30 jun microsopy

8/21/2019 30 Jun Microsopy

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Microscopy

What is a microscope?


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Microscope

Microscope is a toolwhich can help you seetiny objects and livingorganisms. It makesthem look bigger.

This ability of the

microscope is called itsmagnifying power ormagnication.


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Light microscope

Light microscope uses diused light from thesun or articial light to illuminate the object tobe observed.


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Microscopy

What are the parts of the microscope?


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1.Arm

upports the body tube.


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2. Base

upports and stabili!es themicroscope.


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3. Eyepiece / ocular lens

Magnies image producedby objective lens.


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4. Body Tube

Maintains the properdistance between the

objective and ocular

lenses.


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Moves stage up and downa large amount for

"#$%I&'.

5. Coarse adjustment knob


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Moves stage up and downa tiny amount to

()*+,& the image.

6. Fine adjustmentknob


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7. Light source

-lamp or mirror +rovideslight for viewing the slide.

+rojects light %+/)*0

through the diaphragm1

the +,$IM,&1 and

the L,&,.


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8. Diaphragm

$ontrols the amount oflight passing through

the slide.


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tage clips2 hold the slide in place.

tage

2 upports the slide

being viewed.

9. Stage and stage clips


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10. Objective lenses

"ocus and magnifylight coming through the

slide.


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*otates to allow use of dierent power objectives.

11. Revolving nosepiece


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upports the arm andcontrols the body of the

microscope.

12. Inclination joint


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Follow these instructions when using the

microscope.

1. To carry the microscope, grasp the

ARM with one hand. Hold the BASE withyour other hand.

Using the Microscope in 10 Steps


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34 teps to

%sing the Microscope. Ad!ust the "#A$HRA%M while loo&ing

through the E'E$#E(E.

). Ad!ust the *#%HT S+R(E -mirror until

you see the circle o/ light.


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34 teps to%sing the Microscope

0. $lace the slide on the STA%E.

. (enter the S$E(#ME2 o3er the small opening on the STA%E.

4. Secure the slide with the STA%E (*#$S.


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5. 6hile loo&ing at the STA%E /rom the side,

turn the (+RSE F+(S 72+B to lower

the B+"' TBE until the *+6 $+6ER +B8E(T#9E almost touches the slide.


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:. 6hile loo&ing through the E'E$#E(E, care/ully

mo3e the (+RSE F+(S 72+B until the

specimen comes into 3iew.

;. To switch to the H#%H $+6ER +B8E(T#9E

*E2S, loo& at the microscope /rom the side.


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1 into place. #/ the

lens hits the slide, the slide and?or lens might

@rea&.

Turn the F#2E F+(S 72+B until the

specimen comes into /ocus.


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Specimen


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Answer Each Question

3. The lens closest to your eye when you use amicroscope is the 5555555555.

6. /hen using the microscope1 always beginwork with the 5555555555 objective rst.

7. /hich knob should you never use while onmedium or high power8 ,9plain why.

:. The lens closest to the slide is the 55555555555.

;. /hen using the coarse focus knob1 how

should you be view the slide8 ,9plain why.


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,nd of discussion

30 jun microsopy

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