A Complete System ApproachCombine the D3 DFA HSV Identification or Identification and Typing Kit with

H&V-Mix™* MixedCells™, Flocked Swabs and UTM for a complete system

approach to your Herpes Simplex Virus testing needs.

Rapid Turnaround TimeMost lesion or genital herpes cultures show positive results within 24 hours on

H&V-Mix MixedCells. Confirm CPE in as little as one 15 minute staining step.

Flexible Reagent FormatsOrder as a complete kit containing reagents, control slides, PBS and mounting

fluid, or order reagents independently as an Identification and Typing Duo Pack

(HSV-1 and HSV-2 Reagents packaged together) or Single HSV Identification

Reagent. It’s your choice!

Herpes Simplex Virus

identification within

24 hours using proven

Totally Tubeless™ MixedCells

technology from Diagnostic

HYBRIDS.

Herpes Simplex VirusIdentification & Typing Kits

D3® DFA

H&V-Mix™ cells infected with Herpes Simplex Virus Type 1, stained with D3 DFA Herpes Simplex Virus Identification Kit

H&V-Mix™ cells infected with Herpes Simplex Virus Type 2, stained with D3 DFA Herpes Simplex Virus Identification Kit

*US Patent No. 6,168,915 & 6,495,316

A Quidel Company For More Information, Contact Us www.dhiusa.com (866) 344-3477© 2010 Quidel Corporation. All rights reserved.

A Quidel Company For More Information, Contact Us www.dhiusa.com (866) 344-3477© 2010 Quidel Corporation. All rights reserved.

MKT0006v2010MAR26

H&V-Mix Product Codes

D3 DFA HSV KitsKits

01-080000 D3 DFA HSV Identification Kit

Includes: HSV Reagent (5-mL), 40X PBS Concentrate (25-

mL), Mounting Fluid (7-mL), HSV-1/HSV-2 Antigen Control

Slides (5 slides)

01-090000 D3 DFA HSV Identification & Typing Kit

Includes: HSV-1 Reagent (5-mL), HSV-2 Reagent (5-mL),

40X PBS Concentrate (25-mL), Mounting Fluid (7-mL), HSV-

1/HSV-2 Antigen Control Slides (5 slides)

Individual Components

01-090001 D3 DFA Herpes Simplex Virus

Identification & Typing Reagent

Duo Pack

Includes: HSV-1 DFA Reagent (5-mL), HSV-2 DFA

Reagent (5-mL)

01-085005 D3 DFA Herpes Simplex Virus

Reagent (5-mL)

01-00100 HSV-1/HSV-2 Antigen Control Slides

(10 slides per set)

01-090025 40X PBS Concentrate (25-mL)

01-002007b Mounting Fluid (7-mL)

Vials

98-0102 Shell Vials (with coverslip)

Plates

98-2412 24 well/12 fill

98-2418 24 well/18 fill

98-2424 24 well/24 fill

98-4812 48 well/12 fill

98-4818 48 well/18 fill

98-4824 48 well/24 fill

Standard 2% FBS Refeed Medium

10-320100 100-mL

10-320500 500-mL

Recommended Protocol

Clinical Specimens

HSV ID and Typing

Shell Vial 2Shell Vial 1

Inoculate 2 H&V monolayers with 200 to 400-µL of specimen

Stain with HSV-2 DFA Reagent for 15-30 min.

Stain with HSV DFA Reagent for 15-30 min

Examine for the presence of Fluorescent Stained Cells

Fluorescent Stained Cells present

Report “Herpes Simplex Virus Detected”

No Fluorescent Stained Cells present

Report “NO Herpes Simplex Virus Detected”

Stain with HSV-1 DFA Reagent for 15-30 min.

Centrifuge 700xg, 1-Hours, Incubate @ 35˚ - 37˚ Cfor 1 to 2 days

Examine for the presence of Fluorescent Stained Cells

Examine for the presence of Fluorescent Stained Cells

Fluorescent Stained Cells

present

Report “Herpes Simplex Virus

Type 1 Detected”

Report “NO Herpes Simplex Virus Detected”

Report “Herpes Simplex Virus

Type 2 Detected”

No Fluorescent Stained Cells

present

Fluorescent Stained Cells

present

HSV ID

Inoculate H&V monolayers with 200 to 400-µL of specimen

Centrifuge 700xg, 1-Hours, Incubate @ 35˚ - 37˚ Cfor 1 to 2 days

Hs27 ReadyCells® SystemHs27 ReadyCells

F-87-0102-24 24 Shell Vials - With Coverslip

ReadyCells Heat Block

99-610-009 ReadyCells Heat Block (120 volts)

(used for rapid, controlled thawing

of ReadyCells)

Herpes Simplex VirusIdentification & Typing Kits

D3® DFA