a biology and engineering cooperative project james w. stevens m. karen newell university of...
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![Page 1: A Biology and Engineering Cooperative Project James W. Stevens M. Karen Newell University of Colorado at Colorado Springs 30 April 2005](https://reader030.vdocuments.mx/reader030/viewer/2022032521/56649d5e5503460f94a3d15b/html5/thumbnails/1.jpg)
A Biology and A Biology and Engineering Engineering
Cooperative ProjectCooperative ProjectJames W. StevensJames W. StevensM. Karen NewellM. Karen Newell
University of Colorado at Colorado University of Colorado at Colorado SpringsSprings
30 April 200530 April 2005
![Page 2: A Biology and Engineering Cooperative Project James W. Stevens M. Karen Newell University of Colorado at Colorado Springs 30 April 2005](https://reader030.vdocuments.mx/reader030/viewer/2022032521/56649d5e5503460f94a3d15b/html5/thumbnails/2.jpg)
general backgroundgeneral background
connection between cellular connection between cellular metabolic pathways and immune metabolic pathways and immune system responsesystem response
surface signals tied to mitochondrial surface signals tied to mitochondrial activity and fuelactivity and fuel
![Page 3: A Biology and Engineering Cooperative Project James W. Stevens M. Karen Newell University of Colorado at Colorado Springs 30 April 2005](https://reader030.vdocuments.mx/reader030/viewer/2022032521/56649d5e5503460f94a3d15b/html5/thumbnails/3.jpg)
cooperative effortcooperative effort
biologybiology– science driverscience driver– cell culturescell cultures
growinggrowing conditionsconditions
– cell counting and cell counting and physical physical preparationpreparation
engineeringengineering– data acquisitiondata acquisition– temperature temperature
measurementsmeasurements– data reductiondata reduction– data smoothingdata smoothing
![Page 4: A Biology and Engineering Cooperative Project James W. Stevens M. Karen Newell University of Colorado at Colorado Springs 30 April 2005](https://reader030.vdocuments.mx/reader030/viewer/2022032521/56649d5e5503460f94a3d15b/html5/thumbnails/4.jpg)
background-this projectbackground-this project
2 mouse leukemia lines2 mouse leukemia linesL1210 – not drug resistantL1210 – not drug resistant
L1210/ddp– drug resistantL1210/ddp– drug resistant cultured to 10^6 to 10^8 cellscultured to 10^6 to 10^8 cells stressed with bleachstressed with bleach
– measurable, repeatable differencesmeasurable, repeatable differences– different culture conditions, metabolic different culture conditions, metabolic
statesstates
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cumulative heat release
-4
-2
0
2
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10
12
14
16:51:36 16:52:19 16:53:02 16:53:46 16:54:29 16:55:12 16:55:55
time
jou
les
L1210ddp L1210
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dt
dEWQG
)( TThAdt
dTmcG
data analysisdata analysis
first law of thermodynamics:first law of thermodynamics:
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measured heat generation rate
-2
-1
0
1
2
3
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16:52:05 16:52:10 16:52:14 16:52:18 16:52:23 16:52:27 16:52:31
time
wat
ts
L1210ddp L1210
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0 0.2 0.4 0.6 0.8 10
0.5
1
*
*
*)(dt
bt
ec
aety
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data smoothingdata smoothing
least square curve fit:least square curve fit:
minimize minimize
2ˆ*)( iyty
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measured heat generation rate
-2
-1
0
1
2
3
4
5
6
16:52:05 16:52:10 16:52:14 16:52:18 16:52:23 16:52:27 16:52:31
time
wat
ts
L1210ddp, smoothed L1210, smoothed L1210ddp L1210
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summarysummary
leverage complementary capacities– leverage complementary capacities– specific to people and projectspecific to people and project
involve studentsinvolve students
plunge into a new areaplunge into a new area
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other cooperative efforts at UCCSother cooperative efforts at UCCS
biomechanics –biology, mechanical biomechanics –biology, mechanical engineeringengineering
orthopedic mechanics –MERIC, orthopedic mechanics –MERIC, biology, mechanical engineeringbiology, mechanical engineering
feedback controls –MAE, biologyfeedback controls –MAE, biology microgravity – MAEmicrogravity – MAE assistive technologies – MATH, assistive technologies – MATH,
biologybiology