29 september and 1 october, 2004 chapter 11 transposition and site- specific recombination
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29 September and 1 October, 2004
Chapter 11
Transposition and Site-Specific Recombination
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Overview• Conservative Site-Specific Recombination (CSSR) may involve
insertion, deletion, or inversion of DNA sequences.• Site-specific recombinases have a mechanism that includes a DNA-
protein covalent intermediate.• CSSR may be regulated by the presence or absence of accessory
proteins.• Resolvases are CSSR recombinases that disentangle circular
chromosomes.• Transposons move using recombination pathways.• Transposons may be autonomous or nonautonomous.• DNA transposons and viral-like retrotransposons move via a cut/paste
mechanism.• Retrotransposon movement involves reverse transcription.• Some transposons regulate copy number or control target site selection
through the use of proteins or antisense RNA.• V(D)J recombination uses regulated, specific recombination to generate
immune diversity.
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Recombination and Transposition
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CSSR: Prophage Insertion
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Three Classes of CSSR
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Recombinase Recognition Sites
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Recombinase Mechanism
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Serine Recombinases
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Tyrosine Recombinases
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Mechanism of Cre
Recombinase
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Cre-DNA Structure
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Lambda integration
requires architectural
proteins.
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Int and IHF stabilize bent
DNA.
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Hin Inversion
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Hin inversion requires Fis bound at an enhancer.
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Resolvases disentangle circular DNAs after replication.
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FtsK Regulation of
the Xer Resolvase
Mechanism
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FtsK is present at the division closure site.
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Conservative and Replicative Transposition
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Transposons in Several Genomes
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Types of Transposons
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Cut and Paste Transposition
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Three Mechanisms for Cleaving the Nontransferred Strand
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Replicative Transposition
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Retrotransposon Movement
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Retrotransposon Movement
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Retrotransposon Movement
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DNA Transposases and retroviral integrases are members of the same protein superfamily.
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LINE poly-A Retrotransposon
Movement
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Tn10 achieves antisense copy control by overlapping promoters.
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Antisense Copy
Control
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Tn10 transposase promoter is active only
when hemimethylated.
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MuA and MuB participate in selecting Mu transposition
target sequences.
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MuA disrupts MuB assembly,
conferring transposition
target immunity.
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Clustered Integration of Yeast Ty Elements
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Antibody
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V(D)J Recombination
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Recombination Signal Sequences
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V(D)J Recombination
Mechanism
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Title
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LINES and SINES Again?