2013 skin and wound infections_ student.ppt
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2013 Skin and wound infections_ student.pptTRANSCRIPT
04/11/23
SKIN AND WOUND INFECTIONS
Dr. Lakmini Yapa, Senior Registrar ( Medical Microbiology),
T/H Kurunegala
1. Describe the risk factors for infections of the skin
2. Describe the principles of classifying post operative wound infections
3. Describe the methods of collection and transport of samples for microbiological diagnosis
4. Outline principles of treatment and prevention
Be able to
Factors controlling skin microbial load
Dry skinFew organisms
Mainly staphylococci /diphtheroids
Moist skinMany organisms
Including staphylococci /Diphtheroids/
Gram negatives etc
pustule
papilloma
macule
vesicleulcer
Wound Depth can Result in Different Diseases
S. aureus infections
S. aureus infections
Folliculitis
Carbuncle
•CA MRSA•Near nares / upper lips -
S. aureus infections
Group A Streptococcal
infections
cellulitis
Rash of scarlet fever
lymphadenitisNecrotizing fasciitis
erysepalas
Necrotizing fasciitis
• Clinical features– Early – pain, cellulitis, fever,
swelling, induration, skin anaesthesia
– Late – severe pain, blistering, skin discolouratin, dishwater discharge, crepitus, multi-organ failure
• Diagnosis– Clinical and needs high index
of suspicion
• Microbiology• Management
Necrotizing fasciitis
• Microbiology– Group A streptococci – 15% of cases
• M types 1 & 3• Produce exotoxin A and streptolysin O which act as
super-antigens
– Often polymicrobial• Other Gram positive cocci• Gram negative bacilli – aeobic and anaerobic
– Occasionally fungi and vibrio
• Management– Early diagnosis and adequate surgical
debridement– Appropriate antibiotics
Cutaneous infections
Cutaneous anthrax
ErythrasmaCorynebacterium minutissimum
Mycobacterial infections
Atypical mycobacterial infections
M ulcerans
M.marinum
Buruli ulcer
Viral infections of skin
fungal infections of the skin
Tinea versicolor onychomycosis
Candida infection
Serious infections associated with
skin manifestations
Post operative wound infections
• Increases morbidity / mortality
• Increases cost
• Varying risk– Type of operation– Elective / emergency– Experience of surgeon– Patient co-morbidities
Classification of operative wounds by level of bacterial
contamination
Classification of operative wounds by level of bacterial
contamination
• Clean wound – in which no inflammation was encountered– a non traumatic wound – no break in technique occurred – respiratory/ GU and alimentary tracts not opened
I. Vascular surgery
II. mastectomy
infection rate: without prophylaxis 5.1% with prophylaxis 0.8%
• Clean-contaminated wound – non traumatic
– GI/GU/Respiratory tract entered without spillageI. Appendectomy
II. Abdominal and Vaginal Hysterectomy
Classification of operative wounds by level of bacterial
contamination
Classification of operative wounds by level of bacterial
contamination
Infection rate: without prophylaxis 10.1% with prophylaxis 1. 3%
• Contaminated wound– fresh traumatic wound from a relatively clean source– operative wound with major breach in technique– gross spillage from GI tract– entry into biliary or urinary tract with infected bile or urine
• incisions encountering acute non purulent inflammation• dirty wounds (dirty source/delayed)• faecal contamination / foreign bodies / devitalized viscus• pus from any source encountered
Classification of operative wounds by level of bacterial
contamination
Classification of operative wounds by level of bacterial
contamination
Infection rate: without prophylaxis 21.9% with prophylaxis
10.2%
Classification of operative wounds by level of bacterial contamination
• Dirty wound– Operative wound dirty – Traumatic wound from dirty source – Traumatic wound with delayed treatment – Fecal contamination – Foreign body – Retained devitalized tissue – Operative wound w/ acute bacterial inflammation or perforated viscus – Operative wound where clean tissue is transected to gain access to a
collection of pus
Major pathogens
• Clean surgery - S aureus• gut related surgery - mixed flora including
anaerobes
•Specific pathogen not reliably predictable
•Sensitivities also not reliably predictable
•Differentiate between colonization and pathogen necessary
•multiple pathogens common in many situations (gut)
•antibiotic cover for major pathogen(s) adequate - both for prophylaxis and treatment)
Route of bacterial contamination of surgical wounds
Prevention of surgical infections
• Surgical technique
• Asepsis
• Antibiotic prophylaxis
Microbial concentration and virulence
+Injury to wound tissue
+Foreign material
Resistance to perioperative antibiotics
+
=
Risk of wound infections
General and host immunity
+ Peri-operative antibiotics
Interventions of benefit in reducing risk of surgical wound infections
• Reduce inoculation of virulent or antimicrobial resistant bacteria into wound
Interventions of benefit in reducing risk of surgical wound infections (cont.)
• Reduce inoculation of virulent or antimicrobial resistant bacteria into wound– Intra-operative and post-operative
• careful preparation of skin with povidone-iodine or chlorhexidine containing solution
• rigorous adherence to aseptic technique
• high flow of filtered air*
• consider laminar flow environment*
• consider irrigation of wound with antibiotic containing solution
• isolate clean from contaminated surgical fields
• minimize drains (drains through separate stab wound)
• minimize catheters and IV lines post-operatively
Peri-operative antibiotic prophylaxis• Efficacy - unquestioned• Selection of appropriate antibiotic(s)
– based on clinical trials (usually under powered)– likely pathogen(s) and ABST– safety / cost
• Timing and duration of prophylaxis– with induction (exception-colonic surgery)– operations>4 hours - repeat dose intra-operatively every 4 hours– duration -contentious issue (single vs multiple)
Peri-operative antibiotic prophylaxis (cont.)
• Recommendation for prophylaxis– see current literature
• Side effects of prophylaxis– antibiotic associated colitis– vancomycin related hypotension– selection of resistant ‘hospital’ flora
• Cost-benefit of prophylaxis– difficult
Aetiological diagnosis in skin infections
Specimens• Macules / papules - usually no direct examination
• Vesicles / ulcers – aspirate / scraping from base
• Pustules/boils / carbuncles – pus (by aspiration or incision and drainage)
• Wound infections – aspirate / tissues removed at surgery (surface swabs often taken but often reflect colonizing flora)
Request from microbiology laboratory
• Microscopy - Gram stain / Zeihl Neelsen / wet prep
• Culture – bacterial / fungal / viral
• Particularly viral infections – antigen detection / PCR
Interpretation of results
Culture of wounds :• Surface of wounds colonized with many organisms• Surface swab reflects this colonization
– miss true pathogen– lead to overuse of antibiotics
http://jdfc.org/spotlight/diabetic-foot-infections-current-diagnosis-and-treatment/
The Journal of Diabetic Foot Complications, 2012; Volume 4, Issue 2, No. 1, Pages 26-45