a wild-type pmir-hsur4genesdev.cshlp.org/content/suppl/2015/07/28/29.14... · 7/28/2015 ·...

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Xie_FigS4 α-Int9 DAPI - - primary antibody α-Drosha α-Rabbit-594 A C B mut miR 3’box wild-type - α-FLAG - α-FLAG I I S E S E S E S E FLAG-Int11 E203Q pmiR-HSUR4 antibody Y Y Y Y Y Y Y Y ligation RCA protein B protein A primary antibodies DNA circle secondary antibodies conjugated with DNA oligo ﬂuorescent oligo detection site

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Xie_FigS4

α-Int9

DAPI

--primary antibody α-Drosha

α-Rabbit-594

A

C

B

mut miR 3’box wild-type

- α-FLAG - α-FLAG

I IS E S E S E S E

FLAG-Int11 E203Q

pmiR-HSUR4

antibody

Y YYYY YY

Yligation

RCA

protein B

protein A

primary antibodies

DNA circle

secondary antibodiesconjugated with DNA oligo

�uorescent oligo detection site

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