462 PATENT ABSTRACTS

4863851 cancerous state. Compositions and methods based on the disclosed peptides for detection and

M O N O C L O N A L A N T I B O D Y T O treatment of cancer and other proliferative dis- P R O S T A T E S E C R E T O R Y eases and for cell or tissue growth associated

P R O T E I N treatment, e.g., wound healing, ulcer therapy and bone loss are also described.

Robert McEwan, Donald Carter assigned to The Upjohn Company 4863955

Provided are hybridomas for producing mono- clonal antibodies against human prostate S C Y T O P H Y C I N S secretory protein (PSP15), anti-PSP15 mono- clonal antibodies, fragments and derivatives Richard Moore, Eiich Furusawa, Ted R Norton, thereof, and methods for their use. The anti- Gregory M L Patterson, Jon S Mynderse as- bodies are monospecific, bind to protein A and signed to Eli Lilly and Company; University of are of the IgM class of immunoglobulins. The Hawa monoclonal antibodies are useful as basic dia- gnostics for detecting human prostate cancer Scytophycins A, B, C, D and E, an aldehyde and cells, alcohol derivative of scytophycin B and the

specified alkanoyl and benzoyl esters of 4863854 scytophycins A, B, C, D and E and the B

derivatives (seytophycin compounds) are novel antineoplastic and antifungal agents. Antifungal

M O N O C L O N A L A N T I B O D I E S T O compositions containing a scytophycin com- M U C I N - L I K E H U M A N pound and methods of inhibiting fungi, espe-

D I F F E R E N T I A T I O N A N T I G E N S cially plant pathogenic fungi, using these compositions are included. A method for pro-

M Jules Mattes, John Lewis, Lloyd Old, Ken- ducing the scytophycin complex, comprising neth O Floyd, Katherin Look assigned to Sloan- scytophycins A, B, C, D and E, using a new Kettering Institute for Cancer Research strain of the blue-green alga Scytonema pseudo-

hofmanni, and a biologically purified culture of Three new monoclonal antibodies, MU78, the alga are also provided. MT334, and MQ49, and the hybridoma cell lines producing these, are disclosed. The antibodies specifically bind to mucin-like antigens with dist- 4864019 ribution over various carcinomas.

A N T I B O D I E S T O I N H I B I N AND CONJUGATES PRODUCED

4863899 T H E R E F R O M

B I O L O G I C A L L Y A C T I V E Wylie W Vale, Jean E F Rivier, Joachi Spiess as- P O L Y P E P T I D E S signed to The Salk Institute for Biological

Studies George J Todaro

A 32,000-Dalton protein with inhibin activity is Novel biologically active polypeptides, in- isolated from porcine follicular fluid which is cluding a new class of transforming growth fac- composed of two chains having molecular tor (TGF) polypeptides, which exhibit cell weights of about 18,000 and about 14,000 Dal- growth promoting properties are disclosed, as tons, which are bound together by disulfide bon- well as a process for isolating the TGF poly- ding. Microsequencing revealed the NH2- peptides from both human and murine cell lines terminal portion of the 18 kD chain to be Ser- in hom6geneous form. Also disclosed are anti- Thr Ala-Pro-Leu-Pro Trp-Pro-Trp-Ser-Pro-Ala genic oligopeptides derived from the TGF Ala-Leu-Arg-Leu-Leu-GIn-Ar g-Pro-Pro-Glu- polypeptides and antibodies raised therefrom Glu-Pro-Ala-Val and of the 14 kD chain to be which have application in the detection and Gly-Leu-Glu-Cys with the next 21 residues treatment of malignancies and oligipeptides believed to be Asp-Gly-Ser-His-Asn-Leu-Asp which have the ability to bind with cellular Ser-Arg-GIn-Gln-Phe-Phe Ile-Asp Phe-Arg- growth factor receptors and thus to interfere Leu-Il e-G y-Trp. This 32 kD protein specifically with transformation of certair k cell lines into a inhibits basal secretion of FSH, but not of LH.

PATENT ABSTRACTS 463

Antibodies raised against a synthetic replicate of 4865841 the N-terminal six residues of the 18 kD chain are effective to reduce the activity of highly M E T H O D S A N D C O M P O S I T I O N S purified 32 kD inhibin and may be administered FOR TRANSIENT ELIMINATION to mammalian animals for the purpose of O F H U M O R A L I M M U N E neutralizing endogenous inhibin and thereby in- A N T I B O D I E S creasing gonadotropin secretion. The antibodies are also useful in immunoassays to determine in- hibin levels in mammalian fluids. To generate Joseph P Baiint, Sharo Cochran, Frank Jones, the antibodies, short chain peptides are syn- Harry Snyder assigned to lmre Corporation thesized which mimic portions of natural mam- malian inhibin protein chains, the synthetic Antibody is removed from the blood of patients peptides are conjugated to large carrier who are being treated or diagnosed with ex- molecules and the conjugates are used as in- ogenous macromolecules, such as immuno- oculum to induce a mammalian immune system toxins. As the immunotoxins are foreign to produce inhibin-reactive antibodies, antigens, the patient will" develop an immune

response. The antibodies produced by the im- mune response upon subsequent immunization

4864020 with the immunotoxins will interfere with the desired function of the immunotoxins by pre-

M O N O C L O N A L A N T I B O D I E S venting them from binding with their specific A N D C E L L L I N E S F O R target site. By extracorporeally removing the

P A R A T H Y R O I D T I S S U E - S P E C I F I C antibodies, the activity of the immunotoxins can A N T I G E N P A R A T H Y R O I D - be enhanced.

S P E C I F I C

4865971 William G Cance, Joseph M Davie, Samuel A Wells, William G Dilley, Michael J Welch, M O N O C L O N A L A N T I B O D Y Jeremiah J Morrissey assigned to Washington S P E C I F I C T O A C O M M O N University School of Medicine D E T E R M I N A N T S I T E O F

Monoclonal antibodies specific for an antigen N E U T R O P H I L S A N D present on the surface of parathyroid tissue are E O S I N O P H I L S useful in imaging such tissue when conjugated to suitable label. The antibodies of the invention Kenneth Kortright, David E Hofheinz, Gary bind exclusively to parathyroid surfaces and do Toedter assigned to Coulter Corporation not bind to other tissues. The antibodies are use- ful in establishing the location of the parathyroid A hybrid cell line capable of producing mono- whether in its normal location or in ectopic clonal antibodies uniquely specific to human placements. An exemplary monoclonal has been neutrophils and eosinophils and exhibiting no deposited at the American Type Culture Collec- specificity for lymphocytes, basophils and tion and has accession number ATCC No. monocytes. Further thereisnoreactivitywithac- HB9917. ute leukemia cells. One of the partners in the

hybridoma fusion of a mouse spleen cell 4865835 developed from using human granulocytes as the

immunization agent. The monoclonal antibody further is capable of being used to enumerate and

D I A G N O S I S A N D T R E A T M E N T isolate neutrophils in normal peripheral blood OF TUMORS and possibly in blood of patients with acute

leukemia. Richard H J Begent, London S W 19, United Kingdom 4865980

There are disclosed liposomes to which are M O N O C L O N A L A N T I B O D I E S linked agents which are capable of binding an anti-tumor antibody. The liposomes are useful in F O R D N A - R N A H Y B R I D the detection and treatment of tumors by reason C O M P L E X E S A N D T H E I R U S E S of their ability to remove non-specifically dist- ributed anti-tumor antibodies from vascular and W Dorse Stuart, Mark B Frank assigned to other extra-cellular spaces. University of Hawaii