788 PATENT ABSTRACTS

from human sources from blood and tissue of other animals. The method and products are particularly useful in forensic investigations. First, mice are injected with purified human serum albumin, spleen cells from the mice are fused with cells from a murine myeloma. Resul- ting hyberdomas are screened on human serum albumin coated plates. Subcloning, stabilizing by culture and continued assays for anti-human albumin activity produces products which recognize and differentiate blood or tissue sam- ples from samples of other species.

provide a plurality of liquid dilution and reagent materials to a serum sample, the test module having programmable valve means coacting with a dispense probe to serially: select a serum sample and to deposit said sample and liquid dilution material into a dilution cup or vessel; subsequentially withdraw a portion of the diluted serum from the dilution cup and to dis- pense said diluted serum portion along with at least one liquid reagent material into a reaction cup or vessel.

4735899

D E T E C T I O N O F A I R B O R N E M I C R O O R G A N I S M S

David Stuart, John M Eagleson assigned to The Baker Company lnc

Disclosed is a process and test kit for deter- mining the reliability of a barrier to micro- organism passage. In accordance with the process, a multiplicity of non-self replicating particles is introduced into a first gas volume, disposed on one side of a barrier to particle movement, and particle traps are placed in a second gas volume on the other side of the bar- rier. The particles carry a marker substance such as a toxin for a selected microorganism or a com- ponent needed for growth of a selected microorganism. The barrier is operated and the contents of the trap are assayed for the amount of the marker substance present in association with particles which crossed the barrier by adding the marker substance in the trap to a cul- ture of the selected microorganism and detecting the effect of the marker substance on the culture. Finally, the results of the assay are compared to a standard to determine the number of trapped particles which crossed the barrier. The process is capable of detecting the presence of less than 10 and as few as a single one of the particles.

4737342

4737455

A N A L Y T I C A L U T I L I Z A T I O N O F P H A G O C Y T E C E L L L I N E S

Patrick De Baetselier, Berchem, Belgium as- signed to 501 N V Innogenetics

The property of phagocyte cells to show chem- iluminescence when activated by certain chem- ical or immunological agents is used for a qualitative and quantitative analysis of such a- gents in biological fluids. A variety of factors such as opsonizing anti-bodies, complement, in- hibitors, membrane-specific anti-bodies, and their antigens, membrane-specific lectins, lymphokines, endotoxins, toxic substances and others can be analyzed in this way. The new method finds application in the biotechnical, clinical, immunological and pharmacological fields, with a special preference for toxicological screening of pharmaceuticals. The phagocyte cells needed for the analysis can be derived from a continuous phagocyte cell line and a method of producing such a cell line is provided.

4737458

A M I N O P Y R A Z O L I N O N E S , R E A G E N T C O N T A I N I N G T H E M

A N D T H E U S E T H E R E O F I N T H E E N Z Y M A T I C D E T E R M I N A T O N

O F H Y D R O G E N P E R O X I D E

T E S T M O D U L E

Raymond J Herrmann, Harry F DeBevec, Ran- dolph Bradshaw, Peter O Botten assigned to Ciba Corning Diagnostics Corp

A test module or reagent handling device for use with a clinical analyzer for conducting body serum assays and/or immunoassays; the test module coacting with the analyzer to selectively

Hans-Georg Batz, Rupert Herrmann, Joachim Siedel, Tutzing, Federal Republic Of Germany assigned to Boehringer Mannheim GmbH

The present invention provides amino- pyrazolinones of the general formula: See Patent for Chemical Structure wherein R l, R2 and R3, which can be the same or different, are straight- chained or branched alkyl radicals containing up to 6 carbon atoms and together containing at