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  • A Simplified Microscopic Particulate Analysis for use in GARP

    Determination 


    Peter Wallis1, Chelton van Geloven2, Dave Tamblyn3 and Catherine Henry4 1. Hyperion Research Ltd., 1008 Allowance Ave. SE, Medicine Hat, AB T1A 3G8 2. Ministry of Forests, Lands and Natural Resource Operations, 499 George St., Prince George, BC V2L 1R5 3. Northern Health, 299 Victoria St., Prince George, BC V2L 5B8 4. Catherine Henry Environmental Consulting, 2017 Willowview Dr., Dawson Creek, BC V1G 2S6

  • BACKGROUND: Filtering More Water Does Not Affect Risk Prediction

  • MPA-1623: Better Filtration & Elution Technology Saves Field Time and Improves Results

    Low Risk

    Medium Risk

    High Risk

  • Project Objectives

    Test the idea that MPA risk can be estimated from a small, grab sample at Level 2 in a GARP determination Evaluate the contribution of turbidity to risk Can MPA risk be correlated with geochemical measurements in the field or bacteriology?

  • Results from Wells & Springs

    Springs Wells Predicted Risk Developed Undeveloped Drilled Wells Dug Wells Low 5 9 1* 0

    Medium 6 4 8 0

    High 9 2 66 2

    * Hair, probably rodent, was found in this well!

  • What we See Under the Microscope: - Iron, Silica and Clay Minerals

  • - Diatoms

  • - Other Algae

  • - Other Interesting Objects

  • - Plant Debris

  • - Rotifers

    Rotifer https://youtu.be/eVyTJdFifEI

    https://youtu.be/eVyTJdFifEI

  • Turbidity is not closely related to Total Coliforms, E. coli, TOC or TSS

    Es ch

    er ic

    hi a

    co li

    (M PN

    /1 00

    m L)

    0

    125

    250

    375

    500

    Field Turbidity (NTU)

    2 5 8 10 13

    High Medium Low

    Risk Prediction

    To ta

    l C ol

    ifo rm

    s (M

    PN /1

    00 m

    L)

    0

    750

    1500

    2250

    3000

    Field Turbidity (NTU)

    2 5 8 10 13

    High Medium Low

    Risk Prediction

    To ta

    l S us

    pe nd

    ed S

    ol id

    s (m

    g/ L)

    0

    5.5

    11

    16.5

    22

    Field Turbidity (NTU)

    0 20 40 60 80

    High Medium Low

    Risk Prediction

    To ta

    l O rg

    an ic

    C ar

    bo n

    (m g/

    L)

    0

    2

    4

    6

    8

    Field Turbidity (NTU)

    0 20 40 60 80

    High Medium Low

    Risk Prediction

  • But Any E. coli or High Total Coliforms Were Associated with Higher Risk

    Es ch

    er ic

    hi a

    co li

    (M PN

    /1 00

    m L)

    0

    125

    250

    375

    500

    Total Coliforms (MPN/100 mL)

    0 625 1250 1875 2500

    High Medium Low

  • Turbidity and Redox Potential (ORP) are Associated with Risk

    R ed

    ox (

    m V

    )

    -180

    -60

    60

    180

    300

    Turbidity (NTU)

    2 5 8 10 13

    High Medium Low

    2 variable model: Risk = f (Turb, ORP) Risk = m₁ · Turb + m₂ · ORP + ε Turb ORP

    slope (m) 0.022 0.0007 SE(m) 0.009 0.0006 t = m / SE 2.41 1.19 p-value 0.009 0.118 statistical significance ** ns coeff of determination (r²) 6.6%

  • Conclusions

    If surface water organisms can be detected in 2L of water, it’s GARP Filtering large volumes of water does not make an MPA test more sensitive Turbidity and TSS alone are not good GARP indicators unless combined with ORP Wells with positive ORP, especially springs, are more likely to contain surface water organisms

  • Questions?

    www.hyperionlab.ca