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0517-6611(2018)21-0001-04
SeparationofMarineActinomycetesandScreeningofAntagonisticBacteriaTIANYunfang,FUZeqin,YEYingying (NationalEngineeringResearchCenterforMarineAquaculture,MarineScienceandTechnologyColleage,ZhejiangOceanUniversity,Zhoushan,Zhejiang316022)Abstract [Objective]SymbioticorepiphyticactinomyceteswithantibacterialactivesubstanceswereisolatedandscreenedfromcommonintertidalzonemarineanimalsinZhoushan.Differentmethodswereusedfordeterminingtheantimicrobialactivityandantimicrobialspectrum,theclassificationstatusofantagonisticbacteriawasalsoidentifiedforprovidingbasicdataforstudyingthesourceofmarineantibacterialsubstances.[Method]Throughseparationandpurificationtechnology,strainswereobtainedfromseveralintertidalzonemarineanimalsinZhoushan.StrainswithcertainbacteriostaticeffectsonStaphylococcusaureuswereobtainedafterinitialscreeningbyspotinoculation.Antimicrobialactivitywasquantitativelydeterminedbyenzymeassay.Thehighefficiencyantagonisticbacteriastrainswereobtainedbyfilterpaperdiffusionmethod.Escherichiacoli,MoniliaalbicanandBacillussubtiliswereusedasindicatorstodeterminetheirantimicrobialspectrum.[Result]ThetypicalstrainsGP57andGT16wereidentifiedbyphysiological,morphologicaland16SrDNAmolecularidentification.AllofthemwereStreptomyceswithasimilarityof99%and100%respectively,GT16wasidentifiedasaStreptomycesgriseoplanus.[Conclution]Amongmarinesymbioticorepiphyticmicrobes,marineactinomycetesmaybeaverygoodsourceofbacteriaproducingantibacterialactivesanddeservefurtherstudy.Keywords Intertidalzone;Marineanimals;Symbioticorepiphyticmicrobe;Antimicrobialactivity;Screening
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)
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[1]!"#
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[J]../01
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[2]78
.9:3$%;3<2(=><?)*+
[J].@AB*+
,2008(2):227-230.
[3]HOLMSTR?MC,KJELLEBERGS.MarinePseudoalteromonasspeciesareassociatedwithhigherorganismsandproducebiologicallyactiveextracellularagents[J].FEMSMicrobiologyEcology,1999,30(4):285-293.
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,2015,34(4):541-552.[4]RADZKIW,ZIAJASOTYSM,NOWAKJ,etal.EffectofprocessingonthecontentandbiologicalactivityofpolysaccharidesfromPleurotusostreatusmushroom[J].LWTFoodScienceandTechnology,2016,66:27-33.
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,2015(21):152-154.[8]OOIVEC,LIUF.Areviewofpharmacologicalactivitiesofmushroompolysaccharides[J].Internationaljournalofmedicinalmushrooms,1999,1(3):195-206.
[9]ZHANGJJ,MENGGY,ZHAIGY,etal.Extraction,characterizationandantioxidantactivityofpolysaccharidesofspentmushroomcompostofGanodermalucidum[J].Internationaljournalofbiologicalmacromolecules,2016,82:432-439.
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