2009 nmvb conference nyu dept of cardiothoracic surgery nyu dept of pathology friday december 4 th,...
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2009 NMVB Conference2009 NMVB Conference
NYU Dept of Cardiothoracic SurgeryNYU Dept of Pathology
Friday December 4th, 2009
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Personnel @ NYUPersonnel @ NYU• Dept of Cardiothoracic Surgery:
• Harvey Pass, MD (surgeon, NYU steering committee member & PI)
• Audrey Sorensen, RN (research nurse coordinator)
• Samantha Lincoln (data manager)
• Ryan Harrington (lab technician, thoracic lab)
• Angela Pittman (research project coordinator)
• Dept of Pathology:• Jonathan Melamed, MD (pathologist, NYU steering committee member)
• Jacinta Small (lab technician, pathology lab)
• Tian He (research coordinator; previously Timothy Jolis)
• Xiangtian Kong (pathologist, pathology review)
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2008-20092008-2009• Logistic improvements:
• Streamlined specimen/case-notification process
• Refined tissue procurement process with
response team and procurement kits
• Synchronized local data
with MVB database
• Progression on 5-year plans:
• Frozen TMA
• NYU MVB Paraffin TMA 1
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Prospective cases: 63Retrospective cases: 157 Total # of cases: 220
YearRetrospective
CasesProspective
CasesOverall Total
2007
157 31 188
2008
0 21 21
2009
0 11 11
Total: 220
Mesothelioma AccrualMesothelioma Accrual
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Tissue Collected Tissue Collected (research blocks)(research blocks)
Collection Method # of Blocks / Samples of Frozen Tissue
OCT 124
FOIL 130
Paraffin Blocks 288
Year New cases collected
Collection Method
Frozen tissue only
Paraffin + Frozen tissue
Paraffin tissueonly
2008 7 0 6 1
2009 9 2 7 0
• Emphasis on accruing extrapleural pneumonectomy cases
Meso accrual data (cont’d)Meso accrual data (cont’d)
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Progression on 5 yr plans-Frozen TMAProgression on 5 yr plans-Frozen TMA
Frozen TMA
Frozen Array mold kit
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Frozen TMAFrozen TMA
Normal lung tissue
Tumor tissue (mesothelioma)
MVB #s indicatedistinct patients
N=13 ptsmvb22
21mvb22
21
mvb2189
mvb2215
mvb2219
mvb2189
mvb2215
mvb2219
mvb2190
mvb2191
mvb2197
mvb2190
mvb2191
mvb2197
mvb2217
mvb2214
mvb2199
mvb2217
mvb2214
mvb2199
mvb2203
mvb2211
mvb2213
mvb2203
mvb2211
mvb2213
mvb2212
mvb2212
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Frozen TMA: Equipment & AccessoriesFrozen TMA: Equipment & Accessories
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• NYU MVB Paraffin TMA 1NYU MVB Paraffin TMA 1• Design Phase:
• N= cases/ Block X 4 B• 4 TMA Blocks (same cases on each
block)• Control tissues, cell lines, frozen-
to-formalin tissue (adv: standardizedprocessing)
• Construction Phase: MTA II• Microtomy: Limited number of sections;
histocollimator• Storage: In desiccators under nitrogen
atmosphere• High resolution scan of slide for website
Plans for Paraffin TMAPlans for Paraffin TMA
Sample paraffin TMA
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Paraffin TMA: Equipment & AccessoriesParaffin TMA: Equipment & Accessories
Desiccators
Tissue Micro-Arrayer
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Proposed map for Paraffin TMAProposed map for Paraffin TMA
Cell Lines
Tumor
Control
Liver
Column →
Row ↓ 0 1 2 3 4 5 6 7 8 9 10 11 12 13A LiverB C D E F G H I J K L MN O P Q R
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Special Thanks to…Special Thanks to…
Today’s conference attendeesToday’s conference attendees
And the entire NMVB teamAnd the entire NMVB team