101 lab 6 photosynthesis tutor template 2011.pdf
TRANSCRIPT
-
8/14/2019 101 Lab 6 photosynthesis Tutor template 2011.pdf
1/14
-
8/14/2019 101 Lab 6 photosynthesis Tutor template 2011.pdf
2/14
Plants are important!!!
Nearly everything on the planet depends on energyproduced by photosynthesis
Produces the O 2 in theatmosphere
PhotoautotrophsProduce energy through photosynthesis (plants, algae,bacteria)
-
8/14/2019 101 Lab 6 photosynthesis Tutor template 2011.pdf
3/14
Why leaves are green..is due to the interaction of light with thechloroplasts
The electromagnetic spectrum
-
8/14/2019 101 Lab 6 photosynthesis Tutor template 2011.pdf
4/14
Photosynthesis equation
Reactants Products
6CO2 + 6H2O C6H12O6 + 6O2light
Photosynthesis is split into two distinct reactions
Light reactions photo
Dark reactions synthesis Calvin cycle
Tracking atoms through photosynthesis
-
8/14/2019 101 Lab 6 photosynthesis Tutor template 2011.pdf
5/14
The location of photosynthesis
Chloroplasts
Leaf cross section
Overview of photosynthesis (1)H2O is oxidised
-
8/14/2019 101 Lab 6 photosynthesis Tutor template 2011.pdf
6/14
Overview of photosynthesis (2)
Overview of photosynthesis (3)
-
8/14/2019 101 Lab 6 photosynthesis Tutor template 2011.pdf
7/14
What molecule absorbs light?
Chlorophyll
Chlorophyll is
located in thethylakoidmembrane
Chlorophyll absorption spectrum
Evidence thatchloroplast
pigments areinvolved in
p o osyn es s
-
8/14/2019 101 Lab 6 photosynthesis Tutor template 2011.pdf
8/14
How a photosystem harvests lightPrimaryelectronacceptor
Reactioncenterchlorophyll
eac onCenter
Electron transfer
Photosystem
Pigment molecules
Photosystems and electronsThere are two photosystems PSI and PSII
cyclic
Acyclic involves PSIIand PSI
Cyclic involves PSI only
PSII
PSIe -
e -
PSII
PSI
-
8/14/2019 101 Lab 6 photosynthesis Tutor template 2011.pdf
9/14
How non-cyclic electron flow generates ATP and NADPH
5
Primary e - acceptor6
Fd
H2O
2 O2
r mary e - accep or
2e -1
Pq
Cytochromecomplex
Pc4e - flow provides P700
2e -
-
NADP+reductase
NADP+ NADPH
Photosystem II
P680
Light
energy orsynthesis of
ATP Photosystem I Light
Mechanical analogy..
-
8/14/2019 101 Lab 6 photosynthesis Tutor template 2011.pdf
10/14
Cyclic electron flow just generates ATP
Primary e - acceptor
Fd
H2O
O2
r mary e - accep or
2e -
Pq
Cytochromecomplex
Pce - flow provides P700
2e -
2e -
NADP+reductase
NADP+ NADPH
Fd
Photosystem II
P680
Light
energy orsynthesis of
ATP Photosystem I Light
Todays lab.Measuring the initial velocity of acyclic electron flow
INSTEAD of NADP + as an electron acceptor we areusing the dye DCPIP (pg 6.1 & pg 6.2)
Use class results to determine the relationshipbetween electron flow and light intensity
Sharing specs again READ INSTRUCTIONS
Table on pg 6.3 unknown vol = 120ul
-
8/14/2019 101 Lab 6 photosynthesis Tutor template 2011.pdf
11/14
Graphs1.
AbsDraw a line throughstee est art
Light exposure (mins)
2. From class averages
REMEMBER: n a
velocity ofe- flow
dec lamp dist
se pencThink about your units
AbsorbanceIn spectroscopy the absorbance A is defined as:
= -
I = transmitted light intensityintensity of light at a specified wavelength ( ) thathas passed through a sample
0 = nc en g n ens yintensity of the light before it enters the sample
EXPLAINED by the BEER-LAMBERT LAW
-
8/14/2019 101 Lab 6 photosynthesis Tutor template 2011.pdf
12/14
Determining an absorption spectrum
Alternative learningstyle
...http://nz.youtube.com/watch?v=_IV-E68rh18 ...
-
8/14/2019 101 Lab 6 photosynthesis Tutor template 2011.pdf
13/14
MASTER SLIDE
FINALCHECKLIST: Assignment - name; stream number;bench (#)
Clean Up instructions on
XXX xxxxxxxxxxxxxxxxxWash hands before leaving
-
8/14/2019 101 Lab 6 photosynthesis Tutor template 2011.pdf
14/14