6- Microbial Nutrition & Growth

Bacterial growth

electron source lithotrophs (inorganic)

organotrophs (organic)

CHNOPS

Singlet oxygen

Toxic forms of oxygen

contains O2 2- (peroxide anion)

Detoxification enzymes

Other antioxidants: carotenoids, glutathione, Vit E & C, etc.

Micro-aerophiles

Nitrogen cycle

Fig 26.17

acidophiles

basophiles/ alkalinophiles

Vibrio

Ferroplasma

neutrophiles

Fig. 2.14

Halophiles

obligate

tolerant

Barophiles

Extremophiles

Ecological associations

Antagonistic

Synergistic

Symbiotic

Biofilms

Quorum sensing

Other kinds of samples:

food, water, air, soil, etc.

Streak plate

Pour plate

Spread plate

Inoculating needle & loop

pipet & pipetters

micropipette = micropipet = micropipetter

Disposable tip

tubes

Petri plate

flask

containers

For beer

agar broth agar

slant deep

agar

(for Nutrient Broth: just leave out the agar)

General medium Selective medium

(nutrient agar) (MacConkey agar)

Differential medium

(MacConkey agar)

Differential media (newer)

candle jar

capnophilics

Anaerobic jar Glove box

Laminar flow hood

(for aerobes)

Standard freezer -20oC

Ultra deep freezer -70 to -86oC

Liquid nitrogen -196oC

refrigerator

Lyophilization = freeze-drying

Other: dessication, crystallization

binary fission

budding

fragmentation

Growth curve

Plate count using serial dilutions on spread plates

Colony counter

Cell counter

MPN

26 cells /100 ml

(from T6.6)

Most Probable Number

dry weight

Indirect methods

metabolic activity

Turbidity

spectrophotometer