (
) I
ncre
ase Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull Image analysis was performed with BIO Profil Software
bull The visible bands were than quantified using densitometry
Figure 5 Results of the increase in CD44 following treatment with test materials
Increase in CD44 Receptor Sites
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Immunolabeling of human keratinocytes was performed after a 72-hour incubation period
bull Keratinocytes were then incubated for an additional 45 minutes 4C with the anti-CD44 immunoglobulin
bull A second antibody with luminescent flag was used to identify the immunolabeled cells
bull A quantification of florescence was performed on 10 cells
0
20
40
60
80
100
120
140
160
Control EGF 5 ABS ViolaTricolor Extract
1 ABS ViolaTricolor Extract
2 ABS ViolaTricolor Extract
Perc
ent I
ncre
ase
Figure 6 Hyaluronic Acid Production comparing ABS Viola Tricolor Extractand Control
Hyaluronic Acid Production
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull The keratinocytes were then treated with ABS Viola Tricolor Extract in a diluted culture medium The cells were then incubated under the same conditions for 72 hours
bull The cultured supernatant was stored at -80C before the assay was performed ndash ELISA kit was used 0
20
40
60
80
100
120
140
160
Control 5 ABS Viola TricolorExtract
1 ABS Viola TricolorExtract
Perc
ent I
ncre
ase
Figure 7 Increase in MRNA comparing 10 ABS Viola Tricolor Extract and Placebo
Increase in mRNA Synthesis
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull The total RNA was then extracted form the cells amp reverse transcriptase was used to obtain the DNA sequence analyzed by PCR
bull Internal standard - β-actin mRNA
bull Band intensity was quantified by image analysis - BIO profil system
0
20
40
60
80
100
120
140
160
180
Perc
ent (
) I
ncre
ase
Control 1 ABS Viola TriColor Extract
ABS Viola Tricolor Extract
Technical Information
Product Code 10346
INCI Name Hydrolyzed Viola Tricolor Extract
INCI Status Approved
Suggested Use Level 20 ndash 40
Suggested Applications Hydration Hyaluronic Acid Synthesis Anti-Wrinkle
ACTIVE CONCEPTS LLC
THANK YOU
For more information ndashVisit our websitewwwactiveconceptsllccom
- Slide Number 1
- Slide Number 2
- Slide Number 3
- Slide Number 4
- Slide Number 5
- Slide Number 6
- Slide Number 7
- Slide Number 8
- Slide Number 9
- Slide Number 10
- Slide Number 11
- Slide Number 12
- Slide Number 13
- Slide Number 14
- Slide Number 15
- Slide Number 16
-
ABS Viola Tricolor Extract
Technical Information
Product Code 10346
INCI Name Hydrolyzed Viola Tricolor Extract
INCI Status Approved
Suggested Use Level 20 ndash 40
Suggested Applications Hydration Hyaluronic Acid Synthesis Anti-Wrinkle
Viola Tricolor Wild Pansy
bull Perennial herb with tricolored flowers
bull Considered beneficial in disease of the heart ndash often called ldquoHeartseaserdquo
bull Traditional herbal remedybull Anti-inflammatory properties
bull Viola tricolor contains- Flavonoids- Tocopherol- Tannins
bull Modulates aquaporin activity bull Increases the synthesis of Aquaporin-3
o Involved in skin hydration elasticity barrier function and wound healing
bull Water crosses cell membranes in 2 waysbull Diffusion through the lipid bilayerbull Through water channels called aquaporins
bull Aquaporinsbull Membrane pore proteinsbull Function as channels to increase permeability of waterbull Additional mechanism of water transport (besides osmosis) bull Synthesized by human keratinocytes -means to enhance the
moisture of the skin ndash increases osmosis between the dermis amp stratum corneum
ABS Viola Tricolor Extract
bull Natural Active Botanical
bull Rich in Polyscaccharidesbull Strong water holding capacitybull Improves epidermal moisture
bull Functions- Powerful Hydration- Anti-Aging- Anti-Wrinkle- Hyaluronic Acid Synthesis
ABS Viola Tricolor Extract What is it
bull Increases synthesis of Aquaproins-3
bull Increases synthesis of Hyaluronic Acid (HA)
bull Increases proliferation of receptor sites (CD22) that bind HA
bull Increases production of protease (Hyaluronan Synthase-2) necessary for HA production
ABS Viola Tricolor Extract How does it work
Efficacy Data
bull In-vivo Increase in Hydration Increase in Long Term Hydration Changes in Wrinkle Characteristics
bull In-vitro Aquaporin-3 Synthesis Increase in CD44 Receptor Sites Hyaluronic Acid Production Production of Hyaluronin Synthase-2
Protocol
bull Equipment Corneometer
bull Principle of measurement Frequency probe -measured electric capacitance of the skin based on the ability of intercellular water levels of the stratum corneum to conduct electrons
bull Subjects 25 (f)
bull Test area Calf region of leg
Figure 1 Hydration Results Comparing 4 ABS Viola Tricolor Extract and Placebo
Increase in Hydration
In- vivo Efficacy ABS Viola Tricolor Extract
0
2
4
6
8
10
12
14
16
Mean Increase in Hydration
Perc
ent (
) I
ncre
ase
Lotion containing 4 ABS Viola Tricolor Extract Lotion containing Placebo
Protocol
bull Equipment Corneometer
bull Principle of measurement Frequency probe -measured electric capacitance of the skin based on the ability of intercellular water levels of the stratum corneum to conduct electrons
bull Subjects 25 (f)
bull Test area Calf region of leg zones measured after 14 amp 28 days
Figure 2 Hydration Results Comparing 4 ABS Viola Tricolor Extract and Placebo
Increase in Long Term Hydration
In- vivo Efficacy ABS Viola Tricolor Extract
0
5
10
15
20
25
Day 14 Day 28
Mea
n Pe
rcen
t (
) Inc
reas
e
Placebo 4 ABS Viola Tricolor Extract
Protocol
bull Equipment Silicone Molds
bull Principle of measurement Profilometer equipped with an image analyzer
bull Subjects 25 (f)
bull Test area Outer eye area
Figure 3 Mean changes in wrinkle characteristics using 4 ABS Viola Tricolor Extract
Mean Changes in Wrinkle Characteristics
In- vivo Efficacy ABS Viola Tricolor Extract
-16
-14
-12
-10
-8
-6
-4
-2
0
Perc
ent (
) D
ecre
ase
Number of wrinkles Total wrinkle surface Total length
Figure 4 Aquaporin-3 production comparing ABS Viola Tricolor Extract control and EGF
Aquaporin-3 Synthesis
In- vitro Efficacy ABS Viola Tricolor Extract
0
20
40
60
80
100
120
140
160
180
Control EGF (30ngml) 25 ABS ViolaTricolor Extract
5 ABS ViolaTricolor Extract
Perc
ent (
) I
ncre
ase Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull Image analysis was performed with BIO Profil Software
bull The visible bands were than quantified using densitometry
Figure 5 Results of the increase in CD44 following treatment with test materials
Increase in CD44 Receptor Sites
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Immunolabeling of human keratinocytes was performed after a 72-hour incubation period
bull Keratinocytes were then incubated for an additional 45 minutes 4C with the anti-CD44 immunoglobulin
bull A second antibody with luminescent flag was used to identify the immunolabeled cells
bull A quantification of florescence was performed on 10 cells
0
20
40
60
80
100
120
140
160
Control EGF 5 ABS ViolaTricolor Extract
1 ABS ViolaTricolor Extract
2 ABS ViolaTricolor Extract
Perc
ent I
ncre
ase
Figure 6 Hyaluronic Acid Production comparing ABS Viola Tricolor Extractand Control
Hyaluronic Acid Production
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull The keratinocytes were then treated with ABS Viola Tricolor Extract in a diluted culture medium The cells were then incubated under the same conditions for 72 hours
bull The cultured supernatant was stored at -80C before the assay was performed ndash ELISA kit was used 0
20
40
60
80
100
120
140
160
Control 5 ABS Viola TricolorExtract
1 ABS Viola TricolorExtract
Perc
ent I
ncre
ase
Figure 7 Increase in MRNA comparing 10 ABS Viola Tricolor Extract and Placebo
Increase in mRNA Synthesis
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull The total RNA was then extracted form the cells amp reverse transcriptase was used to obtain the DNA sequence analyzed by PCR
bull Internal standard - β-actin mRNA
bull Band intensity was quantified by image analysis - BIO profil system
0
20
40
60
80
100
120
140
160
180
Perc
ent (
) I
ncre
ase
Control 1 ABS Viola TriColor Extract
ABS Viola Tricolor Extract
Technical Information
Product Code 10346
INCI Name Hydrolyzed Viola Tricolor Extract
INCI Status Approved
Suggested Use Level 20 ndash 40
Suggested Applications Hydration Hyaluronic Acid Synthesis Anti-Wrinkle
ACTIVE CONCEPTS LLC
THANK YOU
For more information ndashVisit our websitewwwactiveconceptsllccom
- Slide Number 1
- Slide Number 2
- Slide Number 3
- Slide Number 4
- Slide Number 5
- Slide Number 6
- Slide Number 7
- Slide Number 8
- Slide Number 9
- Slide Number 10
- Slide Number 11
- Slide Number 12
- Slide Number 13
- Slide Number 14
- Slide Number 15
- Slide Number 16
-
Viola Tricolor Wild Pansy
bull Perennial herb with tricolored flowers
bull Considered beneficial in disease of the heart ndash often called ldquoHeartseaserdquo
bull Traditional herbal remedybull Anti-inflammatory properties
bull Viola tricolor contains- Flavonoids- Tocopherol- Tannins
bull Modulates aquaporin activity bull Increases the synthesis of Aquaporin-3
o Involved in skin hydration elasticity barrier function and wound healing
bull Water crosses cell membranes in 2 waysbull Diffusion through the lipid bilayerbull Through water channels called aquaporins
bull Aquaporinsbull Membrane pore proteinsbull Function as channels to increase permeability of waterbull Additional mechanism of water transport (besides osmosis) bull Synthesized by human keratinocytes -means to enhance the
moisture of the skin ndash increases osmosis between the dermis amp stratum corneum
ABS Viola Tricolor Extract
bull Natural Active Botanical
bull Rich in Polyscaccharidesbull Strong water holding capacitybull Improves epidermal moisture
bull Functions- Powerful Hydration- Anti-Aging- Anti-Wrinkle- Hyaluronic Acid Synthesis
ABS Viola Tricolor Extract What is it
bull Increases synthesis of Aquaproins-3
bull Increases synthesis of Hyaluronic Acid (HA)
bull Increases proliferation of receptor sites (CD22) that bind HA
bull Increases production of protease (Hyaluronan Synthase-2) necessary for HA production
ABS Viola Tricolor Extract How does it work
Efficacy Data
bull In-vivo Increase in Hydration Increase in Long Term Hydration Changes in Wrinkle Characteristics
bull In-vitro Aquaporin-3 Synthesis Increase in CD44 Receptor Sites Hyaluronic Acid Production Production of Hyaluronin Synthase-2
Protocol
bull Equipment Corneometer
bull Principle of measurement Frequency probe -measured electric capacitance of the skin based on the ability of intercellular water levels of the stratum corneum to conduct electrons
bull Subjects 25 (f)
bull Test area Calf region of leg
Figure 1 Hydration Results Comparing 4 ABS Viola Tricolor Extract and Placebo
Increase in Hydration
In- vivo Efficacy ABS Viola Tricolor Extract
0
2
4
6
8
10
12
14
16
Mean Increase in Hydration
Perc
ent (
) I
ncre
ase
Lotion containing 4 ABS Viola Tricolor Extract Lotion containing Placebo
Protocol
bull Equipment Corneometer
bull Principle of measurement Frequency probe -measured electric capacitance of the skin based on the ability of intercellular water levels of the stratum corneum to conduct electrons
bull Subjects 25 (f)
bull Test area Calf region of leg zones measured after 14 amp 28 days
Figure 2 Hydration Results Comparing 4 ABS Viola Tricolor Extract and Placebo
Increase in Long Term Hydration
In- vivo Efficacy ABS Viola Tricolor Extract
0
5
10
15
20
25
Day 14 Day 28
Mea
n Pe
rcen
t (
) Inc
reas
e
Placebo 4 ABS Viola Tricolor Extract
Protocol
bull Equipment Silicone Molds
bull Principle of measurement Profilometer equipped with an image analyzer
bull Subjects 25 (f)
bull Test area Outer eye area
Figure 3 Mean changes in wrinkle characteristics using 4 ABS Viola Tricolor Extract
Mean Changes in Wrinkle Characteristics
In- vivo Efficacy ABS Viola Tricolor Extract
-16
-14
-12
-10
-8
-6
-4
-2
0
Perc
ent (
) D
ecre
ase
Number of wrinkles Total wrinkle surface Total length
Figure 4 Aquaporin-3 production comparing ABS Viola Tricolor Extract control and EGF
Aquaporin-3 Synthesis
In- vitro Efficacy ABS Viola Tricolor Extract
0
20
40
60
80
100
120
140
160
180
Control EGF (30ngml) 25 ABS ViolaTricolor Extract
5 ABS ViolaTricolor Extract
Perc
ent (
) I
ncre
ase Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull Image analysis was performed with BIO Profil Software
bull The visible bands were than quantified using densitometry
Figure 5 Results of the increase in CD44 following treatment with test materials
Increase in CD44 Receptor Sites
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Immunolabeling of human keratinocytes was performed after a 72-hour incubation period
bull Keratinocytes were then incubated for an additional 45 minutes 4C with the anti-CD44 immunoglobulin
bull A second antibody with luminescent flag was used to identify the immunolabeled cells
bull A quantification of florescence was performed on 10 cells
0
20
40
60
80
100
120
140
160
Control EGF 5 ABS ViolaTricolor Extract
1 ABS ViolaTricolor Extract
2 ABS ViolaTricolor Extract
Perc
ent I
ncre
ase
Figure 6 Hyaluronic Acid Production comparing ABS Viola Tricolor Extractand Control
Hyaluronic Acid Production
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull The keratinocytes were then treated with ABS Viola Tricolor Extract in a diluted culture medium The cells were then incubated under the same conditions for 72 hours
bull The cultured supernatant was stored at -80C before the assay was performed ndash ELISA kit was used 0
20
40
60
80
100
120
140
160
Control 5 ABS Viola TricolorExtract
1 ABS Viola TricolorExtract
Perc
ent I
ncre
ase
Figure 7 Increase in MRNA comparing 10 ABS Viola Tricolor Extract and Placebo
Increase in mRNA Synthesis
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull The total RNA was then extracted form the cells amp reverse transcriptase was used to obtain the DNA sequence analyzed by PCR
bull Internal standard - β-actin mRNA
bull Band intensity was quantified by image analysis - BIO profil system
0
20
40
60
80
100
120
140
160
180
Perc
ent (
) I
ncre
ase
Control 1 ABS Viola TriColor Extract
ABS Viola Tricolor Extract
Technical Information
Product Code 10346
INCI Name Hydrolyzed Viola Tricolor Extract
INCI Status Approved
Suggested Use Level 20 ndash 40
Suggested Applications Hydration Hyaluronic Acid Synthesis Anti-Wrinkle
ACTIVE CONCEPTS LLC
THANK YOU
For more information ndashVisit our websitewwwactiveconceptsllccom
- Slide Number 1
- Slide Number 2
- Slide Number 3
- Slide Number 4
- Slide Number 5
- Slide Number 6
- Slide Number 7
- Slide Number 8
- Slide Number 9
- Slide Number 10
- Slide Number 11
- Slide Number 12
- Slide Number 13
- Slide Number 14
- Slide Number 15
- Slide Number 16
-
bull Modulates aquaporin activity bull Increases the synthesis of Aquaporin-3
o Involved in skin hydration elasticity barrier function and wound healing
bull Water crosses cell membranes in 2 waysbull Diffusion through the lipid bilayerbull Through water channels called aquaporins
bull Aquaporinsbull Membrane pore proteinsbull Function as channels to increase permeability of waterbull Additional mechanism of water transport (besides osmosis) bull Synthesized by human keratinocytes -means to enhance the
moisture of the skin ndash increases osmosis between the dermis amp stratum corneum
ABS Viola Tricolor Extract
bull Natural Active Botanical
bull Rich in Polyscaccharidesbull Strong water holding capacitybull Improves epidermal moisture
bull Functions- Powerful Hydration- Anti-Aging- Anti-Wrinkle- Hyaluronic Acid Synthesis
ABS Viola Tricolor Extract What is it
bull Increases synthesis of Aquaproins-3
bull Increases synthesis of Hyaluronic Acid (HA)
bull Increases proliferation of receptor sites (CD22) that bind HA
bull Increases production of protease (Hyaluronan Synthase-2) necessary for HA production
ABS Viola Tricolor Extract How does it work
Efficacy Data
bull In-vivo Increase in Hydration Increase in Long Term Hydration Changes in Wrinkle Characteristics
bull In-vitro Aquaporin-3 Synthesis Increase in CD44 Receptor Sites Hyaluronic Acid Production Production of Hyaluronin Synthase-2
Protocol
bull Equipment Corneometer
bull Principle of measurement Frequency probe -measured electric capacitance of the skin based on the ability of intercellular water levels of the stratum corneum to conduct electrons
bull Subjects 25 (f)
bull Test area Calf region of leg
Figure 1 Hydration Results Comparing 4 ABS Viola Tricolor Extract and Placebo
Increase in Hydration
In- vivo Efficacy ABS Viola Tricolor Extract
0
2
4
6
8
10
12
14
16
Mean Increase in Hydration
Perc
ent (
) I
ncre
ase
Lotion containing 4 ABS Viola Tricolor Extract Lotion containing Placebo
Protocol
bull Equipment Corneometer
bull Principle of measurement Frequency probe -measured electric capacitance of the skin based on the ability of intercellular water levels of the stratum corneum to conduct electrons
bull Subjects 25 (f)
bull Test area Calf region of leg zones measured after 14 amp 28 days
Figure 2 Hydration Results Comparing 4 ABS Viola Tricolor Extract and Placebo
Increase in Long Term Hydration
In- vivo Efficacy ABS Viola Tricolor Extract
0
5
10
15
20
25
Day 14 Day 28
Mea
n Pe
rcen
t (
) Inc
reas
e
Placebo 4 ABS Viola Tricolor Extract
Protocol
bull Equipment Silicone Molds
bull Principle of measurement Profilometer equipped with an image analyzer
bull Subjects 25 (f)
bull Test area Outer eye area
Figure 3 Mean changes in wrinkle characteristics using 4 ABS Viola Tricolor Extract
Mean Changes in Wrinkle Characteristics
In- vivo Efficacy ABS Viola Tricolor Extract
-16
-14
-12
-10
-8
-6
-4
-2
0
Perc
ent (
) D
ecre
ase
Number of wrinkles Total wrinkle surface Total length
Figure 4 Aquaporin-3 production comparing ABS Viola Tricolor Extract control and EGF
Aquaporin-3 Synthesis
In- vitro Efficacy ABS Viola Tricolor Extract
0
20
40
60
80
100
120
140
160
180
Control EGF (30ngml) 25 ABS ViolaTricolor Extract
5 ABS ViolaTricolor Extract
Perc
ent (
) I
ncre
ase Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull Image analysis was performed with BIO Profil Software
bull The visible bands were than quantified using densitometry
Figure 5 Results of the increase in CD44 following treatment with test materials
Increase in CD44 Receptor Sites
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Immunolabeling of human keratinocytes was performed after a 72-hour incubation period
bull Keratinocytes were then incubated for an additional 45 minutes 4C with the anti-CD44 immunoglobulin
bull A second antibody with luminescent flag was used to identify the immunolabeled cells
bull A quantification of florescence was performed on 10 cells
0
20
40
60
80
100
120
140
160
Control EGF 5 ABS ViolaTricolor Extract
1 ABS ViolaTricolor Extract
2 ABS ViolaTricolor Extract
Perc
ent I
ncre
ase
Figure 6 Hyaluronic Acid Production comparing ABS Viola Tricolor Extractand Control
Hyaluronic Acid Production
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull The keratinocytes were then treated with ABS Viola Tricolor Extract in a diluted culture medium The cells were then incubated under the same conditions for 72 hours
bull The cultured supernatant was stored at -80C before the assay was performed ndash ELISA kit was used 0
20
40
60
80
100
120
140
160
Control 5 ABS Viola TricolorExtract
1 ABS Viola TricolorExtract
Perc
ent I
ncre
ase
Figure 7 Increase in MRNA comparing 10 ABS Viola Tricolor Extract and Placebo
Increase in mRNA Synthesis
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull The total RNA was then extracted form the cells amp reverse transcriptase was used to obtain the DNA sequence analyzed by PCR
bull Internal standard - β-actin mRNA
bull Band intensity was quantified by image analysis - BIO profil system
0
20
40
60
80
100
120
140
160
180
Perc
ent (
) I
ncre
ase
Control 1 ABS Viola TriColor Extract
ABS Viola Tricolor Extract
Technical Information
Product Code 10346
INCI Name Hydrolyzed Viola Tricolor Extract
INCI Status Approved
Suggested Use Level 20 ndash 40
Suggested Applications Hydration Hyaluronic Acid Synthesis Anti-Wrinkle
ACTIVE CONCEPTS LLC
THANK YOU
For more information ndashVisit our websitewwwactiveconceptsllccom
- Slide Number 1
- Slide Number 2
- Slide Number 3
- Slide Number 4
- Slide Number 5
- Slide Number 6
- Slide Number 7
- Slide Number 8
- Slide Number 9
- Slide Number 10
- Slide Number 11
- Slide Number 12
- Slide Number 13
- Slide Number 14
- Slide Number 15
- Slide Number 16
-
bull Natural Active Botanical
bull Rich in Polyscaccharidesbull Strong water holding capacitybull Improves epidermal moisture
bull Functions- Powerful Hydration- Anti-Aging- Anti-Wrinkle- Hyaluronic Acid Synthesis
ABS Viola Tricolor Extract What is it
bull Increases synthesis of Aquaproins-3
bull Increases synthesis of Hyaluronic Acid (HA)
bull Increases proliferation of receptor sites (CD22) that bind HA
bull Increases production of protease (Hyaluronan Synthase-2) necessary for HA production
ABS Viola Tricolor Extract How does it work
Efficacy Data
bull In-vivo Increase in Hydration Increase in Long Term Hydration Changes in Wrinkle Characteristics
bull In-vitro Aquaporin-3 Synthesis Increase in CD44 Receptor Sites Hyaluronic Acid Production Production of Hyaluronin Synthase-2
Protocol
bull Equipment Corneometer
bull Principle of measurement Frequency probe -measured electric capacitance of the skin based on the ability of intercellular water levels of the stratum corneum to conduct electrons
bull Subjects 25 (f)
bull Test area Calf region of leg
Figure 1 Hydration Results Comparing 4 ABS Viola Tricolor Extract and Placebo
Increase in Hydration
In- vivo Efficacy ABS Viola Tricolor Extract
0
2
4
6
8
10
12
14
16
Mean Increase in Hydration
Perc
ent (
) I
ncre
ase
Lotion containing 4 ABS Viola Tricolor Extract Lotion containing Placebo
Protocol
bull Equipment Corneometer
bull Principle of measurement Frequency probe -measured electric capacitance of the skin based on the ability of intercellular water levels of the stratum corneum to conduct electrons
bull Subjects 25 (f)
bull Test area Calf region of leg zones measured after 14 amp 28 days
Figure 2 Hydration Results Comparing 4 ABS Viola Tricolor Extract and Placebo
Increase in Long Term Hydration
In- vivo Efficacy ABS Viola Tricolor Extract
0
5
10
15
20
25
Day 14 Day 28
Mea
n Pe
rcen
t (
) Inc
reas
e
Placebo 4 ABS Viola Tricolor Extract
Protocol
bull Equipment Silicone Molds
bull Principle of measurement Profilometer equipped with an image analyzer
bull Subjects 25 (f)
bull Test area Outer eye area
Figure 3 Mean changes in wrinkle characteristics using 4 ABS Viola Tricolor Extract
Mean Changes in Wrinkle Characteristics
In- vivo Efficacy ABS Viola Tricolor Extract
-16
-14
-12
-10
-8
-6
-4
-2
0
Perc
ent (
) D
ecre
ase
Number of wrinkles Total wrinkle surface Total length
Figure 4 Aquaporin-3 production comparing ABS Viola Tricolor Extract control and EGF
Aquaporin-3 Synthesis
In- vitro Efficacy ABS Viola Tricolor Extract
0
20
40
60
80
100
120
140
160
180
Control EGF (30ngml) 25 ABS ViolaTricolor Extract
5 ABS ViolaTricolor Extract
Perc
ent (
) I
ncre
ase Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull Image analysis was performed with BIO Profil Software
bull The visible bands were than quantified using densitometry
Figure 5 Results of the increase in CD44 following treatment with test materials
Increase in CD44 Receptor Sites
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Immunolabeling of human keratinocytes was performed after a 72-hour incubation period
bull Keratinocytes were then incubated for an additional 45 minutes 4C with the anti-CD44 immunoglobulin
bull A second antibody with luminescent flag was used to identify the immunolabeled cells
bull A quantification of florescence was performed on 10 cells
0
20
40
60
80
100
120
140
160
Control EGF 5 ABS ViolaTricolor Extract
1 ABS ViolaTricolor Extract
2 ABS ViolaTricolor Extract
Perc
ent I
ncre
ase
Figure 6 Hyaluronic Acid Production comparing ABS Viola Tricolor Extractand Control
Hyaluronic Acid Production
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull The keratinocytes were then treated with ABS Viola Tricolor Extract in a diluted culture medium The cells were then incubated under the same conditions for 72 hours
bull The cultured supernatant was stored at -80C before the assay was performed ndash ELISA kit was used 0
20
40
60
80
100
120
140
160
Control 5 ABS Viola TricolorExtract
1 ABS Viola TricolorExtract
Perc
ent I
ncre
ase
Figure 7 Increase in MRNA comparing 10 ABS Viola Tricolor Extract and Placebo
Increase in mRNA Synthesis
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull The total RNA was then extracted form the cells amp reverse transcriptase was used to obtain the DNA sequence analyzed by PCR
bull Internal standard - β-actin mRNA
bull Band intensity was quantified by image analysis - BIO profil system
0
20
40
60
80
100
120
140
160
180
Perc
ent (
) I
ncre
ase
Control 1 ABS Viola TriColor Extract
ABS Viola Tricolor Extract
Technical Information
Product Code 10346
INCI Name Hydrolyzed Viola Tricolor Extract
INCI Status Approved
Suggested Use Level 20 ndash 40
Suggested Applications Hydration Hyaluronic Acid Synthesis Anti-Wrinkle
ACTIVE CONCEPTS LLC
THANK YOU
For more information ndashVisit our websitewwwactiveconceptsllccom
- Slide Number 1
- Slide Number 2
- Slide Number 3
- Slide Number 4
- Slide Number 5
- Slide Number 6
- Slide Number 7
- Slide Number 8
- Slide Number 9
- Slide Number 10
- Slide Number 11
- Slide Number 12
- Slide Number 13
- Slide Number 14
- Slide Number 15
- Slide Number 16
-
bull Increases synthesis of Aquaproins-3
bull Increases synthesis of Hyaluronic Acid (HA)
bull Increases proliferation of receptor sites (CD22) that bind HA
bull Increases production of protease (Hyaluronan Synthase-2) necessary for HA production
ABS Viola Tricolor Extract How does it work
Efficacy Data
bull In-vivo Increase in Hydration Increase in Long Term Hydration Changes in Wrinkle Characteristics
bull In-vitro Aquaporin-3 Synthesis Increase in CD44 Receptor Sites Hyaluronic Acid Production Production of Hyaluronin Synthase-2
Protocol
bull Equipment Corneometer
bull Principle of measurement Frequency probe -measured electric capacitance of the skin based on the ability of intercellular water levels of the stratum corneum to conduct electrons
bull Subjects 25 (f)
bull Test area Calf region of leg
Figure 1 Hydration Results Comparing 4 ABS Viola Tricolor Extract and Placebo
Increase in Hydration
In- vivo Efficacy ABS Viola Tricolor Extract
0
2
4
6
8
10
12
14
16
Mean Increase in Hydration
Perc
ent (
) I
ncre
ase
Lotion containing 4 ABS Viola Tricolor Extract Lotion containing Placebo
Protocol
bull Equipment Corneometer
bull Principle of measurement Frequency probe -measured electric capacitance of the skin based on the ability of intercellular water levels of the stratum corneum to conduct electrons
bull Subjects 25 (f)
bull Test area Calf region of leg zones measured after 14 amp 28 days
Figure 2 Hydration Results Comparing 4 ABS Viola Tricolor Extract and Placebo
Increase in Long Term Hydration
In- vivo Efficacy ABS Viola Tricolor Extract
0
5
10
15
20
25
Day 14 Day 28
Mea
n Pe
rcen
t (
) Inc
reas
e
Placebo 4 ABS Viola Tricolor Extract
Protocol
bull Equipment Silicone Molds
bull Principle of measurement Profilometer equipped with an image analyzer
bull Subjects 25 (f)
bull Test area Outer eye area
Figure 3 Mean changes in wrinkle characteristics using 4 ABS Viola Tricolor Extract
Mean Changes in Wrinkle Characteristics
In- vivo Efficacy ABS Viola Tricolor Extract
-16
-14
-12
-10
-8
-6
-4
-2
0
Perc
ent (
) D
ecre
ase
Number of wrinkles Total wrinkle surface Total length
Figure 4 Aquaporin-3 production comparing ABS Viola Tricolor Extract control and EGF
Aquaporin-3 Synthesis
In- vitro Efficacy ABS Viola Tricolor Extract
0
20
40
60
80
100
120
140
160
180
Control EGF (30ngml) 25 ABS ViolaTricolor Extract
5 ABS ViolaTricolor Extract
Perc
ent (
) I
ncre
ase Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull Image analysis was performed with BIO Profil Software
bull The visible bands were than quantified using densitometry
Figure 5 Results of the increase in CD44 following treatment with test materials
Increase in CD44 Receptor Sites
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Immunolabeling of human keratinocytes was performed after a 72-hour incubation period
bull Keratinocytes were then incubated for an additional 45 minutes 4C with the anti-CD44 immunoglobulin
bull A second antibody with luminescent flag was used to identify the immunolabeled cells
bull A quantification of florescence was performed on 10 cells
0
20
40
60
80
100
120
140
160
Control EGF 5 ABS ViolaTricolor Extract
1 ABS ViolaTricolor Extract
2 ABS ViolaTricolor Extract
Perc
ent I
ncre
ase
Figure 6 Hyaluronic Acid Production comparing ABS Viola Tricolor Extractand Control
Hyaluronic Acid Production
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull The keratinocytes were then treated with ABS Viola Tricolor Extract in a diluted culture medium The cells were then incubated under the same conditions for 72 hours
bull The cultured supernatant was stored at -80C before the assay was performed ndash ELISA kit was used 0
20
40
60
80
100
120
140
160
Control 5 ABS Viola TricolorExtract
1 ABS Viola TricolorExtract
Perc
ent I
ncre
ase
Figure 7 Increase in MRNA comparing 10 ABS Viola Tricolor Extract and Placebo
Increase in mRNA Synthesis
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull The total RNA was then extracted form the cells amp reverse transcriptase was used to obtain the DNA sequence analyzed by PCR
bull Internal standard - β-actin mRNA
bull Band intensity was quantified by image analysis - BIO profil system
0
20
40
60
80
100
120
140
160
180
Perc
ent (
) I
ncre
ase
Control 1 ABS Viola TriColor Extract
ABS Viola Tricolor Extract
Technical Information
Product Code 10346
INCI Name Hydrolyzed Viola Tricolor Extract
INCI Status Approved
Suggested Use Level 20 ndash 40
Suggested Applications Hydration Hyaluronic Acid Synthesis Anti-Wrinkle
ACTIVE CONCEPTS LLC
THANK YOU
For more information ndashVisit our websitewwwactiveconceptsllccom
- Slide Number 1
- Slide Number 2
- Slide Number 3
- Slide Number 4
- Slide Number 5
- Slide Number 6
- Slide Number 7
- Slide Number 8
- Slide Number 9
- Slide Number 10
- Slide Number 11
- Slide Number 12
- Slide Number 13
- Slide Number 14
- Slide Number 15
- Slide Number 16
-
Efficacy Data
bull In-vivo Increase in Hydration Increase in Long Term Hydration Changes in Wrinkle Characteristics
bull In-vitro Aquaporin-3 Synthesis Increase in CD44 Receptor Sites Hyaluronic Acid Production Production of Hyaluronin Synthase-2
Protocol
bull Equipment Corneometer
bull Principle of measurement Frequency probe -measured electric capacitance of the skin based on the ability of intercellular water levels of the stratum corneum to conduct electrons
bull Subjects 25 (f)
bull Test area Calf region of leg
Figure 1 Hydration Results Comparing 4 ABS Viola Tricolor Extract and Placebo
Increase in Hydration
In- vivo Efficacy ABS Viola Tricolor Extract
0
2
4
6
8
10
12
14
16
Mean Increase in Hydration
Perc
ent (
) I
ncre
ase
Lotion containing 4 ABS Viola Tricolor Extract Lotion containing Placebo
Protocol
bull Equipment Corneometer
bull Principle of measurement Frequency probe -measured electric capacitance of the skin based on the ability of intercellular water levels of the stratum corneum to conduct electrons
bull Subjects 25 (f)
bull Test area Calf region of leg zones measured after 14 amp 28 days
Figure 2 Hydration Results Comparing 4 ABS Viola Tricolor Extract and Placebo
Increase in Long Term Hydration
In- vivo Efficacy ABS Viola Tricolor Extract
0
5
10
15
20
25
Day 14 Day 28
Mea
n Pe
rcen
t (
) Inc
reas
e
Placebo 4 ABS Viola Tricolor Extract
Protocol
bull Equipment Silicone Molds
bull Principle of measurement Profilometer equipped with an image analyzer
bull Subjects 25 (f)
bull Test area Outer eye area
Figure 3 Mean changes in wrinkle characteristics using 4 ABS Viola Tricolor Extract
Mean Changes in Wrinkle Characteristics
In- vivo Efficacy ABS Viola Tricolor Extract
-16
-14
-12
-10
-8
-6
-4
-2
0
Perc
ent (
) D
ecre
ase
Number of wrinkles Total wrinkle surface Total length
Figure 4 Aquaporin-3 production comparing ABS Viola Tricolor Extract control and EGF
Aquaporin-3 Synthesis
In- vitro Efficacy ABS Viola Tricolor Extract
0
20
40
60
80
100
120
140
160
180
Control EGF (30ngml) 25 ABS ViolaTricolor Extract
5 ABS ViolaTricolor Extract
Perc
ent (
) I
ncre
ase Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull Image analysis was performed with BIO Profil Software
bull The visible bands were than quantified using densitometry
Figure 5 Results of the increase in CD44 following treatment with test materials
Increase in CD44 Receptor Sites
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Immunolabeling of human keratinocytes was performed after a 72-hour incubation period
bull Keratinocytes were then incubated for an additional 45 minutes 4C with the anti-CD44 immunoglobulin
bull A second antibody with luminescent flag was used to identify the immunolabeled cells
bull A quantification of florescence was performed on 10 cells
0
20
40
60
80
100
120
140
160
Control EGF 5 ABS ViolaTricolor Extract
1 ABS ViolaTricolor Extract
2 ABS ViolaTricolor Extract
Perc
ent I
ncre
ase
Figure 6 Hyaluronic Acid Production comparing ABS Viola Tricolor Extractand Control
Hyaluronic Acid Production
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull The keratinocytes were then treated with ABS Viola Tricolor Extract in a diluted culture medium The cells were then incubated under the same conditions for 72 hours
bull The cultured supernatant was stored at -80C before the assay was performed ndash ELISA kit was used 0
20
40
60
80
100
120
140
160
Control 5 ABS Viola TricolorExtract
1 ABS Viola TricolorExtract
Perc
ent I
ncre
ase
Figure 7 Increase in MRNA comparing 10 ABS Viola Tricolor Extract and Placebo
Increase in mRNA Synthesis
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull The total RNA was then extracted form the cells amp reverse transcriptase was used to obtain the DNA sequence analyzed by PCR
bull Internal standard - β-actin mRNA
bull Band intensity was quantified by image analysis - BIO profil system
0
20
40
60
80
100
120
140
160
180
Perc
ent (
) I
ncre
ase
Control 1 ABS Viola TriColor Extract
ABS Viola Tricolor Extract
Technical Information
Product Code 10346
INCI Name Hydrolyzed Viola Tricolor Extract
INCI Status Approved
Suggested Use Level 20 ndash 40
Suggested Applications Hydration Hyaluronic Acid Synthesis Anti-Wrinkle
ACTIVE CONCEPTS LLC
THANK YOU
For more information ndashVisit our websitewwwactiveconceptsllccom
- Slide Number 1
- Slide Number 2
- Slide Number 3
- Slide Number 4
- Slide Number 5
- Slide Number 6
- Slide Number 7
- Slide Number 8
- Slide Number 9
- Slide Number 10
- Slide Number 11
- Slide Number 12
- Slide Number 13
- Slide Number 14
- Slide Number 15
- Slide Number 16
-
Protocol
bull Equipment Corneometer
bull Principle of measurement Frequency probe -measured electric capacitance of the skin based on the ability of intercellular water levels of the stratum corneum to conduct electrons
bull Subjects 25 (f)
bull Test area Calf region of leg
Figure 1 Hydration Results Comparing 4 ABS Viola Tricolor Extract and Placebo
Increase in Hydration
In- vivo Efficacy ABS Viola Tricolor Extract
0
2
4
6
8
10
12
14
16
Mean Increase in Hydration
Perc
ent (
) I
ncre
ase
Lotion containing 4 ABS Viola Tricolor Extract Lotion containing Placebo
Protocol
bull Equipment Corneometer
bull Principle of measurement Frequency probe -measured electric capacitance of the skin based on the ability of intercellular water levels of the stratum corneum to conduct electrons
bull Subjects 25 (f)
bull Test area Calf region of leg zones measured after 14 amp 28 days
Figure 2 Hydration Results Comparing 4 ABS Viola Tricolor Extract and Placebo
Increase in Long Term Hydration
In- vivo Efficacy ABS Viola Tricolor Extract
0
5
10
15
20
25
Day 14 Day 28
Mea
n Pe
rcen
t (
) Inc
reas
e
Placebo 4 ABS Viola Tricolor Extract
Protocol
bull Equipment Silicone Molds
bull Principle of measurement Profilometer equipped with an image analyzer
bull Subjects 25 (f)
bull Test area Outer eye area
Figure 3 Mean changes in wrinkle characteristics using 4 ABS Viola Tricolor Extract
Mean Changes in Wrinkle Characteristics
In- vivo Efficacy ABS Viola Tricolor Extract
-16
-14
-12
-10
-8
-6
-4
-2
0
Perc
ent (
) D
ecre
ase
Number of wrinkles Total wrinkle surface Total length
Figure 4 Aquaporin-3 production comparing ABS Viola Tricolor Extract control and EGF
Aquaporin-3 Synthesis
In- vitro Efficacy ABS Viola Tricolor Extract
0
20
40
60
80
100
120
140
160
180
Control EGF (30ngml) 25 ABS ViolaTricolor Extract
5 ABS ViolaTricolor Extract
Perc
ent (
) I
ncre
ase Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull Image analysis was performed with BIO Profil Software
bull The visible bands were than quantified using densitometry
Figure 5 Results of the increase in CD44 following treatment with test materials
Increase in CD44 Receptor Sites
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Immunolabeling of human keratinocytes was performed after a 72-hour incubation period
bull Keratinocytes were then incubated for an additional 45 minutes 4C with the anti-CD44 immunoglobulin
bull A second antibody with luminescent flag was used to identify the immunolabeled cells
bull A quantification of florescence was performed on 10 cells
0
20
40
60
80
100
120
140
160
Control EGF 5 ABS ViolaTricolor Extract
1 ABS ViolaTricolor Extract
2 ABS ViolaTricolor Extract
Perc
ent I
ncre
ase
Figure 6 Hyaluronic Acid Production comparing ABS Viola Tricolor Extractand Control
Hyaluronic Acid Production
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull The keratinocytes were then treated with ABS Viola Tricolor Extract in a diluted culture medium The cells were then incubated under the same conditions for 72 hours
bull The cultured supernatant was stored at -80C before the assay was performed ndash ELISA kit was used 0
20
40
60
80
100
120
140
160
Control 5 ABS Viola TricolorExtract
1 ABS Viola TricolorExtract
Perc
ent I
ncre
ase
Figure 7 Increase in MRNA comparing 10 ABS Viola Tricolor Extract and Placebo
Increase in mRNA Synthesis
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull The total RNA was then extracted form the cells amp reverse transcriptase was used to obtain the DNA sequence analyzed by PCR
bull Internal standard - β-actin mRNA
bull Band intensity was quantified by image analysis - BIO profil system
0
20
40
60
80
100
120
140
160
180
Perc
ent (
) I
ncre
ase
Control 1 ABS Viola TriColor Extract
ABS Viola Tricolor Extract
Technical Information
Product Code 10346
INCI Name Hydrolyzed Viola Tricolor Extract
INCI Status Approved
Suggested Use Level 20 ndash 40
Suggested Applications Hydration Hyaluronic Acid Synthesis Anti-Wrinkle
ACTIVE CONCEPTS LLC
THANK YOU
For more information ndashVisit our websitewwwactiveconceptsllccom
- Slide Number 1
- Slide Number 2
- Slide Number 3
- Slide Number 4
- Slide Number 5
- Slide Number 6
- Slide Number 7
- Slide Number 8
- Slide Number 9
- Slide Number 10
- Slide Number 11
- Slide Number 12
- Slide Number 13
- Slide Number 14
- Slide Number 15
- Slide Number 16
-
Protocol
bull Equipment Corneometer
bull Principle of measurement Frequency probe -measured electric capacitance of the skin based on the ability of intercellular water levels of the stratum corneum to conduct electrons
bull Subjects 25 (f)
bull Test area Calf region of leg zones measured after 14 amp 28 days
Figure 2 Hydration Results Comparing 4 ABS Viola Tricolor Extract and Placebo
Increase in Long Term Hydration
In- vivo Efficacy ABS Viola Tricolor Extract
0
5
10
15
20
25
Day 14 Day 28
Mea
n Pe
rcen
t (
) Inc
reas
e
Placebo 4 ABS Viola Tricolor Extract
Protocol
bull Equipment Silicone Molds
bull Principle of measurement Profilometer equipped with an image analyzer
bull Subjects 25 (f)
bull Test area Outer eye area
Figure 3 Mean changes in wrinkle characteristics using 4 ABS Viola Tricolor Extract
Mean Changes in Wrinkle Characteristics
In- vivo Efficacy ABS Viola Tricolor Extract
-16
-14
-12
-10
-8
-6
-4
-2
0
Perc
ent (
) D
ecre
ase
Number of wrinkles Total wrinkle surface Total length
Figure 4 Aquaporin-3 production comparing ABS Viola Tricolor Extract control and EGF
Aquaporin-3 Synthesis
In- vitro Efficacy ABS Viola Tricolor Extract
0
20
40
60
80
100
120
140
160
180
Control EGF (30ngml) 25 ABS ViolaTricolor Extract
5 ABS ViolaTricolor Extract
Perc
ent (
) I
ncre
ase Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull Image analysis was performed with BIO Profil Software
bull The visible bands were than quantified using densitometry
Figure 5 Results of the increase in CD44 following treatment with test materials
Increase in CD44 Receptor Sites
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Immunolabeling of human keratinocytes was performed after a 72-hour incubation period
bull Keratinocytes were then incubated for an additional 45 minutes 4C with the anti-CD44 immunoglobulin
bull A second antibody with luminescent flag was used to identify the immunolabeled cells
bull A quantification of florescence was performed on 10 cells
0
20
40
60
80
100
120
140
160
Control EGF 5 ABS ViolaTricolor Extract
1 ABS ViolaTricolor Extract
2 ABS ViolaTricolor Extract
Perc
ent I
ncre
ase
Figure 6 Hyaluronic Acid Production comparing ABS Viola Tricolor Extractand Control
Hyaluronic Acid Production
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull The keratinocytes were then treated with ABS Viola Tricolor Extract in a diluted culture medium The cells were then incubated under the same conditions for 72 hours
bull The cultured supernatant was stored at -80C before the assay was performed ndash ELISA kit was used 0
20
40
60
80
100
120
140
160
Control 5 ABS Viola TricolorExtract
1 ABS Viola TricolorExtract
Perc
ent I
ncre
ase
Figure 7 Increase in MRNA comparing 10 ABS Viola Tricolor Extract and Placebo
Increase in mRNA Synthesis
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull The total RNA was then extracted form the cells amp reverse transcriptase was used to obtain the DNA sequence analyzed by PCR
bull Internal standard - β-actin mRNA
bull Band intensity was quantified by image analysis - BIO profil system
0
20
40
60
80
100
120
140
160
180
Perc
ent (
) I
ncre
ase
Control 1 ABS Viola TriColor Extract
ABS Viola Tricolor Extract
Technical Information
Product Code 10346
INCI Name Hydrolyzed Viola Tricolor Extract
INCI Status Approved
Suggested Use Level 20 ndash 40
Suggested Applications Hydration Hyaluronic Acid Synthesis Anti-Wrinkle
ACTIVE CONCEPTS LLC
THANK YOU
For more information ndashVisit our websitewwwactiveconceptsllccom
- Slide Number 1
- Slide Number 2
- Slide Number 3
- Slide Number 4
- Slide Number 5
- Slide Number 6
- Slide Number 7
- Slide Number 8
- Slide Number 9
- Slide Number 10
- Slide Number 11
- Slide Number 12
- Slide Number 13
- Slide Number 14
- Slide Number 15
- Slide Number 16
-
Protocol
bull Equipment Silicone Molds
bull Principle of measurement Profilometer equipped with an image analyzer
bull Subjects 25 (f)
bull Test area Outer eye area
Figure 3 Mean changes in wrinkle characteristics using 4 ABS Viola Tricolor Extract
Mean Changes in Wrinkle Characteristics
In- vivo Efficacy ABS Viola Tricolor Extract
-16
-14
-12
-10
-8
-6
-4
-2
0
Perc
ent (
) D
ecre
ase
Number of wrinkles Total wrinkle surface Total length
Figure 4 Aquaporin-3 production comparing ABS Viola Tricolor Extract control and EGF
Aquaporin-3 Synthesis
In- vitro Efficacy ABS Viola Tricolor Extract
0
20
40
60
80
100
120
140
160
180
Control EGF (30ngml) 25 ABS ViolaTricolor Extract
5 ABS ViolaTricolor Extract
Perc
ent (
) I
ncre
ase Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull Image analysis was performed with BIO Profil Software
bull The visible bands were than quantified using densitometry
Figure 5 Results of the increase in CD44 following treatment with test materials
Increase in CD44 Receptor Sites
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Immunolabeling of human keratinocytes was performed after a 72-hour incubation period
bull Keratinocytes were then incubated for an additional 45 minutes 4C with the anti-CD44 immunoglobulin
bull A second antibody with luminescent flag was used to identify the immunolabeled cells
bull A quantification of florescence was performed on 10 cells
0
20
40
60
80
100
120
140
160
Control EGF 5 ABS ViolaTricolor Extract
1 ABS ViolaTricolor Extract
2 ABS ViolaTricolor Extract
Perc
ent I
ncre
ase
Figure 6 Hyaluronic Acid Production comparing ABS Viola Tricolor Extractand Control
Hyaluronic Acid Production
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull The keratinocytes were then treated with ABS Viola Tricolor Extract in a diluted culture medium The cells were then incubated under the same conditions for 72 hours
bull The cultured supernatant was stored at -80C before the assay was performed ndash ELISA kit was used 0
20
40
60
80
100
120
140
160
Control 5 ABS Viola TricolorExtract
1 ABS Viola TricolorExtract
Perc
ent I
ncre
ase
Figure 7 Increase in MRNA comparing 10 ABS Viola Tricolor Extract and Placebo
Increase in mRNA Synthesis
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull The total RNA was then extracted form the cells amp reverse transcriptase was used to obtain the DNA sequence analyzed by PCR
bull Internal standard - β-actin mRNA
bull Band intensity was quantified by image analysis - BIO profil system
0
20
40
60
80
100
120
140
160
180
Perc
ent (
) I
ncre
ase
Control 1 ABS Viola TriColor Extract
ABS Viola Tricolor Extract
Technical Information
Product Code 10346
INCI Name Hydrolyzed Viola Tricolor Extract
INCI Status Approved
Suggested Use Level 20 ndash 40
Suggested Applications Hydration Hyaluronic Acid Synthesis Anti-Wrinkle
ACTIVE CONCEPTS LLC
THANK YOU
For more information ndashVisit our websitewwwactiveconceptsllccom
- Slide Number 1
- Slide Number 2
- Slide Number 3
- Slide Number 4
- Slide Number 5
- Slide Number 6
- Slide Number 7
- Slide Number 8
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Figure 4 Aquaporin-3 production comparing ABS Viola Tricolor Extract control and EGF
Aquaporin-3 Synthesis
In- vitro Efficacy ABS Viola Tricolor Extract
0
20
40
60
80
100
120
140
160
180
Control EGF (30ngml) 25 ABS ViolaTricolor Extract
5 ABS ViolaTricolor Extract
Perc
ent (
) I
ncre
ase Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull Image analysis was performed with BIO Profil Software
bull The visible bands were than quantified using densitometry
Figure 5 Results of the increase in CD44 following treatment with test materials
Increase in CD44 Receptor Sites
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Immunolabeling of human keratinocytes was performed after a 72-hour incubation period
bull Keratinocytes were then incubated for an additional 45 minutes 4C with the anti-CD44 immunoglobulin
bull A second antibody with luminescent flag was used to identify the immunolabeled cells
bull A quantification of florescence was performed on 10 cells
0
20
40
60
80
100
120
140
160
Control EGF 5 ABS ViolaTricolor Extract
1 ABS ViolaTricolor Extract
2 ABS ViolaTricolor Extract
Perc
ent I
ncre
ase
Figure 6 Hyaluronic Acid Production comparing ABS Viola Tricolor Extractand Control
Hyaluronic Acid Production
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull The keratinocytes were then treated with ABS Viola Tricolor Extract in a diluted culture medium The cells were then incubated under the same conditions for 72 hours
bull The cultured supernatant was stored at -80C before the assay was performed ndash ELISA kit was used 0
20
40
60
80
100
120
140
160
Control 5 ABS Viola TricolorExtract
1 ABS Viola TricolorExtract
Perc
ent I
ncre
ase
Figure 7 Increase in MRNA comparing 10 ABS Viola Tricolor Extract and Placebo
Increase in mRNA Synthesis
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull The total RNA was then extracted form the cells amp reverse transcriptase was used to obtain the DNA sequence analyzed by PCR
bull Internal standard - β-actin mRNA
bull Band intensity was quantified by image analysis - BIO profil system
0
20
40
60
80
100
120
140
160
180
Perc
ent (
) I
ncre
ase
Control 1 ABS Viola TriColor Extract
ABS Viola Tricolor Extract
Technical Information
Product Code 10346
INCI Name Hydrolyzed Viola Tricolor Extract
INCI Status Approved
Suggested Use Level 20 ndash 40
Suggested Applications Hydration Hyaluronic Acid Synthesis Anti-Wrinkle
ACTIVE CONCEPTS LLC
THANK YOU
For more information ndashVisit our websitewwwactiveconceptsllccom
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- Slide Number 16
-
Figure 5 Results of the increase in CD44 following treatment with test materials
Increase in CD44 Receptor Sites
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Immunolabeling of human keratinocytes was performed after a 72-hour incubation period
bull Keratinocytes were then incubated for an additional 45 minutes 4C with the anti-CD44 immunoglobulin
bull A second antibody with luminescent flag was used to identify the immunolabeled cells
bull A quantification of florescence was performed on 10 cells
0
20
40
60
80
100
120
140
160
Control EGF 5 ABS ViolaTricolor Extract
1 ABS ViolaTricolor Extract
2 ABS ViolaTricolor Extract
Perc
ent I
ncre
ase
Figure 6 Hyaluronic Acid Production comparing ABS Viola Tricolor Extractand Control
Hyaluronic Acid Production
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull The keratinocytes were then treated with ABS Viola Tricolor Extract in a diluted culture medium The cells were then incubated under the same conditions for 72 hours
bull The cultured supernatant was stored at -80C before the assay was performed ndash ELISA kit was used 0
20
40
60
80
100
120
140
160
Control 5 ABS Viola TricolorExtract
1 ABS Viola TricolorExtract
Perc
ent I
ncre
ase
Figure 7 Increase in MRNA comparing 10 ABS Viola Tricolor Extract and Placebo
Increase in mRNA Synthesis
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull The total RNA was then extracted form the cells amp reverse transcriptase was used to obtain the DNA sequence analyzed by PCR
bull Internal standard - β-actin mRNA
bull Band intensity was quantified by image analysis - BIO profil system
0
20
40
60
80
100
120
140
160
180
Perc
ent (
) I
ncre
ase
Control 1 ABS Viola TriColor Extract
ABS Viola Tricolor Extract
Technical Information
Product Code 10346
INCI Name Hydrolyzed Viola Tricolor Extract
INCI Status Approved
Suggested Use Level 20 ndash 40
Suggested Applications Hydration Hyaluronic Acid Synthesis Anti-Wrinkle
ACTIVE CONCEPTS LLC
THANK YOU
For more information ndashVisit our websitewwwactiveconceptsllccom
- Slide Number 1
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- Slide Number 16
-
Figure 6 Hyaluronic Acid Production comparing ABS Viola Tricolor Extractand Control
Hyaluronic Acid Production
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull The keratinocytes were then treated with ABS Viola Tricolor Extract in a diluted culture medium The cells were then incubated under the same conditions for 72 hours
bull The cultured supernatant was stored at -80C before the assay was performed ndash ELISA kit was used 0
20
40
60
80
100
120
140
160
Control 5 ABS Viola TricolorExtract
1 ABS Viola TricolorExtract
Perc
ent I
ncre
ase
Figure 7 Increase in MRNA comparing 10 ABS Viola Tricolor Extract and Placebo
Increase in mRNA Synthesis
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull The total RNA was then extracted form the cells amp reverse transcriptase was used to obtain the DNA sequence analyzed by PCR
bull Internal standard - β-actin mRNA
bull Band intensity was quantified by image analysis - BIO profil system
0
20
40
60
80
100
120
140
160
180
Perc
ent (
) I
ncre
ase
Control 1 ABS Viola TriColor Extract
ABS Viola Tricolor Extract
Technical Information
Product Code 10346
INCI Name Hydrolyzed Viola Tricolor Extract
INCI Status Approved
Suggested Use Level 20 ndash 40
Suggested Applications Hydration Hyaluronic Acid Synthesis Anti-Wrinkle
ACTIVE CONCEPTS LLC
THANK YOU
For more information ndashVisit our websitewwwactiveconceptsllccom
- Slide Number 1
- Slide Number 2
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- Slide Number 12
- Slide Number 13
- Slide Number 14
- Slide Number 15
- Slide Number 16
-
Figure 7 Increase in MRNA comparing 10 ABS Viola Tricolor Extract and Placebo
Increase in mRNA Synthesis
In- vitro Efficacy ABS Viola Tricolor Extract
Protocol
bull Human keratinocytes were cultured amp in KSFM medium amp incubated for 24 hours 37C with CO2 levels limited to 50
bull The total RNA was then extracted form the cells amp reverse transcriptase was used to obtain the DNA sequence analyzed by PCR
bull Internal standard - β-actin mRNA
bull Band intensity was quantified by image analysis - BIO profil system
0
20
40
60
80
100
120
140
160
180
Perc
ent (
) I
ncre
ase
Control 1 ABS Viola TriColor Extract
ABS Viola Tricolor Extract
Technical Information
Product Code 10346
INCI Name Hydrolyzed Viola Tricolor Extract
INCI Status Approved
Suggested Use Level 20 ndash 40
Suggested Applications Hydration Hyaluronic Acid Synthesis Anti-Wrinkle
ACTIVE CONCEPTS LLC
THANK YOU
For more information ndashVisit our websitewwwactiveconceptsllccom
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- Slide Number 2
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- Slide Number 16
-
ABS Viola Tricolor Extract
Technical Information
Product Code 10346
INCI Name Hydrolyzed Viola Tricolor Extract
INCI Status Approved
Suggested Use Level 20 ndash 40
Suggested Applications Hydration Hyaluronic Acid Synthesis Anti-Wrinkle
ACTIVE CONCEPTS LLC
THANK YOU
For more information ndashVisit our websitewwwactiveconceptsllccom
- Slide Number 1
- Slide Number 2
- Slide Number 3
- Slide Number 4
- Slide Number 5
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-
ACTIVE CONCEPTS LLC
THANK YOU
For more information ndashVisit our websitewwwactiveconceptsllccom
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- Slide Number 2
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