08 su hinh thanh mot dap ung mien dich

Download 08 Su Hinh Thanh Mot Dap Ung Mien Dich

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  • 1S HNH THANH MOT

    AP NG MIEN DCH

    GS Pham Hoang Phiet

    BS Huynh Thanh Bnh

  • 2. .

    ong hoc mot ap ng mien dch

  • 3So sanh ap ng th 1 va th 2

    MD

    Tnh chat

    Th 1 Th 2

    Dien

    tien

    Tiem an 1-2 tuan 3-5 ngay

    Tang SLKT Tang nhanh Tang rat nhanh

    Bnh on 2-3 tuan Nhieu thang- nam

    Giam sut Nhanh T t

    So

    lngCao hn th 1 nhieu

    Chat

    lng

    Lp KT IgM IgG

    Ai lc vi

    KN+ +++

  • 4Te bao trnh dien

    khang nguyen

    HLA lp II TCD4(peptid 15-34 aa)

    HLA lp I TCD8(peptid 9 aa)

  • 5Class I MHC Pathway

    Viral protein is made

    on cytoplasmic

    ribosomes

    Plasma membrane

    Proteasome

    degrades

    protein to

    peptides

    Peptide transporter

    protein moves

    peptide into ER

    MHC class I alpha

    and beta proteins

    are made on the rER

    Peptide associates

    with MHC-I complex

    Peptide with MHC

    goes to Golgi body

    Peptide passes

    with MHC from Golgi

    body to surface

    Peptide is presented

    by MHC-I to CD8

    cytotoxic T cell

    Golgi body

    rER

    Globular viral

    protein - intact

  • 6Class II MHC Pathway

    Globular

    protein

    Endocytosis

    Protein is processed to

    peptides in endosome

    or lysosome

    Endosome

    Lysosome

    Fusion of endosome

    and exocytic vesicle

    Endoplasmic reticulum

    Class II MHC

    Synthesis

    3 chains: , and Ii

    Golgi

    body

    Exocytic vesicle fuses

    with endosome

    releasing Ii from dimer

    Ii

    Immunodominant

    peptide binds

    to class II MHC

    Endosome fuses with

    plasma membrane

    Peptide MHC-II

    complex is presented

    to CD4 helper T cellCD4 helper T cell

  • 7IL2 (IL4)

    TB,Tc, (B)

    Hoat hoa Lympho T

    1

    23

    4

  • 8Points Concerning Antigen

    Processing and Presentation1. Location of pathogen

    viruses in cytosol, MHC class I pathway, Tc response

    extracellular bacteria, MHC class II pathway, Th2 response, Ab formation

    intracellular bacteria, MHC class II pathway, Th1 response

  • 9Points Concerning Antigen

    Processing and Presentation2. Peptides derived from both self and

    non-self proteins can associate with

    MHC class I and class II molecules.

    3. Chemical nature of MHC groove

    determines which peptides it will

    bind.

  • 10

    Hoat hoa Lympho bao

    T B

    Tn hieu

    KN

    (1)Ti (TCR) KN

    (2) CD4 (TH)

    HLAII(APC)

    sIg (BCR) KN

    Tn hieu

    th 2

    (1) IFN- (TH)

    (2) IL1 (APC)

    IL2,IL4, IL5

  • 11

    Hoat hoa

    Lympho B

  • 12

    S hp tac gia

    ai thc bao,

    lympho T va

    lympho B

  • 13

    Cellular Events in 1o Response to

    T-dependent Ags

    Lag

    Clonal selection

    Log

    IgM

    Class switching

    Stationary

    Decline

    Memory Cell Pool

    IgM

    Memory Cells

    IgG

    1o Ag

  • 14

    Cellular Events in 2o Response to

    T-dependent Ags Lag phase

    Virgin cells

    Memory cells

    Log phase

    Pool size

    IgG, IgA or IgE

    Stationary

    Decline

    Sustained production

    IgM

    Memory

    Cells

    IgG

    IgG

    Memory

    Cells

    Memory

    Pool

    Virgin B cell

  • 15

    Hieu ng Hapten-tai

    Hapten khong co tnh gay MD

    Hapten phai gan vi 1 protein tai => phc hapten tai (hapten-carrier

    complex)

    Th nhan dien tai, B nhan dien hapten

    Tai th 1 va th 2 phai giong nhau.

  • 16

    S hp tac han che trong nhom phu hp mo

    (hien tng Zinkernagel va Doherty)

    Mouse

    Strain A or BImmunize

    with antigen

    Period of 7 days

    T cells isolated

    from lymph nodes

    T cell primed with

    antigen

    Mouse

    Strain A or B

    Peritoneal macrophage

    Antigen

    Macrophage presents antigen

    Macrophages and

    T cells are co-cultivated

    Anti X-induced

    Macrophage T-cell proliferation

    Strain A A yes

    Strain A B no

    Strain B B yes

    Strain B A no

    Assay DNA

    synthesis

    after 48 hr

  • 17

    Tc Cell Self MHC RestrictionMouse

    Strain A or B

    Immunize

    with virus

    Period of 7 days

    Virus-specific

    cytotoxic T cells (CTLs)

    isolated from spleen

    Virus-specific

    CTLs

    Mouse

    Strain A or B

    Purify

    Fibroblasts

    CTLs and fibroblasts

    are co-cultivated

    Spleen Fibroblast 51Cr release

    CTLs (target cells) from fibroblasts

    immunized infected (lysis)

    with with

    Strain A A yes

    Strain A B no

    Strain B B yes

    Strain B A no

    Assay 51Cr

    release

    Isolate spleen

    Infect fibroblasts

    with virus and

    radiolabel with 51Cr51Cr labeled fibroblast

    presents antigen

  • 18

    Self MHC Restriction in the Thymus4 -

    8 low

    4 low

    8 low

    4 + 8 +

    TCR

    4 + 8 +

    TCR

    macrophage

    Productive TCR

    rearrangementNon-productive TCR

    rearrangement

    Recognise self MHC

    Not recognise self MHC

    TCR does not

    recognise self antigens

    Negative selection

    TCR recognises

    self antigens

    APOPTOSIS

    4 + 8 +

    TCR 4 - 8 -

    Sub-capsular region

    Cortex

    Cortico-medullary region

    4 + 8 -

    TCR 4 - 8 +

    TCRvessel

    Medulla

  • 19

    KN khong le thuoc thymus

    Cau truc: ai phan t do trung phan cac n v nho (lipopolysaccharide, dextran)

    Cac epitop giong nhau tren ai phan t lien ket vi nhieu sIg tao thanh am (hien tng

    oi mu) => hoat hoa Lympho B thanh tng

    bao => SX KT

    KT thuoc lp IgM, khong tao ra tr nh MD

  • 20

    Hien tng oi mu