(95368302) cbse xii chemistry project determination of caffeine in tea samples

8/14/2019 (95368302) CBSE XII Chemistry Project Determination of Caffeine in Tea Samples

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Know How much

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caffeine you ar e t ak ing in with eachcup of t ea!

Pr oject PreparedBy:

Ank it BahugunaXII -Ao!! " um #er :

$o%e!y Pu#!ic &r' &ec' &choo! (P')'* ihar +

)etermination of ,affeine in ea &amp!e.Ankit Bahuguna (XII-A+






/

0ir.t of a!! I wou!d !ike to thank my cou.in #rotherMr. Neeraj Naithani e.earch &cho!ar )epartmentof ,hemi.try II oorkee for hi. contri#ution in thi..tudy' I wou!d a!.o !ike to thank my ,hemi.try

eacher Ms. Rampal in pro%iding a he!ping hand inmaking my project tit!ed 2 Determination OfCaffeine In Tea Samples 3'

)i.c!aimer.: he error. idiocie. and incon.i.tencie.remain my own'

Ankit Bahuguna(Name an signature of the stu ent!







4

It i. here#y certify that th eorigina! and genuine r e. ear ch

work i. carried out to in %e. tiga tea#out the .u#ject matter and th ere!ated data co!!ection and h a .

#een comp!eted .o!e!y an d.ati.factori!y #y thi. . tu d en t

regarding the Project tit!ed2Determination Of Caffeine In Tea

Samp les3'






&ign atu r e(&u#ject ea ch er +







5

, 6 " 7 " & 1' In tr odu cti on/' h eor y4' 8.e. of , aff e in e5' 7ffect. of , aff e in e9' Pr ocedu r e' 6 # . er %ati on .;' e . u !t







9

I " 6 ) 8 , I 6 "

ea i. the mo.t common!y and wide!y u . ed.oft #e%erage in the hou.eho!d' It act. a. a.timu!ant for centra! ner%ou. .y.tem an d.ke!eta! mu.c!e.' hat i. why tea r em o%e.fatigue tiredne.. and headache' It a!.oincrea.e. the capacity of thinking' It i. a!.ou.ed for !owering #ody temperature' h e

principa! con.tituent of tea which i.re.pon.i#!e for a!! the.e propertie. i. th ea!ka!oid-caffeine' he amount of caffeine intea !ea%er. %arie. from .amp!e to . am p !e '

6rigina!!y it wa. thought that caffeine i.re.pon.i#!e for the ta.te and f!a%our of tea 'But pure caffeine ha. #een found to #e ata.te!e.. whi!e .u#.tance' herefore th eta.te and f!a%our of tea i. due to .ome o th er.u#.tance pre.ent in it' here i. a !itt !edou#t that the popu!arity of the <an th en e.

#e%erage. depend. on their . tim u !an taction a!though mo.t peop!e are u n aw ar e







of any .timu!ation' he degree to which anindi%idua! i. .timu!ated #y gi%en amount ofcaffeine %arie. from indi%idua! toin d i%idu a!'

0or e<amp!e .ome peop!e #oa.t th eira#i!ity to drink .e%era! cup. of coffee ine%ening and yet .!eep !ike a !ong on th eother hand there are peop!e who are .o.en.iti%e to caffeine that e%en a .ing!e cu pof coffee wi!! cau.e a re.pon.e #oarding onthe to<ic'

he <anthene #e%erage. a!.o create amedica! pro#!em' hey are dietary of a.timu!ant of the ,"&' 6ften the p h y . ician .face the =ue.tion whether to deny caff ein e-containing #e%erage. to patient. or not' I nfact chi!dren are more .u.cepti#!e th anadu!t. to e<citation #y <an th en e. '

0or thi. rea.on tea and coffee .hou!d #ee<c!uded from their diet' 7%en cocoa i. of







;

dou#tfu! %a!ue' It ha. a high tannin con ten tmay #e a. high a. 9> mg per cu p '

After a!! our main .tre.. i. on the p r e. en ceof caffeine in <anthene #e%erage. and .o inthi. project we wi!! .tudy and o#.er%e th e=u an tit of %ar y in i d iff er en. a

m p !e of tea







?

h eo r y

he mo.t important methy!ated a !k a!o idthat occur. natura!!y i. caffeine' I t .mo!ecu!ar formu!a i. ,

?H

1> "

56

/' It. I 8 PA,

name i. 1 4 ;-trimethy!<anthene an dcommon name i. 1-m e th y!a ted

th io # r om in e '






@

Pure!y it i. white cry.ta!!ine .o!id in th eform of need!e.' It. me!ting point i. 1/4 >c' I t i.the main acti%e princip!e component of tea!ea%e.' It i. pre.ent in tea !ea%e. up to4 and can #e e<tracted #y fir.t #oi!ing th etea !ea%e. with water which di..o!%e. m an yg!yco.ide compound. in addition to

caffeine' he c!ear .o!ution i. then tr ea tedwith !ead acetate to precipitate th eg!yco.ide compound. in the form of !eadcomp!e<' he c!ear fi!trate i. then e<tr actedwith e<tract. caffeine #ecau.e it i. m or e.o!u#!e in it then water'







1>

8.e. o f , aff e i n e :

1' In medicine it i. u.ed to . tim u !a tecentra! ner%ou. .y.tem and to in cr ea . ef!ow of u r in e '

/' Becau.e of it. .timu!ating eff ect .caffeine ha. #een u.ed to re!ie%e f a tigu e '

But it i. dangerou. and one m ayco!!ap.e if not con.ume. it under cert a in!im it '

4' ,affeine i. a!.o u.ed in an a!ge . icta#!et. a. it i. #e!ie%ed to #e a p a inre!ie%er' It i. a!.o #eneficia! inmigraine.'







11

7 ff ec t . o f , a f fe i n e

1' It i. p.ycho - . tim u !an t '

/' It impro%e. phy.ica! and menta! a # i!ity '

4' It. effect in !earning i. dou#tfu! #u tinte!!ectua! performance may im p r o%ewhere it ha. #een u.ed to reduce f a tigu e or

#or ed om '

5' hen admini.tered interna!!y it.timu!ate. heart and ner%ou. . y . te

mand a!.o act. a. diuretic' 6n th econtrary their e<ce..i%e u.e i. h ar m f u ! todige.tion and their !ong u.e !ead. tomenta! r etar d a tion '







1/

P r o ce du r e

0ir.t of a!! 9> gram. of tea !ea %e .were taken a. .amp!e and 19> m! of water wa. added to it in a #eak er '

hen the #eaker wa. heated up toe<treme #o i!in g 'he .o!ution wa. fi!tered and !ea dacetate wa. added to the f i!tr a ter !eading to the formation of a cu r d y

#rown co!oured p r eci p ita te '

e kept on adding !ead acetate ti!! n omore precipitate ha. #een f or m ed '

Again .o!ution wa. f i!ter ed ' "ow the fi!trate .o o#tained w a .heated unti! it had #ecome 9> m !'hen the .o!ution !eft wa. a!!owed tocoo !'After that /> m!' of ch!oroform w a .added to it '&oon after two !ayer. appeared in th e.eparating f u nn e!'






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e .eparated the !ower !ayer 'he .o!ution then e<po.ed to

atmo.phere in order to a!!owch!oroform to get e%a p or a ted '

he re.idue !eft #ehind wa. caff ein e 'hen we weighed it and recorded th eo # . er %ation . '

&imi!ar procedure wa. performed w ithdifferent .amp!e. of tea!ea%e. and =u an tity ofcaffeine wa. o#.er%ed in th em






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1.Red Label Tea (Brooke Bond)

Beight of china d i . h 5 ' > gm .Beight of china d i. hwith p r eci p it a te

5;'/> gm . '

Amount of caffeine >' > gm .

2.Yellow Label Tea (Lipton)

Beight of china d i . h 5 ' > gm .Beight of china d i. hwith p r eci p it a te

5;'19 gm . '

Amount of caffeine >'99 gm .






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3.Green Label Tea (Lipton)

Beight of china d i. h 5 ' > gm . '

Beight of chinad i. h with p r eci p it a te

5;'>9 gm . '

Amount of caffeine >'59 gm . '

1' Cuantity of caffeinein ed !a#e! teai. :>mg' D.am p!e of 9>gm'

/' Cuantity of caffeinein ye!!ow!a #e!tea i. 99mg'D.am p!e of 9> gm'

4' Cuantity of caffeinein green !a#e!teai. 59mg'D.am p!e of 9>gm'






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Eraphica!!y p!otting %ariou. tea .amp!e. inaccordance with the amount of caff ein e

pre.ent in them we pre.ent a .tunning f in d :

60mg

55mg

5mg

0.6

0.5

0.

0.#

0.$

0.%

0






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Order of quantities of affeine in

different sa!ples of tealea"es




(95368302) cbse xii chemistry project determination of caffeine in tea samples

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