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INTERNATIONAL SEED TESTING ASSOCIATION (ISTA) www.seedtest.org

Bulking and Sampling CommitteeBulking and Sampling Committee

Report 2008‐2009Report 2008 2009


B lki d S li C ittBulking and Sampling CommitteeChair: Leena Pietilä FinlandChair: Leena Pietilä FinlandVice‐chair: Max Soepboer The NetherlandsMembers: Helena Blomqvist‐Ljung Sweden

lLaura Carlson USALotta Claesson SwedenDidier Demilly FranceEddie Goldschagg South AfricaFabio Gorian ItalyGerry Hall United Kingdomy gTine Vilbrad Jørgensen DenmarkMichael Kruse GermanyAlfred Odindo KenyaAlfred Odindo KenyaDot Vittrup Pedersen DenmarkMable M. Simwanza Zambia


Contents• Rules development

• Method validationMethod validation

• Ongoing projects

• Workshop

• 2009‐2010 perspectives• 2009‐2010 perspectives


Proposal to decrease submitted sample size for Nicotianasample size for Nicotiana

• Submitted sample size for Nicotiana tabacum is 25 g • Proposed to be decreased to 5 g

– 1000 seed weight approx. 0.08 gg pp g– working sample size for purity analysis 0.5 g (Table 2A, Part 1)( , )

– working sample size for count of other species 5 g (Table 2A, Part 1)( , )

– ISTA Rules I‐2.2: submitted sample size should be ten times larger than the working sample size for g g ppurity


Proposal to include cargo sampler and vertical use of spiral trier to the Rules

• Prof. Dr. Michael Kruse will present the poutcome of the validation project


Trier validation

• Validation will be continued with tube trierValidation will be continued with tube trier

• Cargo sampler for big seeds will be proposed b dd d h R lto be added to the Rules

• Also other types of triers may be validated if yp yinterest

• How the validation is done needs thorough• How the validation is done needs thorough review


Sampling for seed mixture experiment

• Participating ISTA laboratories not expected to• Participating ISTA laboratories not expected to sample only for the experiment, but e.g. control samples drawn for other purposes can be usedsamples drawn for other purposes can be used

• Sampling is carried out according to the ISTA Rules, Ch 2Ch. 2

• Maximum lot size: components grouped according to max. lot size. Max. lot size of the mixture = lot size of most abundant species group


ISTA/ISF experiment on herbage seed lot size

• Three warehouses from different companies• Three warehouses from different companies have fulfilled requirements: – at the minimum 5 homogeneous seed lots out of 6 tested

– Quality manuals approved by national designated ISTA member laboratoriesg

• Four other warehouses participating


… ISTA/ISF experiment on herbage d l iseed lot size

• Continuing at latest till 2013• Continuing at latest till 2013

• Difficult to form an opinion due to lack of data

• BSC will ask data concerning control samples• BSC will ask data concerning control samples from the national authorities


Workshops

• A workshop for sampling was intended to• A workshop for sampling was intended to arrange in Zambia in April 2009

• The workshop was cancelled due to lack of participantsparticipants

• A training course for trainers is discussed in h BSCthe BSC


Questions to the Committee• Sampling equipment: dividers, triers, automatic seed samplersp

• How to take samples from different kind of containerscontainers

• ISTA/ISF Herbage Seed Lot Size Experiment


Future perspectivesISTA/ISF i h b d l i• ISTA/ISF experiment on herbage seed lot size– continues

d• Seed mixture experiment – BSC will analyse the data

i ill h d f– experiment will run up to the end of 2009

• Trier validation – Continues with new types of triers

• Requirements for Nobbe trier are revised• Handbook on Seed Sampling

– revision continues


Thanks toThanks to

Members of the BSCMembers of the BSC

ISTA Secretariat

Ronald Don

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