- Unit VI -- Unit VI -IAS Specimen Collection, IAS Specimen Collection,

Identification, and Identification, and PreservationPreservation

Rebecca M. Rebecca M. Westbrooks Westbrooks

Randy G. WestbrooksRandy G. WestbrooksSteven ManningSteven Manning

Global Invasive Species Global Invasive Species ProgrammeProgramme

Cape Town, South AfricaCape Town, South Africa

GISP Prevention Training GISP Prevention Training Course.Course.

Day Five – 15 December, 2006.Day Five – 15 December, 2006. 0900-0915. 0900-0915. Field Trip Evaluation.Field Trip Evaluation. (Sandy (Sandy Johnson).Johnson).

0915-0945. 0915-0945. Unit 6 - IAS Specimen Collection Unit 6 - IAS Specimen Collection and Identification.and Identification. (Becky Westbrooks). (Becky Westbrooks).

0945-1015. 0945-1015. Unit 7 – IAS Decisionmaking Tools, Unit 7 – IAS Decisionmaking Tools, IAS Risk AssessmentIAS Risk Assessment (Randy Westbrooks).(Randy Westbrooks).

1015-1030. 1015-1030. Tea.Tea. 1030-1100. 1030-1100. Unit 7 Lab Exercise - Unit 7 Lab Exercise - Weed Risk Weed Risk

Assessment – Beach Vitex.Assessment – Beach Vitex. 1100-11301100-1130. . Basic Elements of a National IAS Basic Elements of a National IAS

Prevention Strategy.Prevention Strategy. (Steve Manning). (Steve Manning). 1130-1200.1130-1200. The Way Forward -The Way Forward - Workshop Workshop

Summary and Next Steps for IAS Collaboration and Summary and Next Steps for IAS Collaboration and Partnering in Eastern, Central, and Southern Africa. Partnering in Eastern, Central, and Southern Africa. (Lynn Jackson, Becky Westbrooks, Steve Manning, (Lynn Jackson, Becky Westbrooks, Steve Manning, Randy Westbrooks).Randy Westbrooks).

1200-1230.1200-1230. Goodbyes, Pictures, Contact Goodbyes, Pictures, Contact Directory.Directory.

Specimen Collection and Specimen Collection and IDID

6.1. Specimen Collection 6.1. Specimen Collection GuidelinesGuidelines Wild Populations – Minimal # of Wild Populations – Minimal # of

SpecimensSpecimens Port Collections – Adequate # Port Collections – Adequate #

Specimens for IdentificationSpecimens for Identification 6.2. Specimen ID Resources6.2. Specimen ID Resources

Numerous Online Resources (Textbook Numerous Online Resources (Textbook P. 170)P. 170)

6.3. Traditional Dichotomous 6.3. Traditional Dichotomous KeysKeys

Series of Successive Couplets Series of Successive Couplets Couplet – Describes some Feature Couplet – Describes some Feature of Organism being Identifiedof Organism being Identified

Couplet 1. Seeds RoundCouplet 1. Seeds Round SoybeanSoybean Seeds OblongSeeds Oblong Go to #2Go to #2

Couplet 2. Seeds WhiteCouplet 2. Seeds White Navy Navy BeansBeans

Seeds Black Cuban Black Seeds Black Cuban Black BeansBeans

6.4. Computer Assisted ID – Online 6.4. Computer Assisted ID – Online ResourcesResources

Arbor Day FoundationArbor Day Foundation Tree ID KeyTree ID Key

Amateur Entomology Amateur Entomology Key to Insect IDKey to Insect ID

Earth Life Web Earth Life Web ProductionProduction Online Key to Insect Online Key to Insect

OrdersOrders Lucid Online ID KeysLucid Online ID Keys

Numerous Online Keys for Numerous Online Keys for Insects, Plants, and etc.Insects, Plants, and etc.

Lucid Key for U.S. Lucid Key for U.S. Federal Noxious Weed Federal Noxious Weed Seeds (USDA APHIS)Seeds (USDA APHIS)

Lucid Key for Scale Lucid Key for Scale Insects (USDA APHIS)Insects (USDA APHIS)

6.5. Online Invasive Species 6.5. Online Invasive Species Databases.Databases.

Global Invasive Species Network Global Invasive Species Network Coordinated by USGS BRDCoordinated by USGS BRD

Global Invasive Species Database Global Invasive Species Database World Conservation Unit – Invasive World Conservation Unit – Invasive

Species Specialist Group – Based in Species Specialist Group – Based in New ZealandNew Zealand

Plants Database Plants Database USDA Natural Resources USDA Natural Resources

Conservation ServiceConservation Service

6.6. DNA Bar Coding – 6.6. DNA Bar Coding – Consortium for the Bar Code of Consortium for the Bar Code of

Life (CBOL)Life (CBOL)

Practical Methods for Practical Methods for Rapid ID of the Rapid ID of the estimated 10 Million estimated 10 Million Species of Life of EarthSpecies of Life of Earth

Global Standard in Global Standard in Taxonomy – Especially Taxonomy – Especially for Non-Taxonomistsfor Non-Taxonomists

130 Member 130 Member Organizations in 40+ Organizations in 40+ Countries (Botanical Countries (Botanical Gardens, Zoos, Herbaria, Gardens, Zoos, Herbaria, etc.)etc.)

Louisiana Louisiana CrayfishCrayfish((Procambarus Procambarus clarkiiclarkii))

Principles of DNA Bar Principles of DNA Bar CodingCoding

Short Gene Short Gene Sequence from Sequence from Standardized Standardized Region of the Region of the GenomeGenome

DNA Bar Coding – DNA Bar Coding – Outgrowth of Outgrowth of Molecular BiologyMolecular Biology

6.7. Port ID Reference 6.7. Port ID Reference CollectionCollection

Specimen Specimen CollectionCollection Wild PopulationsWild Populations Specimens Specimens

Collected in the Collected in the PortPort

Specimen Specimen PreparationPreparation Insects Insects PlantsPlants SeedsSeeds

Plant DiseasesPlant Diseases PhotographsPhotographs

Aquatic Nuisance Aquatic Nuisance SpeciesSpecies Preserved Preserved

SpecimensSpecimens PhotographsPhotographs Dried SpecimensDried Specimens

Injurious WildlifeInjurious Wildlife Mounted SpecimensMounted Specimens

6.7. Port ID Reference 6.7. Port ID Reference CollectionCollection

Citrus Blackspot Citrus Blackspot DiseaseDisease

General Beauregard General Beauregard NewtonNewton

Unknown Specimens Unknown Specimens Collected on Beach in Dar Collected on Beach in Dar

es Salaam, TNZes Salaam, TNZ

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Recommendation: Put in Recommendation: Put in Quarantine and/or Fumigate Quarantine and/or Fumigate until Positively Identifieduntil Positively Identified

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