1 april, 2005 chapter c4.1 and c5.1 dna microarrays and cancer
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1 April, 2005
Chapter C4.1 and C5.1
DNA Microarrays and Cancer
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Overview• DNA microarrays allow the researcher to assay the
transcription of a number of genes simultaneously.
• This technique is especially informative regarding large-scale changes in gene expression.
• Global analysis of gene expression is of particular interest in the understanding of cancer - a disease of gene expression.
• Cancer cell type and progression status can be probed using mmicroarray methods.
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Differentially labeled
cDNA sets:
green = control,
red = experimental
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Hybridization
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Microarray Data
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Microarray and Northern Data
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Clustering of Microarray Data
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Global Shift in Gene Expression
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Global Shift in Gene Expression
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DLBCL Microarray Experiment
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Clustering in the DLBCL Experiment
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Discovery of DLBCL Subtypes
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Clinical DLBCL Outcomes
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Expression Changes in
Breast Cancer
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Cluster Analysis
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Microarray Detection of Genomic Rearrangements
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Southern Confirmation
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