114 PATENT ABSTRACTS

genetic analyses are disclosed herein. Also dis- closed is a method for obtaining Variable Tan- dem Repeat polymorphism at a single genetic locus as well as other genetic analyses.

5192659

I N T R O N S E Q U E N C E A N A L Y S I S M E T H O D F O R D E T E C T I O N O F

A D J A C E N T A N D R E M O T E L O C U S A L L E L E S A S

H A P L O T Y P E S

Malcolm J Simons, Fryerstown, Australia as- signed to GeneType AG

The present invention provides a method for detection of at least one allele of a genetic locus and can be used to provide direct determination of the haplotype. The method comprises am- plifying genomic DNA with a primer pair that spans an intron sequence and defines a DNA sequence in genetic linkage with an allele to be detected. The primer-defined DNA sequence contains a sufficient number of intron sequence nucleotides to characterize the allele. Genomic DNA is amplified to produce an amplified DNA sequence characteristic of the allele. The am- plified DNA sequence is analyzed to detect the presence of a genetic variation in the amplified DNA sequence such as a change in the length of the sequence, gain or loss of a restriction site or substitution of a nucleotide. The variation is characteristic of the allele to be detected and can be used to detect remote alleles. Kits comprising one or more of the reagents used in the method are also described.

5192669

M E T H O D T O P R O D U C E R E C O M B I N A N T P R O T E I N S

U S I N G A N E X P R E S S I O N V E C T O R C O M P R I S I N G

T R A N S C R I P T I O N A L A N D T R A N S L A T I O N A L A C T I V A T I N G

S E Q U E N C E S

Brigitte E Schoner, Ronald Schoner, Fryers- town assigned to Eli Lilly and Company

The present invention is composed of novel recombinant DNA expression vectors which contain a transcriptional activating sequence, a translational activating sequence and a DNA sequence coding for a functional polypeptide,

especially bovine growth hormone. The af- orementioned translational activating sequences contain a ribosome binding site and are designed to provide high level expression of DNA that codes for virtually any functional polypeptide. The invention further provides transformed microbial host cells capable of producing bovine growth hormone and other functional poly- peptides at high levels.

5 1 ~ 6 ~

M U T A N T S T R A I N O F C. A C E T O B U T Y L I C U M A N D P R O C E S S F O R M A K I N G

B U T A N O L

Mahendra Jain, Daniel Beacom, Rathin Datta assigned to Michigan Biotechnology Institute

A biologically pure asporogenic mutant of Clostridium acetobutylicum is produced by growing sporogenic C. acetobutylicum ATCC 4259 and treating the parent strain with ethane methane sulfonate. The mutant which as been designated C. acetobutylicum ATCC 55025 is useful in an improved ABE fermentation pro- cess, and produces high concentrations of butanol and total solvents.

5192675

C L O N E D K P N I R E S T R I C T I O N M O D I F I C A T I O N S Y S T E M

Deb K Chatterjee, Alan Hammond assigned to Life Technologies Inc

The present invention discloses the cloning and expression in a host such as Escberichia coli of the KpnI restriction-modification system from Klebsiella pneumoniae, utilizing a two step pro- tocol. Initial protection of the E. coli host with methylase expressed on a vector was required to stabilize a compatible vector carrying both the endonuclease and the methylase genes on a single DNA fragment. A chromosomal map was generated localizing the genes for Kpnl methylase and endonuclease. An E. coli strain was constructed which produced several thousand-fold higher levels of KpnI endo- nuclease than the level produced by Klebsiella pneumoniae. This invention is also directed to cloning and expression of genes encoding for restriction endonuclease isosehizomers of KpnI and/or modification methylase isosehizomers of KpnI methylase.