Uptakes of SPIO by Murine Macrophages and Monocytes

Daniel ChanUniversity of Colorado Health Sciences Center

12-19-2001

Synthesis of SPIO

• 1. SPIO coated with chondroitin sulfate• 2 SPIO coated with standard dextran

(6K)• 3 SPIO coated with Amino-silane• 4 SPIO coated with lipids• 5 Lipid encapsulated SPIO coated with

amino-silane

Uptakes of SPIOs by murine peritoneal macrophages and monocytes

• Normal mice were treated with mineral oil by ip injection.

• 24 hours later, equal amount of various SPIOs were injected intraperitoneally.

• 24 hours later, peritoneal macrophages and monocytes were isolated.

• Uptakes of various SPIOs were examined by light microscopy.

Uptake by Mouse Macrophages

SPIO coated with chondroitin sulfate

Uptake by Mouse Macrophages

SPIO coated with standard Dextran (6K)

Uptake by Mouse Macrophages

SPIO coated with amino-silane

Uptake by Mouse Macrophages

SPIO coated with lipids

Uptake by Mouse Macrophages

Lipid encapsulated SPIO coated with amino-silane

Uptake by Mouse macrophages

SPIO coated with CS

Uptake by Mouse Macrophages

SPIO coated with Dextran (6k)

Uptake by Mouse Macrophages

SPIO coated with Amino-silane

Uptake by Mouse Macrophages

SPIO coated with lipids

Uptake by Mouse Macrophages

Lipid encapsulated SPIO coated with amino-silane