5th european iabpa conference rome, 12-15 may 2015 reflectance spectroscopy on blood stains absorbed...

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5th European IABPA Conference Rome, 12-15 May 2015 Reflectance spectroscopy on blood stains absorbed by different backgrounds. Davide Manzini Madatec Srl (MADATEC.COM) Pessano con Bornago (MI), Italy

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5th European IABPA Conference Rome, May 2015 Ex vivo HEMOGLOBIN 3 Hb HbO 2 Met-Hb Hemichrome oxygenation oxydation

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Page 1: 5th European IABPA Conference Rome, 12-15 May 2015 Reflectance spectroscopy on blood stains absorbed by different backgrounds. Davide Manzini Madatec Srl

5th European IABPA ConferenceRome, 12-15 May 2015

Reflectance spectroscopy on blood stains absorbed by

different backgrounds.

Davide Manzini Madatec Srl (MADATEC.COM)Pessano con Bornago (MI), Italy

Page 2: 5th European IABPA Conference Rome, 12-15 May 2015 Reflectance spectroscopy on blood stains absorbed by different backgrounds. Davide Manzini Madatec Srl

Gerda Edelman ([email protected])Maurice Aalders, Ton van Leeuwen

Academic Medical Center, Biomedical Engineering and PhysicsAmsterdam, The Netherlands

Vicky Manti, Saskia van RuthWageningen UR, RIKILT Institute of Food Safety

Wageningen, The Netherlands

5th European IABPA ConferenceRome, 12-15 May 2015

Thanks for the work done by:

Page 3: 5th European IABPA Conference Rome, 12-15 May 2015 Reflectance spectroscopy on blood stains absorbed by different backgrounds. Davide Manzini Madatec Srl

5th European IABPA ConferenceRome, 12-15 May 2015

500 600 700 800

10

20

30

Wavelength (nm)

Abs

orpt

ion

(mm

-1)

HbO2MetHbHC

Ex vivo

HEMOGLOBIN

3

500 550 600 650 700 750 800-2

-1.5

-1

-0.5

0

0.5

1

1.5

Wavelength (nm)

Cor

r. re

flect

ance

(-)

1 min1 hour1 day1 week

Hb HbO2

Met-Hb

Hemichrome

oxygenation

oxydation

Page 4: 5th European IABPA Conference Rome, 12-15 May 2015 Reflectance spectroscopy on blood stains absorbed by different backgrounds. Davide Manzini Madatec Srl

5th European IABPA ConferenceRome, 12-15 May 2015

Thanks for the work done by:M.llo Capo Rosario Casamassima,

Forensic Science Department of the Arma dei Carabinieri (Rome)

Page 5: 5th European IABPA Conference Rome, 12-15 May 2015 Reflectance spectroscopy on blood stains absorbed by different backgrounds. Davide Manzini Madatec Srl

5th European IABPA ConferenceRome, 12-15 May 2015

The elements needed to build

a Reflectance Spectroscopy system (VIS):

Page 6: 5th European IABPA Conference Rome, 12-15 May 2015 Reflectance spectroscopy on blood stains absorbed by different backgrounds. Davide Manzini Madatec Srl

Spectra taken with the previous

system on sample E (blood on white paper)

Page 7: 5th European IABPA Conference Rome, 12-15 May 2015 Reflectance spectroscopy on blood stains absorbed by different backgrounds. Davide Manzini Madatec Srl

Same spectra taken on sampleE in derivative mode

(differential, 20 points)

5th European IABPA ConferenceRome, 12-15 May 2015

Page 8: 5th European IABPA Conference Rome, 12-15 May 2015 Reflectance spectroscopy on blood stains absorbed by different backgrounds. Davide Manzini Madatec Srl

Spectra taken on sample A(dark carpet)

5th European IABPA ConferenceRome, 12-15 May 2015

Page 9: 5th European IABPA Conference Rome, 12-15 May 2015 Reflectance spectroscopy on blood stains absorbed by different backgrounds. Davide Manzini Madatec Srl

Thanks for your attention ;-)

5th European IABPA ConferenceRome, 12-15 May 2015

Fonte dell'immagine: M. C. Misiti / Istituto Centrale per il Restauro e la Conservazione del Patrimonio Archivistico e Librario, (ICRCPAL) Roma