8703302

PATENT ABSTRACTS

D N A C H A I N C O N T A I N I N G S T R E P T O M Y C I N - R E S I S T A N T

G E N E A N D C A P A B L E O F R E G U L A T I N G M A N I F E S T A T I O N

O F S A I D G E N E

Hamao UMEZAWA, Yoshiro OKAMI, 23, Toyotama Kita 4-chome, Nerima-ku, Tokyo 176. Japan assigned to ZAIDAN HOJIN B1- SEIBUTSU KAGAKU KENKYU KAI

A DNA fragment of about 3.8 kb which is pre- pared by cutting hybrid plasmid pST 141 of ab- out 12.6 kb. contained in Streptomyces griseus 4- I strain (FERM BP-1198) with a restriction enzyme Bglll and again cutting the resulting Bglll.BgllI-cut DNA fragment of about 7.0 kb with a restriction enzyme Sphl, said DNA frag- ment having restriction enzyme sites shown in the restriction enzyme map of Fig. 1. This DNA fragment contains a streptomycin-resistant gene and a portion capable of regulating manifesta- tion of the gene near the gene. When in- corporated in a proper actinomycetes plasmid vector, the fragment can form a hybrid plasmid surely functioning as a streptomycin-resistant selected marker.

8703303

A G R I C U L T U R A L - C H E M I C A L - P R O D U C I N G E N D O S Y M B I O T I C

M I C R O O R G A N I S M S A N D M E T H O D O F P R E P A R I N G A N D

U S I N G S A M E

Peter S CARLSON, Peter S CARLSON as- signed to CROP GENETICS INTERNA- TIONAL N V;

Hybrid agricultural-chemical producing micro- organism capable of entering into endo- symbiotic relationship with plants. The microorganisms are prepared by combining genetic material from an agricultural-chemical- producing microorganism and a plant infecting microorganism to form hybrid microorganisms and selecting from the hybrid microorganisms those hybrid microorganisms which are capable of producing an agricultural chemical, which do not create manifestations of disease in the plant host. and which are capable of entering into an endosymbiotic relationship with the plant host. Methods of use are also disclosed.

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8703304

I S O L A T I O N O F D N A S E Q U E N C E S E N C O D I N G L U C I F E R A S E

A C T I V I T Y A N D A P P L I C A T I O N S O F T H E S A M E

Marlene DeLuca McELROY. Donald Raymond HELINSKI. Keith Vernon WOOD, WET Jeffrey Roux DE assigned to THE RE- GENTS OF THE UNIVERSITY OF CALIFORNIA

DNA compositions and methods of con- structing and using the same consisting of DNA sequences encoding luciferase activity, or DNA sequences encoding hybrid molecules exhibiting luciferase activity and a second biological ac- tivity that are useful in performing biological assays.

8703451

A N T I - S E N S E R N A F O R T R E A T M E N T O F R E T R O V I R A L

D I S E A S E S T A T E S

Paul E NEIMAN, Richard Yue-Leung TO, Shane C BOOTH assigned to FRED HUT- CHINSON CANCER RESEARCH CENTER

A method for conferring resistance to infection by pathogenic retroviruses. Cells susceptible to infection by a pathogenic retrovirus are trans- formed with a polynucleotide directing trans- cription of RNA that is complementary to a region of the retroviral genome and that is ef- ficace to interrupt retroviral replication by sub- stantially preventing formation of pathogenic virions in the treated cell and its progeny. The cells are preferably explanted during the therapeutic transformation step, then in- troduced into the treated host.

8703619

P R O C E S S F O R E N H A N C I N G T R A N S L A T I O N A L E F F I C I E N C Y

O F E U K A R Y O T I C M R N A

Lee GEHRKE assigned to MAS- SACHUSETTS INSTITUTE OF TECH- NOLOGY

A process and means for increasing the produc- tion of protein translated from eukaryotic mes-