0.1

17*

2126

78

96

10*11*945*

100

99

72

91

6

27

69

13

16

18

19

9894

83

14

1215

85

78

25 28*

85

23*

20*22*

88

79

62

24

2

371

1*53

1. CAD13539 Ralstonia solanacearum AcrA2. YP_585729 Ralstonia metallidurans AcrA3. AAZ62798 Ralstonia eutropha AcrA4. YP_406903 Shigella flexneri5. NP_289833 E. coli O157:H76. ABB31051 Geobacter sulfurreducens7. YP_621022 Burkholderia cenocepacia8. CAH34805 Burkholderia pseudomallei9. NP_459471 Salmonella typhimurium10. AAQ21215 Erwinia amylovora11. BAC77250 Serratia marcescens SdeX12. ABA74634 Pseudomonas fluorescens Pf0-113. NP_768155 Bradyrhizobium japonicum14. CAC41580 Mesorhizobium loti

Supplementary Figure 1

15. AAY37326 Pseudomonas syringae B728a16. AAO56097 Pseudomonas syringae DC300017. P43505 Neisseria gonorrhea MtrC18. CAJ24703 Xanthomonas campestris19. AAM37349 Xanthomonas campestris pv. citri20. AAO49780 Pseudomonas putida SepA21. EAO12292 Xylella fastidiosa22. AAK69563 Pseudomonas putida TgtT23. AAD15663 Pseudomonas stutzeri TbtA24. AAL44019 Agrobacterium tumefaciens c5825. AAO57754 Pseudomonas syringae DC300026. AAM35219 Xanthomonas axonopodus pv. citri27. CAD17970 Ralstonia solanacearum Rsp081928. AAG03814 Pseudomonas aeruginosa PAO1 MexA

0.1

4*2122

97

94

1430

89

2425

88

3235

84

11

28

31

33 74 56

56

53

1229

672734*

64

88

23

2610

6953

83

52

8*

9*

99

20 5*16*

100

6* 7*

100

99

17 8

83

71

90

93

13*

19

2*

15*

92

3

1

77

73

1. CAD17315 Ralstonia solanacearum “matE” Rsp0164

2. BAB68204 Vibrio parahaemolyticus “VmrA” NorM family

3. CAD18364 Ralstonia solanacearum NorM family RSp1213

4. NP_521844 Ralstonia solanacearum DinF

5. NP_288099 E. coli MATE family NorA/YdhE

6. BAC59742 Vibrio parahaemolyticus NorM

7. BAB79260 Vibrio cholerae non-01 VcmA*

8. NP_188997 Arabidopsis thaliana AT3G23550 MDR

9. NP_191462 Arabidopsis thaliana AT3G59030 MDR

10. NP_418468 E. coli K12 dinF

11. AAL46115 Agrobacterium tumefaciens strain C58 dinF

12. AAO58273 Pseudomonas syringae DC3000 dinF

13. AAL00559 Streptococcus pneumoniae dinF

14. NP_767781 Bradyrhizobium japonicum USDA110 dinF

15. BAB70470 Vibrio cholerae VcrM*

DinF Family

Supplementary Figure 2

16. AAQ21212 Erwinia amylovora NorM*

17. AAF85483 Xylella fastidiosa 9a5c norM

18. Q879Z5 Xylella fastidiosa Temecula1 norM

19. AAO53914 Pseudomonas syringae DC3000 MATE family

20. CAD14882 Ralstonia solanacearum GMI1000 RSc1180

21. ZP_00460454 Burkholderia cenocepacia MatE

22. CAH35378 Burkholderia pseudomallei MatE

23. AAF93268 Vibrio cholerae el tor DinF

24. ZP_00631278 Paracoccus denitrificans MatE

25. ABA78359 Rhodobacter sphaeroides MATE family protein

26. RSY03136 Salmonella typhimurium LT2 Na+ driven MDR

27. RAV01352 Azotobacter vinelandii Na+ MDR

28. RMLO04680 Mesorhizobium loti MAFF303099 Na+ driven MDR

29. RPFL00460 Pseudomonas fluorescens Na+ driven MDR

30. RRPA02598 Rhodopseudomonas palustris Na+ driven MDR

31. RSM04712 Sinorhizobium meliloti Na+ driven MDR

32. RBX03146 Bacteroides fragilis Na+ driven MDR

33. RRLE05610 Rhizobium leguminosarum Na+ driven MDR

34. RPA06399 Pseudomonas aeruginosa PAO1 PmpM

35. RHD05071 Bacillus halodurans Na+ driven MDR