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Welcome to Bio181L!

Welcome to Bio181L!

Contemplating the nuts & bolts of LifexVivo’s 3D cell animation

(also on TA desktop--Lab01TaoTA => Biovisions => BioVisionsPlayer.swf)

Contemplating the nuts & bolts of LifexVivo’s 3D cell animation

(also on TA desktop--Lab01TaoTA => Biovisions => BioVisionsPlayer.swf)

2About me...

• Kelsey Berg

• kberg@email.arizona.edu

• Majors in Physiology/Molecular and Cellular Biology, Minor in Chemistry

• From Phoenix, Arizona

3What 181L is

• Thinking, Understanding, Investigating, Evaluating

• Not arrive, assembly line, leave

• Not synchronized with most lecture sections, but internally coherent & mutually reinforcing

Science and this course

• “One of the beautiful things about science is that it allows us to bumble along, getting it wrong time after time, and feeling perfectly fine as long as we learn something each time.”

4

5About you

• NOTE: The section dance is now over

• Once a lab has met, no one can change into it

Last name, First name Section #

Year Major(s)Minor(s)

What do you want to do (career)?Where are you from?What do you expect from this course?

Anything else that I should know about you (optional)?

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About the manual• Reading it will prepare you for class & in-class quizzes

• Reading prior to 5’ before class will allow you to understand & think about what you’re reading

• Lays out Philosophy (vii), Learning Goals (ix), expectations (xi)

• Contains Periodic Table (0-1) and Molecular Basics (0-3), rules of molecule pics (0-5) and a cell (0-7)

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181 Lab ACCOUNTS• http://blc.arizona.edu/courses/181lab

• Click ‘Create Accounts’

•Your section number is: XX

• Bio181L_Go is the best way to do your work. Browser not so much

880% of success is just showing up*

• Absences must be excused from from Asya Roberts in BSE109 prior to making up

• Arriving late is absent. Leaving early is absent.

• Labs cannot be offered week after they are delivered; Avoid missing a lab

• Missing 2 labs blows a huge hole in the intellectual content. You may be dropped from the course--excused or otherwise

*--Woody Allen

9My Webpage• Get there via homepage* => Instructors => (Section #)

*http://blc.arizona.edu/courses/181Lab (Lab Man yellow page)

10Web page operation

11What to expect• Homework is serious--start early, and work in bursts, with

gaps. Generally, 2-3 components/week

• You will play a major role in experiment, protocol design

• i.e., you’ll need to know what you’re trying to achieve rather than showing up & following steps. Sometimes, there aren’t any

12Policies & Grading

• Syllabus (Linked on course homepage)

• Honor code & Plagiarism (Manual, p. xiii)

• Assignments

• On-line assessments/tutorials (10%)

• In-class quizzes (15%)

• Lab assignments (LABAs--Lab Activity Based Assignments) (50%)

• Lab Projects & reports (combine to 25%)

1313

the Tao of Moleculesthe Tao of Molecules

How molecules feel & the world they live in

How molecules feel & the world they live in

How? Why?

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Primary goalsPrimary goals

• Create understanding by observation, reasoning

• Chemical foundations for the course:• Water & its properties• non-watery things• Know molecules as real & tangible

things

• Create understanding by observation, reasoning

• Chemical foundations for the course:• Water & its properties• non-watery things• Know molecules as real & tangible

things

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From The Secret of Scent, Luca Turin p.28

When a journalist asked the great physicist Richard Feynman what single sentence would best encapsulate all science so far if it were to be the sole surviving scrap of all we knew, he replied, “The world is made of atoms.”

When a journalist asked the great physicist Richard Feynman what single sentence would best encapsulate all science so far if it were to be the sole surviving scrap of all we knew, he replied, “The world is made of atoms.”

16Atoms: They’re how life works

• DNA, RNA: C, H, N, O, P, [Mg++]

• Carbs: C, H, O

• Protein: C, H, N, O, (S), (P), [traces]

• Membranes: C, H, N, O, P, (S)

What cannot be done with assemblages of these atoms* cannot be done by living organisms, nor their

cells, nor their spit, etc.

*OK, fine, there’s the occasional role for Ca++, etc.

17Who am I?• At birth, # protons = # electrons

• Atoms seek completion, which means outermost electron set = 8 (hydrogen, helium it’s just 2)

Freeman Fig. 2.1a

18Coloring your world

19Four views of Water

H2O

HO

H

See lab manual, p. 0-3

(There will be a test)+

+

-

20Interacting with H2O

To your StructViewers!Desktop => Bio181L_Go

2121

Doing itDoing it

22Clean up!

• Oil waste in the hood

• ethanol waste in the sink

• anything too messy wadded up and discarded

23“Salting out”• It’s a real term; used to refer to a situation where the

addition of salt (portable charges) alters the solubility of other molecules in water

• Why should this be?

24Evaporation• First things first--what is it? What’s going on in terms of

molecules

• Experiment: Ethanol and water on your arm: which is cooler & why?

• Prediction: based on molecular weight, which should evaporate more quickly--H2O (2 x 1 + 1 x 16) or CH3CH2OH (5 x 1; 2 x 12; 1

x 16)

• If not, why not?

• Salt--ever tasted your sweat? Or anybody else’s for that matter?

• What benefit might there be to adding NaCl to water that you are intending to evaporate?

25Some advice

http://www.damnlol.com/please-do-me-right-now-224.html

26What’s an Assessor?

=>Chatting<=

27What’s vocabulary homework?

• EITHER both versions of the crossword exercise

• OR VocabuWary with a better than threshold score

• For “Atoms & Molecules”, F12, that’s 60,000

• Errors count off. Slowness counts off.

2828Homework due 10 p.m. before

lab

Homework due 10 p.m. before

lab

Assessor: 181 Intro ’12Vocab Atoms/Mols: xWord(x2) or ‘Wary

Assessor: Molecular World Tutorial

Assessor: 181 Intro ’12Vocab Atoms/Mols: xWord(x2) or ‘Wary

Assessor: Molecular World Tutorial

Next week’s quiz will includeConcepts from today

Atom colorsDeducing partial charges (from tutorial)

Manual Ch. 2

Extensive! In Depth!