Biodistribution of Biodistribution of SPIO in ApoE SPIO in ApoE

Deficient MiceDeficient MiceCNS-1-1d is MION made CNS-1-1d is MION made

by Dr. Chanby Dr. Chan

3 apoE deficint Mice

1mMol/kg SPIO injected intravenously

Sacrifice 2 hours, one day & two days after injection

Staining of samples from aorta, heart, liver, lung,Spleen and kidney with Perl’s, H&E, CD68

1 Day After Injection, 1 Day After Injection, Aortic Aortic RootRoot

H&E

1 Day After Injection, 1 Day After Injection, Aortic Aortic RootRoot

Perl’s

1 Day After Injection, 1 Day After Injection, Aortic Aortic RootRoot

1 Day After Injection, 1 Day After Injection, Ascending Ascending Aorta Aorta

1 Day After Injection, 1 Day After Injection, Ascending Ascending AortaAorta

Day3 Day3 Aortic RootAortic Root

LiverLiver

Day2

Day3

Day3 Day3 Descending aorta, H&EDescending aorta, H&E

Day3 Day3 Descending aorta, no plaque no Descending aorta, no plaque no IronIron

We have different kinds of SPIO particles that We have different kinds of SPIO particles that we are going to compare and see which one has we are going to compare and see which one has optimal characteristics of macrophage uptake.optimal characteristics of macrophage uptake.

We are doing macrophage uptake studies and We are doing macrophage uptake studies and our preliminary results show that these particles our preliminary results show that these particles are taken up by macrophageare taken up by macrophage