1. laboratory safety, use of the facility and lab equipment training …€¦ · laboratory safety,...

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BSL-3 Core Facility Yong Loo Lin School of Medicine National University of Singapore

FO-005

BSL-3/ABSL-3 TRAINING AND CERTIFICATION PROGRAMME

Name of the trainee

Designation

Dept/Unit

Name of the PI

Tel no.

E-mail address

PART A. Training Checklist The following items shall be explained or demonstrated by the trainer

1. Laboratory safety, use of the facility and lab equipment training

(Module 1)

A minimum of hrs of training has been given in the following procedures:

S/N TOPIC CHECK BOX

COMMENTS/REMARKS

1 Overview of BSL-3 biorisk

management policy,

organization structure and BATA

requirements, WSH Act

including risk assessment and

management (BSL-3 Core

Facility Safety Manual, Chapter

1, 6.1)

2 Roles and responsibilities,

(BSL-3 Core Facility Safety

Manual Chapter 4)

3 BSL-3 health surveillance ,

security clearance, training,

proficiency testing and access

requirements, annual

requirements (BSL-3 Core

Facility Safety Manual, section

2

6.2, 6.3, 7.4.2.4)

4 General BSL-3/ABSL-3 rules

and practices (BSL-3 Core

Facility Safety Manual section

7.1 & 7.2)

5 Good microbiological techniques

including hazard communication

and handling blood and blood

products (BSL-3 Core Facility

Safety Manual section 6.5 and

7.14)

6 Use of laboratory equipment and

consumables (pass-through

cabinet between the corridor and

BSL-3 anteroom, autoclaves,

BSCs, centrifuges, incubators,

freezers, fridge/freezers, vacuum

pump, vortex, heat block,

pipettes)

Specify other equipment:

7 Familiarize with the layout of the

facility including emergency

escape routes.

8 Entry and exit procedures SOP-

CP-001(checking in/out, reading

room ventilation signals,

gowning/degowning PPE, PAPR

usage, use of pass-through

cabinets in changing/degowning

room)

9 Use of special equipment (cell

sorter, cell analyzer , )

Specify other equipment:

10 Facility maintenance

11 Equipment maintenance

Specify equipment:

The items on the above checklist have been explained and/or demonstrated to me.

Date : Trainee’s signature

Date : Trainer’s name and signature

3

2. Waste management and transporting/storing/handling of pathogens and chemicals (Module 2)


S/N TOPIC CHECK BOX

COMMENTS/REMARKS

1 Biowaste management in the

laboratory (BSL-3 Core Facility

Safety Manual section 7.12)

2 Storage of biological agents and

materials within the laboratory


Manual section 7.9)

3 Importing /exporting and

transporting biological agents

and materials (BSL-3 Core


7.6)

4 Handling and storing of

chemicals/radioisotopes

(Chemical and Radioactivity

SOPs)

5 Handling of liquid cultures and

agar plates (BSL-3 Core Facility

Safety Manual 7.14.2 & 7.14.3)

6 Preparation and use of

disinfectants (Chemical SOPs)

7 Handling and disposing sharps


Manual section 7.12.1.4)

8 Housekeeping (keeping

consumable, cleaning routine,

laundry, refilling supplies) BSL-3

Core Facility Safety Manual

section 7.13



Date : Trainer’s name and signature

4

3. Emergency response training (Module 3)


S/

N TOPIC

CHECK BOX COMMENTS/REMARKS

1 Alarms and responses/conducts

2 Ventilation failure (BSL-3 Core


7.15.8)

3 Fire (BSL-3 Core Facility Safety

Manual section 7.15.7)

4 Power failure (BSL-3 Core


7.15.9)

5 Conduct during sudden stoppage

of biosafety cabinet (BSL-3 Core


7.15.1.7)

6 Conducts during infectious and

non-infectious medical

emergency and personnel

pathogen exposure (BSL-3 Core


7.15.1 and 7.15.4)

7 Needles/sharp injury response


Manual section 7.15.1.4)

8 Burn injury response (BSL-3


section 7.15.2)

9 Spill response conduct (BSL-3


section 7.15.1)

10 Accident/Incident reporting

Anonymous reporting (BSL-3


section 6.7)

11 Location and use of first aid kits,

AED, fire extinguishers, manual

call points, fire blanket, hose reel,

exits (BSL-3 Core Facility Safety

Manual Appendix)

12 Contact information about

Emergency Response Team and

5

its responsibilities (BSL-3 Core

Facility Safety Manual Chapter 2)

14 Security breaches (SOP-ER-004)



Date: Trainer’s name and signature

6

4. Experiment/protocol based training (Module 4)

S/N TOPIC

CHECK BOX

COMMENTS/REMARKS

1 Route of transmission, signs and symptoms of

infection and disease caused by respective

pathogen

2 Preventive measures and post-exposure

evaluation and follow-up plan

3 User-specific experimental protocols

(depending on the type of experiments to be

carried out, e.g. microbiology, virology,

animal experiments)

Date: Trainee’s signature

Date: PI’s name and signature

A minimum of hrs training has been given in the following protocols:

S/N Name of Protocols

Certificatio

n Date

Trainee’s

Signature

Trainer’s

signature

First

Examiner

Signature

Second

Examiner

Signature

1

2

3

4

5

6

7

8

9

10

7

11

12

13

14

15

16

17

18

19

20

21

22

23

24

25

26

27

28

29

30

31

8

5. Animal related training (Module 5)


S/N TOPIC CHECK BOX

COMMENTS/REMARKS

1 Knowledge of IVC cage system

(SOP-ABSL3-012 and 013)

2 Cage changing (SOP-ABSL-3-

001)

3 Routine rodent husbandry tasks

(SOP-ABSL3-005)

4 Weekend monitoring of animals


Manual section 7.3.2)

5 Lighting control system and

alarms in the animal rooms

6 Infecting / dissecting animals and

homogenizing tissues

7 Handling of animals in a

biosafety cabinet

8 Handling of needles and syringes

for animal experiments (BSL-3


7.11.1)

9 Disposal of animal carcasses/

wastes (SOP-ABSL3-004)

10 Transporting of infected animal

cages from the experimental

room to autoclaves (SOP-

ABSL3-001)

11 Response to an infected animal

escapes from the biosafety

cabinet and to animal bite/scratch.


Manual 7.15.1.6)

12 Transporting of autoclaved

infected animal cages from the

BSL-3 to MD2. (SOP-ABSL3-

007)

The protocols on the above checklist have been explained and/or demonstrated to me.

9

Date: Trainee’s signature:

Date: Trainer’s name and signature:

6. Compulsory reading of following relevant policy, SOPs and protocols

S/N Titles CHECK

BOX

Comments/Remarks

1 BSL-3 safety policy (BSL-3 Core


1.2)

2 Standard Entry and Exit (SOP-

CP-001)

3 Waste management (BSL-3 Core


7.12)

4 Emergency procedures (BSL-3


section 7.15)

5 Reporting of Incident/Accident


Manual section 6.7)

6 Procurement, storage and

transport of infectious samples


Manual section 7.6)

7 Operational control (BSL-3 Core

Facility Safety Manual chapter 7)

8 Biosecurity and Noncompliance


Manual sections 6.4 and 6.8)

9 Risk assessments and

management (BSL-3 Core

10


6.1)

I have read the above BSL-3 policy, SOPs, protocols and their risk assessments. I understood contents of these documents. I certify that I will adhere to the procedures as described in the documents.

Date: Trainee’s signature

11

BSL-3 Biosafety Committee use only

All training completed Yes No Date:

Name and signature of supervisor/PI:

Date:

Name and signature of Head of BSL-3 Operations:

Date:

Name and signature of BSL-3 Biosafety Committee Chairperson:

Date:

12

PART B: CERTIFICATION EXAM RESULTS

Module 1: Passed : Yes No

Comments:

Name/ Signature of the first examiner__________________________________

Date:

Name/ Signature of the second examiner__________________________________

Date:


Comments:


Date:


Date:


Comments:


Date:


Date:


Comments: See Section Module 4 for the protocols certified to work

13


Comments:


Date:


Date:

14

PART C. ACKNOWLEDGMENT

This will certify that I have been informed of the potential hazards posed by the research to

be conducted in the YLL SoM BSL3 laboratory. I have undergone a period of required training

and an appropriate medical evaluation prior to working in the YLL SoM BSL3 facility. I am

familiar with the safety policy, guidelines and regulation standards and emergency procedures

described in the BSL-3 Core Facility Safety Manual. I will report immediately any known or

suspected exposure to the agent or symptoms of infection with the agent to the biosafety

coordinator and my PI.

Date:

TRAINEE’S SIGNATURE

BSL-3 Facility Operator use only

BSL-3 Access granted Yes No

Date:

Name and signature of Head of BSL-3 Operations:

Date:

Name and signature of BSL-3 Core Facility Director:

Date:

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