634 PATENT ABSTRACTS

4894338

Y E A S T S T R A I N S P R O D U C I N G C E L L U L O L Y T I C E N Z Y M E S A N D

M E T H O D S A N D M E A N S F O R C O N S T R U C T I N G T H E M

Jonathan Knowles, PenttilaMerja, Tuula Teeri, Helena Nevalainen, Irma Salovuori, Paiv Lehtovaara-Helenius, Helsinki, Finland as- signed to Oy Alko AB

The full length DNA sequence encoding the ma- ture cellobiohydrolase II(CHBII), as well as the DNA sequence encoding the CBHII signal sequence, have been isolated and cloned from the fungus Trichoderma reesei. Recombinant vectors comprising these sequences, and yeast hosts transformed with such vectors are dis- closed. Also disclosed are methods for con- structing the vectors and hosts according to the invention, as well as methods for expressing the disclosed sequences. CBHII is involved in hydro- lysis of cellulose.

4895806

D E V I C E F O R L I Q U I D C H R O M A T O G R A P H Y O R I M M O B I L I Z E D E N Z Y M E

R E A C T I O N

S Le Minh, James A Sanderson, Gateshead, United Kingdom assigned to Millipore Ireland B V

A device for separating molecular components from liquids. A multi-layer microporous mem- brane assembly is located within the housing (1) with edges of each membrane within the as- sembly lying adjacent to a barrier. Means (5,6) are provided for substantially preventing flow between the membrane edges and the barrier, so that flow is through the membranes in series. In- let means (2) direct incoming liquid on to a first surface of the membrane assembly, and outlet means (3) gather and direct outgoing liquid from a second surface of the membrane assembly. The membrane material of the assembly has a ligand or an immobilized enzyme bound thereto for ef- fecting chromatographic separation or an im- mobilized enzyme reaction.

4894339

I M M O B I L I Z E D E N Z Y M E M E M B R A N E F O R A

S E M I C O N D U C T O R S E N S O R

Yoshio Hanazato, Satoru Shiono, Mamiko Nakako, Satoshi Yamada, Hon, Japan assigned to Seitaikinouriyou Kagakuhin Sinseizogijutsu Kenkyu Kumiai

A membrane containing an immobilized enzyme for a semiconductor sensor is prepared con- taining a water soluble photosensitive resin in- cluding a high molecular weight polyvinyl pyrrolidone crosslinked to 2, 5-bis (4'-azide-2'- sulfobenzal) cyclopentanone sodium salt, and an enzyme. Glutaraldehyde and bovine serum albumin, polyamino acid or polyamino amino acid copolymer may also be present to provide chemical crosslinking. The enzyme may be glucose oxidase, urease or lipase. The membrane can be directly formed on ion-sensitive protions of a pH-ion sensitive field effect transistor to form a semiconductor sensor by coating an aqueous solution of the resin and enzyme on the ion-sensitive portion, drying and irradiating with light such as ultraviolet light to provide photo crosslinking.

4897346

S T A B I L I Z E D L I Q U I D E N Z Y M E C O M P O S I T I O N F O R G L U C O S E

D E T E R M I N A T I O N

Thomas H Gawronski assigned to Beckman In- struments Inc

A homogeneous liquid enzyme reagent for the quantitative determination of glucose comprises the enzymes hexokinase and glucose-6- phosphate dehydrogenase in a solution of glycerol (30% v/v) in water, and a stablizer sys- tem comprising a heavy metal ion chelating a- gent. Preferably the stabilizers also comprise an antioxidant and a microbic-control agent. The stabilizers are in sufficient amounts such that the enzyme reagent has a shelf life of at least about two years when stored at a temperature of from 2 degrees to 8 degrees C.

4897352

A C R Y L A T E B A S E D A D S O R B E N T R E S I N F O R T H E

I M M O B I L I Z A T I O N O F E N Z Y M E S

Yohannes Chonde, Marsha A Paul assigned to The Dow Chemical Company