Full title of paper: A comparison study of Agrobacterium-mediated transformation methods for root-specific promoter analysis in soybean

Journal name: Plant Cell Reports (PCR)

Authors name: Caifeng Li, Haiyan Zhang, Xiurong Wang* and Hong Liao

Address: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Root Biology Center, South China Agricultural University, Guangzhou 510642, China

E-mail address of correspondence author: xrwang@scau.edu.cn

Online Resource 1. Histochemical analysis of transgenic hairy roots containing ProGmPAP21:GUS fusions using different vectors and transformation methods under both LP and HP conditions. Soybean seeds were treated with LP (5 µmol/L) or HP (250 µmol/L) from seed germination. The two pictures in each panel represent partially stained root segments and root tips.

200µm

pCAMBIA3301-GmPAP21:GUS pTF102-GmPAP21:GUS

In vitro In vivo In vitro In vivo

HP

LP

pCAMBIA3301-GmPAP21:GUS pTF102-GmPAP21:GUS

In vitro In vivo In vitro In vivo

HP

LP

HP

LP