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Workshop on Flow Cytometry Basics 2 Days/3 Hands-on Sessions
7ᵗ�-8ᵗ� & 10ᵗ�-11ᵗ� March, 2015
low cytometer’s ability to simultaneously measure and
Fanalyze multiple physical characteristics of single
particles, is one of the significant features enabling its
current widespread use in both research and clinical se�ings.
With flow cytometry, researchers can analyse cells from different
origins- plants, animals and microbes; finding applications in the
study of apoptosis, characterisation of heterogeneity,
immunophenotyping, functional studies, DNA content, ploidy
analysis, and others. Considering the need for well-trained
employees and research groups in corporates and academic
institutes alike, we at Stellixir Biotech Pvt. Ltd. in collaboration
with Cytometry Solutions Pvt. Ltd., offer training programs and
workshops in flow cytometry, ranging from basic to advanced
modules. Our experts have years of experience in conducting
advanced flow cytometry-based research and workshops, with a
proven track record in the field.
The module ‘Flow Basics’ is intended for beginners and/or
students with limited experience in flow cytometry. It will give
you a solid foundation in the flow cytometry basics, with clear
understanding of the principles underlying the technology. It
will give you an overview of various applications of flow, and
helps you develop project ideas involving flow cytometry. Three
practical sessions are included.
Dates (Twice in a Row in Mar)
7ᵗ�-8ᵗ� & 10ᵗ�-11ᵗ� March, 2015
Speakers/Demonstrators
Dr.Sanjaya Kumar Mallick, PhD- Director & Chief Scientist, Cytometry Solutions Pvt. Ltd.
Mr.Janardhana PB, M.Sc., M.Res. in Oncology- Founder & CEO, Stellixir Biotech Pvt. Ltd.
Eligibility
Graduate/post-graduate students, doctoral/post-doctoral scholars, teaching faculty, academicians, and non-academicians.
Venue
Stellixir Biotech Pvt. Ltd.
38/1A, 2�ᵈ Floor, 8ᵗ� Mile, Nelagadaranahalli Cross,
Opp. Swathi Petrol Bunk,
T. Dasarahalli, Bengaluru-560 057
To Apply
Please e-mail the below mentioned documents to [email protected]
1. Resume with contact details
2. Previous experience with flow
cytometry
Application Deadline
26ᵗ� Feb, 2015
Selection Announcement
2�ᵈ & 3�ᵈ Mar, 2015
Registration & Payment
Graduates/Post-graduates - 6,000 INR
PhDs/PDFs/Faculty - 8,000 INR
Non-academicians - 12,000 INR
You can pay via cash/cheque/DD
Registration Deadline
5ᵗ� Mar, 2015
Meals
Lunches will be provided, as will tea and coffee
www.stellixir.com | [email protected] | +9180 6456 6678 | +91 7022 010 662
Organised By
General Enquiries: +91-7022 010 662 | [email protected]
Balia
Kolkata-700 084
Westbengal. India.
Web: cytometrysolutions.vpweb.in
nd th38/1A, 2 Floor, 8 Mile,
Nelagadaranahalli Cross,
Opp. Swathi Petrol Bunk,
T. Dasarahalli, Bengaluru-560 057
Program Outline
Ÿ Introduction to flow cytometry and its applications
Ÿ Overview of multicolour design and compensation
Ÿ Introduction to data analysis, interpretation and data presentation
Ÿ 3 hands-on sessions (i) Instrument set-up (ii) Cell Cycle Analysis (iii) CD4 Analysis
Program Schedule
Flow Basics (2-days Workshop With 3 Hands-on Sessions)
Day 1
Time Session9.30-11.30 Lecture: Introduction to flow cytometry-Fluidics, optics, and lasers
Detectors and signal processing
Applications of flow cytometer
11.30-11.45
11.45-13.00
13.00-14.00
14.00-15.30
Coffee/tea break
Lecture: Multicolour experiment design
Choice of right fluorochromes and antibodies
Spectral overlap
Voltage se�ings
Importance of experiment controls
Lunch
Lecture: Compensation in multicolour assays- Significance of
compensation and how to perform it
Coffee/tea break
Lecture: Introduction to data analysis, interpretation and reporting
15.30-15.45
15.45-17.30
Day 2
Time Session9.30-10.00 Review of day-1
10.00-11.15
11.15-11.30
11.30-12.15
12.15-13.00
Practical Demonstration: System walk around, including routine checks
of performance – amplifier linearity, sensitivity
Coffee/tea break
Practical Demonstration: Linear analysis and doublet discrimination -
using cell cycle as an example
Practical Demonstration: Multiparameter labelling – fluorescence
compensation (Beads and then peripheral blood lymphocytes – direct
conjugates – two and three colours– colour compensation)
Lunch
Training feedback and valedictory
13.00-14.00
17.15-17.30
Hands-on 2: Cell cycle analysis15.00-16.00
Hands-on 1: Instrument set-up, calibration, and compensation se�ings14.00-15.00
Hands-on 3: Immunophenotying (CD 4 analysis)16.15-17.15
Please Note: The timetable is only an approximate guideline
www.stellixir.com | [email protected] | +9180 6456 6678 | +91 7022 010 662
16.00-16.15 Coffee/tea break