a model of the quorum sensing system in genetically engineered

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A Model of the Quorum Sensing System in Genetically Engineered E.coli Using Membrane Computing Afshin Esmaeili, Iman Yazdanbod, Christian Jacob University of Calgary, Calgary, Alberta, Canada Abstract In this article, we present a novel model of a synthetic autoinducer-2 signaling system in ge- netically engineered Escherichia coli (E. coli) bacteria using the recently proposed Membrane Computing (MC) framework [6]. Bacte ria release, receiv e and recogniz e signa ling molecul es in their environment. Many species of bacteria use the information obtained to coordinate their gene expression in response to the size of their population, which is known as Quorum Sensing [reference]. Membrane computing is inspired by the structures and functions of biological membranes. Recently, the MC approach has been used for modeling features of cells in biological systems [6]. A Membrane Compu ting model allows us to observe the behavi or of each indiv idual cell as well as the emer gent proper ties of the whole populati on. It also enables us to manipulat e parameters of the model and to observe and analyze their e ff ects in the simulation. We provide a Membrane Computing model of Quorum Sensing in E. coli which is dened in terms of spatial compartments and interactions among membranes and molecules. For this pur- pose, we extended the original Membrane Computing formalism to accommodate cell division as well as multiple indepen dent computa tiona l units or cells. We expl ain the anal ogy between the computational and the biological model and show the emergent impact of local interactions on the bacterial colony. Keywords: Quorum Sensing, Membrane Computing, Biological Modeling 1. Quorum Sensing Signaling System in Genetic ally Engineered E. coli Bacte ria Bacteria communicate with each other using chemical signaling molecules. The process of producing, releasing, detecting, and responding to the signaling molecules in bacteria is referred to as Quorum Sensing. Thus, Quorum Sensing is a method of intercellular communication which allows populations of bacteria to work together. The relea sed sign aling moleculesare called autoi nduc ers. The conce ntrat ions of autoi nduce rs increase as the bacteria population density grows. The bacteria react to a minimal concentration of an autoinducer (accumulation threshold) as it inuences their gene regulation pathways. Ac- cordingly, due a population-wide signal di ff usion, the bacteria in a community concurrently alter their behavior . Hence, they function similar to a multicellula r organism. Quorum Sensing was disco vere d in the biolumin escent marin e bacte rium Vibr io sch eri. The insights gained from studying the signaling mechanisms involved in Quorum Sensing may be used in various clinical and industrial applications [3]. Preprint submitted to iGEM competition 2009 October 21, 2009

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