07a3bs06 applied chemistry and biochemistry

7/27/2019 07a3bs06 Applied Chemistry and Biochemistry

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Code No: 07A3BS06 Set No. 1

8. (a) Write the general methods of biochemical analysis of glucose present in theblood.

(b) Name the different types of chromatographic techniques and explain the useof UV lamp for spotting in TLC. [10+6]

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II B.Tech I Semester Regular Examinations, November 2008APPLIED CHEMISTRY AND BIOCHEMISTRY

(Bio-Medical Engineering)Time: 3 hours Max Marks: 80

Answer any FIVE QuestionsAll Questions carry equal marks

1. (a) How do you determine the pH of a solution using a reference electrode?

(b) What is single electrode potential? How do you determine the emf of a galvaniccell? [8+8]

2. (a) What is polymerization?

(b) Describe the process of co-polymerization with an example.

(c) Differentiate thermoplastics from thermosetting plastics? [4+8+4]

3. (a) What are the various additives used to induce or improve the essential prop-erties of lubricating oils.

(b) Distinguish between fluid film and boundary lubrication.

(c) Define viscosity and viscosity index? What is its significance? [6+4+6]

4. (a) What are cell organelle?

(b) Describe each organelle and its function.

(c) What are the differences between plant and animal eukaryotic cells? [2+8+6]

5. (a) Define specificity of an enzyme and explain different types of specificities ex-hibited by enzymes.

(b) Differentiate between the following terms:-

i. Competitive and non-competitive inhibition

ii. Coenzyme and isoenzymes. [8+8]

6. (a) Write a note on the structure of steroids.

(b) Explain the functions of cholesterol. [8+8]

7. (a) What are erythrocytes? Explain their origin, structure and functions.

(b) Describe the structure and functions of a hemoglobin. [8+8]

8. (a) Explain various detectors and burners involved in flame photometry.

(b) What is the significance of excess presence of glucose in blood? [10+6]

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1. (a) Outline the experimental setup and procedure to determine the specific con-ductivity of 0.1N Solution of KCl at room temperature.

(b) What is cell constant? What is its unit? How do you determine it? [8+8]

2. (a) Give the monomers of the following:

i. PVC

ii. HDPE

iii. Polythene

(b) Describe the preparation and engineering uses of Bakelite? [8+8]

3. Suggest suitable lubricants for each of the following systems with justification:

(a) Internal Combustion Engines

(b) Cutting tools

(c) Gears

(d) Steam turbines. [16]

4. Write short notes on:

(a) Electron transport complexes

(b) Respiratory inhibitors

(c) Golgi complex or golgi apparatus

(d) Cytochromes. [16]

5. (a) Describe the reversible and irreversible mode of inhibition of enzymes withsuitable examples.

(b) Explain the laws of the Spectrophotometry and how these are useful in thestudy of enzymes. [6+10]

6. (a) Describe the replication of DNA.

(b) Write short notes on:

i. Replication fork

ii. RNA polymerase

iii. Reverse Transcription. [7+9]

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7. (a) What is electrophoresis? How it is useful? Write a note on zone electrophore-sis.

(b) What is meant by salting out of proteins? Explain the separation of bloodserum proteins by electrophoresis. [8+8]

8. (a) What is normal and reversed phase partition chromatography?

(b) Write briefly on the separation of amino acids by one and two dimensionalchromatography. [8+8]

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1. (a) Write down the Nernsts equation and explain the various terms involved?

(b) What are its important applications?

(c) Explain the source of current in a galvanic cell with an example and the

necessary equations. [6+4+6]

2. (a) How is rubber isolated from latex?

(b) Describe the preparation and engineering uses of Bakelite?

(c) Write a note on vulcanization of rubber? [6+8+2]

3. (a) Describe hot-limesoda process of water treatment and what are its advantagesover cold process?

(b) Calculate lime and soda required for treating 10,000 litres of a sample ofwater containibg the following in milligrams per litre.Calcium Carbonate 8.1,

Magnesium Sulphate 12, calcium sulphate: 13.6,Sodium Chloride 4.6. [10+6]4. (a) What is Golgi complex?

(b) Describe the lipid bilayer arrangement for plasma membrane.

(c) List the three major structural elements of Cytoskeleton and give the generalfunction of each. [4+8+4]

5. (a) What are plasma functional enzymes? Explain.

(b) How will you carryout diagnosis of Wilsons disease? [6+10]

6. (a) Write about the preparation and characterization of reagents used in Radio

Immuno Assay.(b) Differentiate between nucleotides, nucleosides & organic bases present in nu-

cleic acids. [8+8]

7. (a) What do you understand by blood sugar levels in the body and what is itsclinical significance?

(b) Explain one best method for estimation of glucose in a blood sample. [8+8]

8. (a) Name the important techniques used for the measurement of activity of radioisotopes.

(b) Describe the important applications of isotopes. [8+8]

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07a3bs06 applied chemistry and biochemistry

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