IONICS Mass Spectrometry- Boldy going….- Triple Quad Mass Spectrometry- A Big Year for IONICS

Erik HansenCPSA 2012

Global Headquarters

• Greater Toronto Area(Bolton)

• Est 2001• 40+ employees

• R&D, Manufacturing, Service, Marketing and Sales

• Global distribution / OEM Partnerships

Evolution at IONICS

IONICS - Prequel IONICS New GenerationTriple Quad – 3Q Series Amolecular Analyser

3Q Series A Molecular Analyzer

• Flow-Based Mass Spectrometry– Its like fuel injection vs

regular carburation– Launched in 2010– Innovations in

• HSID – Laminar Flow ION Guide- Collision Cell Unifield™ Detector (combination)

– Vertical Design

Evolution Continues at IONICS

IONICS - Upgrades IONICS New GenerationSeries A3Q Molecular Analyzer

IONICS Next Generation 3Q Series 100, 3Q Series 200

IONICS Latest Generation 3Q Series 300

3Q 3003Q 100 3Q 200

TerrestrialDeep SpaceNear Space

Mass Range 5-2150 m/z

Mass Range 5-1500 m/z

Mass Range 5-800 m/z

IONICS Family of Triples

MolAna Software

MolAna LC-MRM Quantitation Software for the High Performance 3Q Triple Quad Molecular Analyzers.

Software features• Easy to learn workflow • Modularized data acquisition, batch creation,

data viewing and quantitation• Import export capabilities for each module

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 10

50

100

150

200

Blank

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 10

50

100

150

200

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 10

20406080

100120140160180200

500ag/μLS/N=27

250ag/μLS/N=13

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 10

100200300400500600700800900

2.0fg/μLS/N=110

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 10

500

1000

1500

2000

2500

10.0fg/μLS/N=560

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 10

5000

10000

15000

20000

25000

100.0fg/μLS/N=5530

LC-MRM for Reserpine 609.2195.1 transition3Q 300 series

0.1 1 10 10010

100

1000

10000

100000

f(x) = 569.004006982465 x − 35.5403574545744R² = 0.999842999078157

Concentration

Area

(cou

nts)

Shimadzu Prominence UFLCImtakt Cadenza CD-C18HT (50x2.1 mm, 3µm)Isocratic 3/7 H2O/MeOH with 0.1 % Formic Acid5 µL injectionFlow rate of 0.8 mL/min

2.8 2.9 3 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8100

200

300

400

500

600

700

800

Time (min)

Inte

nsity

(CPS

)

3Q 300 series

ECI: LC-MRM, Lowest limit of detection for Testosterone (298.497.1)

2.8 2.9 3 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8100

200

300

400

500

600

700

800

Time (min)In

tens

ity (C

PS)

200 ag/μLS/N= 11

LC condition:

Shimadzu Prominence UFLCKinetex C18 column (75x2.1 mm,2.6 µm)8 min gradient from 5 to 95% MeOH in 10 mM NH4OAc and 0.1 % Formic AcidInjection volume:  10 µL Flow rate of 0.5 mL/min

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1 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 20

20

40

60

80

100

120

140

160

180

200

Time (min)

Inte

nsity

(CPS

)

1 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 20

20

40

60

80

100

120

140

160

180

200

Time (min)

Inte

nsity

(CPS

)

3Q 300 series

ESI: LC-MRM, Lowest limit of detection for AVP (542.9120.1) in plasma

2.5 fg/μLS/N= 11

LC condition:

Shimadzu Prominence UFLC model6 min gradient run from 5 to 90% ACN in 0.05 % Formic AcidThermo BetaBasic -C18 column, (30x2.1mm, 3µm)Injection volume:  10µLFlow rate of 0.4 mL/min

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1.2 1.3 1.4 1.5 1.6 1.7 1.80

20

40

60

80

100

120

140

Time (min)

Inte

nsity

(CPS

)

Blank

3Q 300 seriesECI: LC-MRM, Lowest limit of detection for Fentanyl (337.5188.1)

LC condition:

Shimadzu Prominence UFLCxr modelKinetex C18 column (75x2.1 mm, 2.6 µm)5 min gradient from 10 to 95% MeOH in 10 mM NH4OAc and 0.1 % Formic AcidInjection volume:  10 µL Flow rate of 0.5 mL/min

1.2 1.3 1.4 1.5 1.6 1.7 1.80

20

40

60

80

100

120

140

Time (min)

Inte

nsity

(CPS

)

33 ag/μLS/N= 20

3Q 100 3Q 200 3Q 300

Fentanyl (337.1/188)S/N ≈ 7

1.5 fg/uL 250 ag/uL 32 ag/uLX 6 X 8

Reserpine 5 fg/μL S/N=230S/N=32S/N=5 X 6 X 6

Testosterone (289.1/97.1) 10 fg/μLS/N=11

1.25 fg/μLS/N=11

0.25 fg/μLS/N=11

X 8 X 5

IONICS’ Current line of products

IONICS Mass Spectrometry

• Pushing the limits Sensitivity– Micro-dosing?

• Working with Industry Thought Leaders• Innovating at a serious pace - Series 100,

Series 200, Series 300…• Join us

Live long and prosper

Acknowledgements

IONICS Team• Reza Javahery• Lisa Cousins• Chuck Joliffe• Josh Ye• Changtong Hao• Hui Qiao• Ellie Majdi• Alex Taylor• Heather Gamble• George Scott• Brian Clark• Frenny Riparelli