201310 springer mexico 120min

Jeff Robens, PhD Senior Editor

�

Author Academy: Steps to Publica<on Success

Entre Pares 8 October 2013

edanzedi7ng.com/entrepares

About Jeff…

Author

Senior Editor

Peer reviewer

Cell biology

Tissue engineering

Neuroscience

Why publish in English?

ü  Interna<onal language of academics

ü  Build an interna<onal reputa<on ü  Develop interna<onal collabora<ons

ü  Overseas research opportuni<es

1.   Journal selec<on 2.   Manuscript structure

3.   Targe<ng the journal 4.   Effec<ve wri<ng 5.   Cover leOers 6.   Peer review

Today’s presentation …

Edanz survey results – Primary difficulty during manuscript preparation

n = 333 published non-‐na7ve English authors

Choosing a journal

Expressing thoughts clearly in English

Understanding the author guidelines

FormaKng according to the guidelines

118

79

73

63

35.4%

23.7%

21.9%

18.9%

Journal selec3on

Section 1

Journal selec7on Timing

Choose your target journal: – A#er you have decided you have enough results for a publica7on

– A#er you have decided on how high to aim—high, medium or low impact

– A#er you have decided how broadly relevant your findings are

– Before wri7ng the Title, Abstract, Introduc7on or Discussion sec7ons

Journal selec7on Tenga en cuenta los diferentes factores de cada revista

Aims & scope Readership

Open access Indexing

Which factor is most important to you?

Publica<on frequency

Impact factor

Varies by field

Journal selec7on Choosing a target journal

Honestamente evalue la importancia de su inves3gación

Significance Aims and Scope Impact

Journal selec7on Evaluating significance

How new are your findings? Novelty

How broadly relevant are your findings? Relevance

What are the important real-‐world applica<ons? Appeal

Journal selec7on

Insert your proposed abstract

El Journal Selector ayuda a elegir la publicacion más adecuada

Journal Selector – www.edanzediting.com/journal_selector

Journal selec7on

Recommended journals

Filter by: Impact factor

Publishing frequency Open access


Journal selec7on

Seman<c matching terms

Journals IF, Aims & Scope, and Frequency

Similar published ar<cles

Have they published similar ar<cles recently? Have you cited some of these ar<cles?


Journal selec7on Visit journal websites

Journal selec7on Tips to identify the most suitable journal

S

Iden<fy the interests of the journal editor

Iden<fy the interests of the

readers

•  Editorials •  Review ar<cles •  Special issues

•  Most viewed •  Most cited

Iden3fica los intereses del editor de la revista y de los lectores en cada publicacion

Manuscript structure

Section 2

Coverage and Staffing Plan Manuscript structure IMRaD

Ø  Title Ø  Abstract Ø  Introduc7on Ø Methods

Ø  Results Ø  and Ø  Discussion

The beginning

The end

The middle

Coverage and Staffing Plan Manuscript structure

Important points

Summarize key finding Contains keywords Less than 20 words

Avoid

Ques<ons Abbrevia<ons

“New” or “novel”

Effective titles

El Gtulo debe ser conciso y resumir su hallazgos clave

Coverage and Staffing Plan Manuscript structure Abstract

First impression of your paper

Importance of your results

Validity of your conclusions

Relevance of your aims

Judge your wri<ng style

Probably only part that will be read

Coverage and Staffing Plan Manuscript structure Sections of an abstract

Aims

Background

Methods

Results

Conclusion

Why the study was done (20%)

Your hypothesis (10%)

Techniques (10%)

Most important findings (40%)

Conclusion/implica<ons (20%)

Concise summary of your research

Coverage and Staffing Plan Manuscript structure Unstructured abstract

Our understanding of the mechanisms by which ducts and lobules develop is derived from model organisms and three-‐dimensional (3D) cell culture models wherein mammalian epithelial cells undergo morphogenesis to form mul7cellular spheres with a hollow central lumen. However, the mechanophysical proper7es associated with epithelial morphogenesis are poorly understood. We performed mul7dimensional live-‐cell imaging analysis to track the morphogene7c process star7ng from a single cell to the development of a mul7cellular, spherical structure composed of polarized epithelial cells surrounding a hollow lumen. We report that in addi7on to ac7vely maintaining apicobasal polarity, the structures underwent rota7onal mo7ons at rates of 15–20 μm/h and the structures rotated 360° every 4 h during the early phase of morphogenesis. Rota7onal mo7on was independent of the cell cycle, but was blocked by loss of the epithelial polarity proteins Scribble or Pard3, or by inhibi7on of dynein-‐based microtubule motors. Interes7ngly, none of the structures derived from human cancer underwent rota7onal mo7on. We found a direct rela7onship between rota7onal mo7on and assembly of endogenous basement membrane matrix around the 3D structures, and that structures that failed to rotate were defec7ve in weaving exogenous laminin matrix. Dissolu7on of basement membrane around mature, nonrota7ng acini restored rota7onal movement and the ability to assemble exogenous laminin. Thus, coordinated rota7onal movement is a unique mechanophysical process observed during normal 3D morphogenesis that regulates laminin matrix assembly and is lost in cancer-‐derived epithelial cells.

Wang et al. PNAS. 2013; 110: 163‒168.

Coverage and Staffing Plan Manuscript structure Unstructured abstract

Conclusion Thus, coordinated rota7onal movement is a unique mechanophysical process observed during normal 3D morphogenesis that regulates laminin matrix assembly and is lost in cancer-‐derived epithelial cells.

Results

We report that in addi7on to ac7vely maintaining apicobasal polarity, the structures underwent rota7onal mo7ons at rates of 15–20 μm/h and the structures rotated 360° every 4 h during the early phase of morphogenesis. Rota7onal mo7on was independent of the cell cycle, but was blocked by loss of the epithelial polarity proteins Scribble or Pard3, or by inhibi7on of dynein-‐based microtubule motors. Interes7ngly, none of the structures derived from human cancer underwent rota7onal mo7on. We found a direct rela7onship between rota7onal mo7on and assembly of endogenous basement membrane matrix around the 3D structures, and that structures that failed to rotate were defec7ve in weaving exogenous laminin matrix. Dissolu7on of basement membrane around mature, nonrota7ng acini restored rota7onal movement and the ability to assemble exogenous laminin.

Methods We performed mul7dimensional live-‐cell imaging analysis to track the morphogene7c process star7ng from a single cell to the development of a mul7cellular, spherical structure composed of polarized epithelial cells surrounding a hollow lumen.

Background Our understanding of the mechanisms by which ducts and lobules develop is derived from model organisms and three-‐dimensional (3D) cell culture models wherein mammalian epithelial cells undergo morphogenesis to form mul7cellular spheres with a hollow central lumen. However, the mechanophysical proper7es associated with epithelial morphogenesis are poorly understood.

Wang et al. PNAS. 2013; 110: 163‒168.

Escriba su reseña en secciones

Coverage and Staffing Plan Manuscript structure Writing your abstract


Write the results sec<on first

ü  Key findings that directly support your aims ü  Will be interes<ng to the readers

Wang et al. PNAS. 2013; 110: 163‒168.


Our understanding of the mechanisms by which ducts and lobules develop is derived from model organisms and three-‐dimensional (3D) cell culture models wherein mammalian epithelial cells undergo morphogenesis to form mul7cellular spheres with a hollow central lumen. However, the mechanophysical proper7es associated with epithelial morphogenesis are poorly understood.

Write the background sec<on second

ü  Explain why this study needed to be done

Problem

Wang et al. PNAS. 2013; 110: 163‒168.


We performed mul7dimensional live-‐cell imaging analysis to track the morphogene7c process star7ng from a single cell to the development of a mul7cellular, spherical structure composed of polarized epithelial cells surrounding a hollow lumen.

Write the methods sec<on third

ü  General techniques used to obtain the presented results

Wang et al. PNAS. 2013; 110: 163‒168.


Thus, coordinated rota7onal movement is a unique mechanophysical process observed during normal 3D morphogenesis that regulates laminin matrix assembly and is lost in cancer-‐derived epithelial cells.

Write the conclusion sec<on last

ü  Major conclusion that answers the problem ü  Implica<ons for the readers

However, the mechanophysical proper<es associated with epithelial morphogenesis are poorly understood.

Implica<ons

Conclusion

Wang et al. PNAS. 2013; 110: 163‒168.


Thus, coordinated rota7onal movement is a unique mechanophysical process observed during normal 3D morphogenesis that regulates laminin matrix assembly and is lost in cancer-‐derived epithelial cells.


We performed mul7dimensional live-‐cell imaging analysis to track the morphogene7c process star7ng from a single cell to the development of a mul7cellular, spherical structure composed of polarized epithelial cells surrounding a hollow lumen.

Our understanding of the mechanisms by which ducts and lobules develop is derived from model organisms and three-‐dimensional (3D) cell culture models wherein mammalian epithelial cells undergo morphogenesis to form mul7cellular spheres with a hollow central lumen. However, the mechanophysical proper7es associated with epithelial morphogenesis are poorly understood.

Wang et al. PNAS. 2013; 110: 163‒168.


Our understanding of the mechanisms by which ducts and lobules develop is derived from model organisms and three-‐dimensional (3D) cell culture models wherein mammalian epithelial cells undergo morphogenesis to form mul7cellular spheres with a hollow central lumen. However, the mechanophysical proper7es associated with epithelial morphogenesis are poorly understood. We performed mul7dimensional live-‐cell imaging analysis to track the morphogene7c process star7ng from a single cell to the development of a mul7cellular, spherical structure composed of polarized epithelial cells surrounding a hollow lumen. We report that in addi7on to ac7vely maintaining apicobasal polarity, the structures underwent rota7onal mo7ons at rates of 15–20 μm/h and the structures rotated 360° every 4 h during the early phase of morphogenesis. Rota7onal mo7on was independent of the cell cycle, but was blocked by loss of the epithelial polarity proteins Scribble or Pard3, or by inhibi7on of dynein-‐based microtubule motors. Interes7ngly, none of the structures derived from human cancer underwent rota7onal mo7on. We found a direct rela7onship between rota7onal mo7on and assembly of endogenous basement membrane matrix around the 3D structures, and that structures that failed to rotate were defec7ve in weaving exogenous laminin matrix. Dissolu7on of basement membrane around mature, nonrota7ng acini restored rota7onal movement and the ability to assemble exogenous laminin. Thus, coordinated rota7onal movement is a unique mechanophysical process observed during normal 3D morphogenesis that regulates laminin matrix assembly and is lost in cancer-‐derived epithelial cells.

Wang et al. PNAS. 2013; 110: 163‒168.


Our understanding of the mechanisms by which ducts and lobules develop is derived from model organisms and three-‐dimensional (3D) cell culture models wherein mammalian epithelial cells undergo morphogenesis to form mul7cellular spheres with a hollow central lumen. However, the mechanophysical proper7es associated with epithelial morphogenesis are poorly understood. We performed mul7dimensional live-‐cell imaging analysis to track the morphogene7c process star7ng from a single cell to the development of a mul7cellular, spherical structure composed of polarized epithelial cells surrounding a hollow lumen. We report that in addi7on to ac7vely maintaining apicobasal polarity, the structures underwent rota7onal mo7ons at rates of 15–20 μm/h and the structures rotated 360° every 4 h during the early phase of morphogenesis. Rota7onal mo7on was independent of the cell cycle, but was blocked by loss of the epithelial polarity proteins Scribble or Pard3, or by inhibi7on of dynein-‐based microtubule motors. Interes7ngly, none of the structures derived from human cancer underwent rota7onal mo7on. We found a direct rela7onship between rota7onal mo7on and assembly of endogenous basement membrane matrix around the 3D structures, and that structures that failed to rotate were defec7ve in weaving exogenous laminin matrix. Dissolu7on of basement membrane around mature, nonrota7ng acini restored rota7onal movement and the ability to assemble exogenous laminin. Thus, coordinated rota7onal movement is a unique mechanophysical process observed during normal 3D morphogenesis that regulates laminin matrix assembly and is lost in cancer-‐derived epithelial cells. Conclusions

Results

Methods

Background

Wang et al. PNAS. 2013; 110: 163‒168.

Coverage and Staffing Plan Manuscript structure Introduction

General introduc<on

Specific aims Aims

Current state of the field

Problem in the field

Secondary introduc<on

Current state of the field

Problem in the field

Coverage and Staffing Plan Manuscript structure

Introduction – flow of information

Lung cancer is the leading cause of cancer mortality for men and women. Despite smoking preven7on and cessa7on programs and advances in early detec7on, the 5-‐year survival rate for lung cancer is only 16% with current therapies. Although lung cancer incidence rates have recently declined in the United States, more lung cancer is now diagnosed when considered together in former-‐ and never-‐smokers than in current smokers. Thus, even if all of the na7onal an7-‐smoking campaign goals are met, lung cancer will remain a major public health problem for decades. New ways to treat or prevent lung cancer are therefore needed. One poten7al therapeu7c target for lung cancer is the Wnt signaling pathway. The canonical Wnt signaling pathway in mammals consists of a family of secreted lipid-‐modified Wnt protein ligands that bind to a family of 7-‐pass transmembrane Frizzled (Fzd) receptors, as reviewed. In brief, in the absence of ligand, glycogen synthase kinase-‐3 (GSK3), in complex with axin and adenomatous polyposis coli (APC), cons7tu7vely phosphorylates β-‐catenin, the primary Wnt signaling effector, targe7ng it for ubiqui7na7on and proteasomal destruc7on. Ligand binding engages a pathway involving Dishevelled (Dvl) that inhibits GSK3, allowing β-‐catenin to accumulate in a hypophosphorylated form. This stabilized form of β-‐catenin can translocate to the nucleus, where it ac7vates target gene transcrip7on by complexing with T cell factor (TCF) and lymphoid enhancer-‐binding factor (LEF). In addi7on to key mediators of embryonic development, these target genes include cri7cal growth-‐regulators such as myc and cyclin D1. Aberrant Wnt signaling due to muta7ons in β-‐catenin or APC drives deregulated growth in both familial and non-‐hereditary colorectal cancers. However, non-‐small cell lung cancers (NSCLC), the most common type of lung cancer, rarely harbor APC or β-‐catenin muta7ons. Rather, aberrant Wnt ac7vity in lung cancer is linked to increased expression of upstream Wnt signaling effectors such as Dvl or decreased expression of Wnt antagonists such as Wnt-‐inhibitory factor 1 (Wif-‐1). Effec7ve pharmacological inhibitors of the Wnt pathway have only recently become available. Screens for small-‐molecule antagonists of the Wnt pathway found two enzymes to be key mediators of Wnt signaling. These are poly-‐ADP-‐ribose polymerase (PARP) enzymes, tankyrase (TNKS) 1 and TNKS2, which anach poly-‐ADP-‐ribose (PAR) onto substrate proteins. Their roles in regula7ng telomerase func7on and mito7c spindle forma7on are known, but their role in PARsyla7ng axin so as to maintain the op7mal level for canonical Wnt signaling has only recently been recognized. The compounds iden7fied in these screens, XAV939, IWR-‐1 exo, and IWR-‐1 endo, act by specifically inhibi7ng the PARP ac7vity of TNKS1 and TNKS2. IWR-‐exo is a stereoisomer of IWR-‐1 endo with ~14-‐fold lower EC50. PARP inhibi7on is a tractable pharmacological target in vivo, as antagonists of other PARP homologs exert an7neoplas7c responses in breast and ovarian cancer, as reviewed. This study explored the hypothesis that inhibi7on of TNKS by pharmacological or gene7c means would inhibit lung cancer growth in vitro and in vivo in clinically-‐relevant transgenic mouse models of lung cancer that were previously developed, as reviewed.

General introduc<on

Secondary introduc<on

Objec<ves Busch et al. BMC Cancer. 2012; 13: 211.

Coverage and Staffing Plan Manuscript structure Methods

How it was done

General methods Specific techniques (discuss controls)

Data analysis Quan<fica<on methods

Sta<s<cal tests

What was used

Samples or par<cipants Materials

How it was analyzed

Sus métodos reflejan su diseño experimental

Coverage and Staffing Plan Manuscript structure Results

1.   Ini<al observa<on 2.   Characteriza<on 3.   Applica<on

Each subsec<on corresponds to

one figure

What you found, not what it means

Logical presenta<on

Subsec<ons

Factual descrip<on

Presente sus resultados en un orden lógico

Coverage and Staffing Plan Manuscript structure Display items

Present large amount of data quickly and

efficiently

Keep it simple: use separate panels if

necessary

Must be able to stand alone: clear labels and figure legends

Usually the first thing readers will look at

Figures, graphs & tables

Coverage and Staffing Plan Manuscript structure Figures/schematics

Clear indicators

Clear figure legend Kindlin-‐2 knockdown and focal adhesion localiza<on. A. Confocal immunofluorescent microscopy with an7-‐β1 integrin (green) and an7-‐paxillin (red) on C2C12 cells transfected with RNAi and then changed to differen7a7on media for 2 days. Control cells (scr RNAi) show linear staining consistent with localiza7on to costameres (arrows), as well as punctate focal contact staining (arrowheads). Conversely, focal contact proteins in the kindlin-‐2 RNAi cells fail to form linear structures and instead are concentrated in unusual appearing puncta (*). (Scale bar = 20 μM).

Dowling et al. (2008) BMC Cell Biol 9:36.

Coverage and Staffing Plan Manuscript structure Discussion

Beginning

Middle

End

Summarize key findings State major conclusion

Restate major conclusion Applica<ons/implica<ons

Suggest future work

Interpret results in context of other studies

Describe limita<ons

Coverage and Staffing Plan Manuscript structure References

Cite all statements from previously published works

Use reference management sorware EndNote, Papers, RefWorks,

Mendeley

Cite broadly from different groups in your field

Coverage and Staffing Plan Manuscript structure Linking your ideas

General background

Objec<ves

Methodology

Results and figures

Summary of findings

Implica<ons for the field

Relevance of findings

Knowledge gaps

Enlace lógicamente sus ideas en su inves3gación

Current state of the field Introduc<on

Methods

Results

Discussion

Coverage and Staffing Plan Manuscript structure Linking your ideas

New ways to treat or prevent lung cancer are therefore needed.

This study explored the hypothesis that inhibi<on of TNKS…would inhibit lung cancer growth…

Pharmacological or gene<c inhibi<on of TNKS1 and TNKS2…reduces lung cancer prolifera<on...

Problem

Objec<ves

Conclusion

Discussion

Introduc3on

Busch et al. BMC Cancer. 2012;13:211.

Target the journal

Section 3

Customer Service Target the journal

Chlorophyll is present in many plant organs, including immature fruit where it is usually degraded during ripening. Mature green kiwifruit (Ac9nidia deliciosa) are an excep7on, with high concentra7ons of chlorophyll remaining in the fruit flesh. In gold-‐fleshed kiwifruit (A. chinensis), chlorophyll is degraded to colourless catabolites upon fruit ripening, leaving yellow carotenoids visible. We have iden7fied candidate genes for the control of chlorophyll degrada7on…

Pilkington et al. Planta. 2012; 236: 1615−1628.

The control of chlorophyll levels in maturing kiwifruit

Broad focused journal

Planta


Chlorophyll is present in many plant organs, including immature fruit where it is usually degraded during ripening. Mature green kiwifruit (Ac9nidia deliciosa) are an excep7on, with high concentra7ons of chlorophyll remaining in the fruit flesh. In gold-‐fleshed kiwifruit (A. chinensis), chlorophyll is degraded to colourless catabolites upon fruit ripening, leaving yellow carotenoids visible. We have iden7fied candidate genes for the control of chlorophyll degrada7on…

Pilkington et al. Planta. 2012; 236: 1615−1628.

The control of chlorophyll levels in maturing kiwifruit Broad interest

Broad interest

Broad focused journal

Planta


Tradi7onal weed management, such as 7llage and irriga7on, has led to an enhanced maintenance of wetland plant species in fallow paddy fields. Recent herbicide usage and improvements in irriga7on and drainage systems however have caused habitat loss of these species, especially in fields on open lowlands…

Effects of <llage and irriga<on on the occurrence and establishment of na<ve wetland plant species in fallow paddy fields

Narrow focused journal

Paddy and Water Development

Takanose et al. Paddy Water Environ. 2013; 11: 1−4.


Tradi7onal weed management, such as 7llage and irriga7on, has led to an enhanced maintenance of wetland plant species in fallow paddy fields. Recent herbicide usage and improvements in irriga7on and drainage systems however have caused habitat loss of these species, especially in fields on open lowlands…

Effects of <llage and irriga<on on the occurrence and establishment of na<ve wetland plant species in fallow paddy fields

Narrow focused journal

Paddy and Water Development

Takanose et al. Paddy Water Environ. 2013; 11: 1−4.

Keywords from the Aims and Scope

Effec3ve wri3ng

Section 4

Effec7ve Wri7ng High readability

Your reader should…

Understand your logic immediately

Not have to read slowly

Only have to read once

Effec7ve Wri7ng 1. Short sentences

Reading once… 4% of readers can understand a 27-‐word sentence 75% of readers can understand a 17-‐word sentence

Pinner and Pinner (1998) Communica9on Skills

U<lice sólo una idea por oracion 15–20 palabras

Effec7ve Wri7ng Short sentences

30 words

The largest company, a Mexican corpora<on founded in 1916 outside of Puebla by Miguel Hernández, was considered to be a model in the development of modern employee condi<ons by economists.

Effec7ve Wri7ng Short sentences

Economists considered the largest company to be a model in the development of modern employee condi<ons. This company was a Mexican corpora<on founded in 1916 outside of Puebla by Miguel Hernández.

16 words

15 words One idea per sentence

Effec7ve Wri7ng 2. Active voice

Sentences wriOen in the ac<ve voice are:

simple direct clear easy to read

The mechanisms regula<ng tumor growth were inves<gated. Passive

We inves<gated the mechanisms regula<ng tumor growth. Ac3ve

U3lice la voz ac3va para ser más directos

Effec7ve Wri7ng Active voice is preferred

“Use the ac3ve voice when it is less wordy and more direct than the passive”. (3rd ed., pg. 42)

“Use the ac3ve voice rather than the passive voice…”. www.apastyle.org/learn/faqs/effec7ve-‐verb-‐use.aspx

“As a maner of style, passive voice is typically, but not always, inferior to ac3ve voice”. (15th ed., pg. 177)

“In general, authors should use the ac3ve voice…”. (10th ed., pg. 320)

ACS Style Guide

APA Style

Chicago Style Guide

AMA Manual of Style

Effec7ve Wri7ng 3. Stress position

Los lectores se centran en el final de la oracion para iden3ficar lo que es importante

1.   You deserve a raise, but the budget is <ght.

Which sentence suggests that you will get a raise?

2.   The budget is <ght, but you deserve a raise.

hnp://wri7ngcenter.unc.edu/handouts/flow/

Effec7ve Wri7ng Stress position

The budget is <ght, but you deserve a raise. Your salary

will increase at the beginning of next year.

La posición de tensión debe introducir el tema de la siguiente oracio







Stress posi<on






Stress posi<on Topic posi<on

La posición del tema iden3fica de qué trata la oración

Effec7ve Wri7ng 4. Topic position

The pa3ent went to the hospital to see a gastroenterologist. The doctor then performed a series of diagnos3c tests. The results showed the pa<ent suffered from a bacterial infec3on. An3bio3cs were prescribed to treat the infec<on before the pa<ent developed an ulcer.

idea idea idea idea

Topic link

sentence

2.5 <mes more common in English than in Spanish Simpson JM. J Second Lang Writ. 2000; 9: 293–309.

Effec7ve Wri7ng Manuscript structure

Lung cancer is the leading cause of cancer mortality for men and women. Despite smoking preven<on and cessa<on programs… …New ways to treat or prevent lung cancer are therefore needed. One poten<al therapeu<c target for lung cancer is the Wnt signaling pathway. The canonical Wnt signaling pathway in mammals consists of a…

Busch et al. BMC Cancer. 2012; 13: 211.

Effec7ve Wri7ng

Lung cancer is the leading cause of cancer mortality for men and women. Despite smoking preven<on and cessa<on programs… …New ways to treat or prevent lung cancer are therefore needed. One poten<al therapeu<c target for lung cancer is the Wnt signaling pathway. The canonical Wnt signaling pathway in mammals consists of a…

Busch et al. BMC Cancer. 2012; 13: 211.

Topic sentence

Topic sentence Stress sentence

U<lice la posicion de tensión y tema en su escrito de manera lógica al presentar la información

Manuscript structure

Cover lebers

Section 5

Coverage and Staffing Plan Cover leners

Abstract: First impression for readers

Cover leOers are the first impression for the journal editor

Significance Relevance

Level of English Interes<ng to their readers?

Is your work important?

Coverage and Staffing Plan Cover leners Building your cover letter

Marc Lippman, MD Editor-‐in-‐Chief Breast Cancer Research and Treatment 3 September 2013 Dear Dr Lippman, Please find enclosed our manuscript en7tled “Evalua7on of the Glasgow prognos7c score in pa7ents undergoing cura7ve resec7on for breast cancer liver metastases,” which we would like to submit for publica7on as an Original Ar7cle in Breast Cancer Research and Treatment.

Journal editor’s name

Manuscript <tle

Publica<on type

Indique el Gtulo de su escrito y 3po de publicación


The Glasgow prognos7c score (GPS) is of value for a variety of tumours. Several studies have inves7gated the prognos7c value of the GPS in pa7ents with metasta7c breast cancer, but few studies have performed such an inves7ga7on for pa7ents undergoing liver resec7on for liver metastases. Furthermore, there are currently no studies that have examined the prognos7c value of the modified GPS (mGPS) in these pa7ents. The present study evaluated the mGPS in terms of its prognos7c value for postopera7ve death in pa7ents undergoing liver resec7on for breast cancer liver metastases.

Explique por qué su inves<gación deberia de hacerse

ü  Current state of the field ü  Problem researchers are facing


The Glasgow prognos7c score (GPS) is of value for a variety of tumours. Several studies have inves7gated the prognos7c value of the GPS in pa7ents with metasta7c breast cancer, but few studies have performed such an inves7ga7on for pa7ents undergoing liver resec7on for liver metastases. Furthermore, there are currently no studies that have examined the prognos7c value of the modified GPS (mGPS) in these pa7ents. The present study evaluated the mGPS in terms of its prognos7c value for postopera7ve death in pa7ents undergoing liver resec7on for breast cancer liver metastases.

Explique por qué su inves<gación deberia de hacerse

ü  Current state of the field ü  Problem researchers are facing

Introduc<on

Problem

Objec<ves


Resuma sus conclusiones clave

ü  Briefly describe your methodology ü  Summarize your key findings

A total of 318 pa7ents with breast cancer liver metastases who underwent hepatectomy over a 15-‐year period were included in this study. The mGPS was calculated based on the levels of C-‐reac7ve protein and albumin, and the disease-‐free survival and cancer-‐specific survival rates were evaluated in rela7on to the mGPS. Overall, the results showed a significant associa7on between cancer-‐specific survival and the mGPS and carcinoembryonic an7gen level, and a higher mGPS was associated with increased aggressiveness of liver recurrence and poorer survival in these pa7ents.


A total of 318 pa7ents with breast cancer liver metastases who underwent hepatectomy over a 15-‐year period were included in this study. The mGPS was calculated based on the levels of C-‐reac7ve protein and albumin, and the disease-‐free survival and cancer-‐specific survival rates were evaluated in rela7on to the mGPS. Overall, the results showed a significant associa7on between cancer-‐specific survival and the mGPS and carcinoembryonic an7gen level, and a higher mGPS was associated with increased aggressiveness of liver recurrence and poorer survival in these pa7ents.

ü  Briefly describe your methodology ü  Summarize your key findings

Methods

Key results

Resuma sus conclusiones clave


This study is the first to demonstrate that the preopera7ve mGPS, a simple clinical tool, is a useful prognos7c factor for postopera7ve survival in breast cancer pa7ents undergoing cura7ve resec7on for liver metastases. This informa7on is immediately clinically applicable for surgeons and medical oncologists trea7ng such pa7ents. As a premier journal covering breast cancer treatment, we believe that Breast Cancer Research and Treatment is the perfect plazorm from which to share our results with all those concerned with breast cancer.

Explique por qué su escrito sería interesante para los lectores de una revista



Explique por qué su escrito sería interesante para los lectores de una revista

Conclusion

Relevance



Use palabras clave a par<r de los obje<vos y el alcance en su carta de presentación

Keywords from the Aims and Scope


We confirm that this manuscript has not been published elsewhere and is not under considera7on by another journal. All authors have approved the manuscript, including authorship and order of authorship, and agree with submission to Breast Cancer Research and Treatment. This study was funded by the Ministry of Health, Labour and Welfare. The authors have no conflicts of interest to declare.

Exponga responsabilidad legal sobre é<ca de la inves<gación y la publicación

ü  Disclaimers related to publica3on ethics ü  Sources of Funding ü  Conflicts of interest


We confirm that this manuscript has not been published elsewhere and is not under considera7on by another journal. All authors have approved the manuscript, including authorship and order of authorship, and agree with submission to Breast Cancer Research and Treatment. This study was funded by the Ministry of Health, Labour and Welfare. The authors have no conflicts of interest to declare.

Exponga responsabilidad legal sobre é<ca de la inves<gación y la publicación

ü  Disclaimers related to publica3on ethics ü  Sources of Funding ü  Conflicts of interest

Funding

Ethics

Conflicts of interest


We would like to recommend the following reviewers to evaluate our manuscript: 1.  Reviewer 1 and contact informa7on 2.  Reviewer 2 and contact informa7on 3.  Reviewer 3 and contact informa7on 4.  Reviewer 4 and contact informa7on Please address all correspondence to:

ü  Recommended reviewers ü  Author’s contact informa7on

Other important informa<on:


We would like to recommend the following reviewers to evaluate our manuscript: 1.  Reviewer 1 and contact informa7on 2.  Reviewer 2 and contact informa7on 3.  Reviewer 3 and contact informa7on 4.  Reviewer 4 and contact informa7on Please address all correspondence to:

Recommend reviewers

Other important informa<on:

ü  Recommended reviewers ü  Author’s contact informa7on

Contact informa<on

Coverage and Staffing Plan Cover leners A good cover letter

Marc Lippman, MD Editor-‐in-‐Chief Breast Cancer Research and Treatment 3 September 2013 Dear Dr Lippman, Please find enclosed our manuscript en7tled “Evalua7on of the Glasgow prognos7c score in pa7ents undergoing cura7ve resec7on for breast cancer liver metastases,” which we would like to submit for publica7on as an Original Ar7cle in Breast Cancer Research and Treatment. . The Glasgow prognos7c score (GPS) is of value for a variety of tumours. Several studies have inves7gated the prognos7c value of the GPS in pa7ents with metasta7c breast cancer, but few studies have performed such an inves7ga7on for pa7ents undergoing liver resec7on for liver metastases. Furthermore, there are currently no studies that have examined the prognos7c value of the modified GPS (mGPS) in these pa7ents. The present study evaluated the mGPS in terms of its prognos7c value for postopera7ve death in pa7ents undergoing liver resec7on for breast cancer liver metastases. A total of 318 pa7ents with breast cancer liver metastases who underwent hepatectomy over a 15-‐year period were included in this study. The mGPS was calculated based on the levels of C-‐reac7ve protein and albumin, and the disease-‐free survival and cancer-‐specific survival rates were evaluated in rela7on to the mGPS. Prognos7c significance was retrospec7vely analyzed by univariate and mul7variate analyses. Overall, the results showed a significant associa7on between cancer-‐specific survival and the mGPS and carcinoembryonic an7gen level, and a higher mGPS was associated with increased aggressiveness of liver recurrence and poorer survival in these pa7ents. This study is the first to demonstrate that the preopera7ve mGPS, a simple clinical tool, is a useful prognos7c factor for postopera7ve survival in breast cancer pa7ents undergoing cura7ve resec7on for liver metastases. This informa7on is immediately clinically applicable for surgeons and medical oncologists trea7ng such pa7ents. As a premier journal covering breast cancer treatment, we believe that Breast Cancer Research and Treatment is the perfect plazorm from which to share our results with all those concerned with breast cancer. We confirm that this manuscript has not been published elsewhere and is not under considera7on by another journal. All authors have approved the manuscript, including authorship and order of authorship, and agree with submission to Breast Cancer Research and Treatment. This study was funded by the Ministry of Health, Labour and Welfare. The authors have no conflicts of interest to declare. We would like to recommend the following reviewers to evaluate our manuscript: Reviewer 1 and contact informa7on Reviewer 2 and contact informa7on Reviewer 3 and contact informa7on Reviewer 4 and contact informa7on Please address all correspondence to: We look forward to hearing from you at your earliest convenience. Yours sincerely,

Coverage and Staffing Plan Cover leners A good cover letter

Marc Lippman, MD Editor-‐in-‐Chief Breast Cancer Research and Treatment 3 September 2013 Dear Dr Lippman, Please find enclosed our manuscript en7tled “Evalua7on of the Glasgow prognos7c score in pa7ents undergoing cura7ve resec7on for breast cancer liver metastases,” which we would like to submit for publica7on as an Original Ar7cle in Breast Cancer Research and Treatment. . The Glasgow prognos7c score (GPS) is of value for a variety of tumours. Several studies have inves7gated the prognos7c value of the GPS in pa7ents with metasta7c breast cancer, but few studies have performed such an inves7ga7on for pa7ents undergoing liver resec7on for liver metastases. Furthermore, there are currently no studies that have examined the prognos7c value of the modified GPS (mGPS) in these pa7ents. The present study evaluated the mGPS in terms of its prognos7c value for postopera7ve death in pa7ents undergoing liver resec7on for breast cancer liver metastases. A total of 318 pa7ents with breast cancer liver metastases who underwent hepatectomy over a 15-‐year period were included in this study. The mGPS was calculated based on the levels of C-‐reac7ve protein and albumin, and the disease-‐free survival and cancer-‐specific survival rates were evaluated in rela7on to the mGPS. Prognos7c significance was retrospec7vely analyzed by univariate and mul7variate analyses. Overall, the results showed a significant associa7on between cancer-‐specific survival and the mGPS and carcinoembryonic an7gen level, and a higher mGPS was associated with increased aggressiveness of liver recurrence and poorer survival in these pa7ents. This study is the first to demonstrate that the preopera7ve mGPS, a simple clinical tool, is a useful prognos7c factor for postopera7ve survival in breast cancer pa7ents undergoing cura7ve resec7on for liver metastases. This informa7on is immediately clinically applicable for surgeons and medical oncologists trea7ng such pa7ents. As a premier journal covering breast cancer treatment, we believe that Breast Cancer Research and Treatment is the perfect plazorm from which to share our results with all those concerned with breast cancer. We confirm that this manuscript has not been published elsewhere and is not under considera7on by another journal. All authors have approved the manuscript, including authorship and order of authorship, and agree with submission to Breast Cancer Research and Treatment. This study was funded by the Ministry of Health, Labour and Welfare. The authors have no conflicts of interest to declare. We would like to recommend the following reviewers to evaluate our manuscript: Reviewer 1 and contact informa7on Reviewer 2 and contact informa7on Reviewer 3 and contact informa7on Reviewer 4 and contact informa7on Please address all correspondence to: We look forward to hearing from you at your earliest convenience. Yours sincerely,

Manuscript informa<on

Background

Key findings

Relevance Disclaimers

Recommended reviewers

Coverage and Staffing Plan Cover leners Recommending

reviewers

“When submiKng your paper, you must provide the names, affilia7ons, and valid e-‐mail addresses of five (5) reviewers. If you do not do so, your paper will be returned, unreviewed.”

“Authors are requested to provide the names and full addresses (including e-‐mail address) of up to four poten7al referees…”

Verifique las directrices de la revista para determinar cuántos revisores se recomiendan

Coverage and Staffing Plan Cover leners Recommending

reviewers

Where to find them?

From your reading/references, networking at conferences

How senior? Aim for mid-‐level researchers

Who to avoid? Collaborators (past 5 years), researchers from same ins<tu<on

Interna3onal list: 1 or 2 from Asia, 1 or 2 from Europe, and 1 or 2 from the Americas

Recomiende inves3gadores de nivel medio de diferentes países

Peer Review

Section 6

Peer Review Improves your manuscript

Get involved in the peer review process

La revisión por pares es un proceso posi<vo que mejorará su manuscito

Peer Review What reviewers are looking for

The science

The manuscript

ü  Relevant hypothesis ü  Good experimental design ü  Appropriate methodology ü  Good data analysis ü  Valid conclusions

ü  Logical flow of informa<on ü  Manuscript structure and formazng ü  Appropriate references ü  High readability

Peer Review

Response leOer

Responda claramente a cada comentario de sus revisores

Easy to see changes

Refer to line and page numbers

Use a different color font

Highlight the text

Revision

Peer Review Writing a response letter

Marc Lippman, MD Editor-‐in-‐Chief Breast Cancer Research and Treatment 3 September 2013 Dear Dr Lippman, Re: Resubmission of manuscript reference No. WJS-‐07-‐5739 Please find anached a revised version of our manuscript originally en7tled “Evalua7on of the Glasgow prognos7c score in pa7ents undergoing cura7ve resec7on for breast cancer liver metastases,” which we would like to resubmit for considera7on for publica7on in the Breast Cancer Research and Treatment. The reviewer’s comments were highly insighzul and enabled us to greatly improve the quality of our manuscript. In the following pages are our point-‐by-‐point responses to each of the comments. Revisions in the manuscript are shown as underlined text. In accordance with the first comment, the 7tle has been revised and the en7re manuscript has undergone substan7al English edi7ng. We hope that the revisions in the manuscript and our accompanying responses will be sufficient to make our manuscript suitable for publica7on in the Breast Cancer Research and Treatment.

Address editor personally

Manuscript ID number

Thank reviewers

Highlight major changes

Peer Review Agreeing with reviewers

Reviewer Comment: In your analysis of the data you have chosen to use a somewhat obscure fiGng func9on (regression). In my opinion, a simple Gaussian func9on would have sufficed. Moreover, the results would be more instruc9ve and easier to compare to previous results.

Response: We agree with the reviewer’s assessment of the analysis. Our tailored func7on, in its current form, makes it difficult to tell that this measurement cons7tutes a significant improvement over previously reported values. We describe our new analysis using a Gaussian fiKng func7on in our revised Results sec7on (Page 6, Lines 12–18).

Peer Review


Response: We agree with the reviewer’s assessment of the analysis. Our tailored func7on, in its current form, makes it difficult to tell that this measurement cons7tutes a significant improvement over previously reported values. We describe our new analysis using a Gaussian fiKng func7on in our revised Results sec7on (Page 6, Lines 12–18).

Agreeing with reviewers

Agreement

Revisions Loca<on

Peer Review Disagreeing with reviewers


Response: Although a simple Gaussian fit would facilitate comparison with the results of other studies, our tailored func7on allows for the analysis of the data in terms of the Smith model [Smith et al., 1998]. We have now explained the use of this func7on and the Smith model in our revised Discussion sec7on (Page 12, Lines 2–6).

Peer Review


Response: Although a simple Gaussian fit would facilitate comparison with the results of other studies, our tailored func7on allows for the analysis of the data in terms of the Smith model [Smith et al., 1998]. We have now explained the use of this func7on and the Smith model in our revised Discussion sec7on (Page 12, Lines 2–6).

Disagreeing with reviewers

Revisions

Loca<on

Evidence

Peer Review

Reviewer comment: The authors looked for polymorphisms in the promoter region of the gene; however, they didn't evaluate the untranslated regions. That is one of my concerns about this methodology.

“Hidden” questions

Rephrased ques3on: Why didn’t the authors evaluate polymorphisms in the untranslated regions of the gene?

Peer Review



Response: In this study, we decided to focus on the promoter region of this gene because previous studies [Yajima et al., 2010; Jackson et al., 2011] have shown that its transcrip7on was par7cularly affected. This has now been clarified in the Discussion sec7on of our manuscript (Page 16, Line 24–28).

Peer Review



Response: In this study, we decided to focus on the promoter region of this gene because previous studies [Yajima et al., 2010; Jackson et al., 2011] have shown that its transcrip7on was par7cularly affected. This has now been clarified in the Discussion sec7on of our manuscript (Page 16, Line 24–28).

Revisions

Loca<on

Evidence

Summary ü Advantages of publishing in English ü Choosing the most suitable journal

ü Logically presen<ng informa<on in your manuscript

ü Clearly communica<ng your ideas in English

ü Communica<ng with journal editors

ü Naviga<ng peer review

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201310 springer mexico 120min

Health & Medicine

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