結 構 生 物 學 structural biology
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結 構 生 物 學 Structural Biology. 期 中 報 告. Speaker : Shiue-Tai Chen Adviser :鄒文雄 老師. Crystal Structure of Human Peroxiredoxin 5, a Novel Type of Mammalian Peroxiredoxin at 1.5 Å Resolution. PDB ID : 1HD2. - PowerPoint PPT PresentationTRANSCRIPT
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Speaker : Shiue-Tai ChenAdviser :鄒文雄 老師
結 構 生 物 學結 構 生 物 學Structural BiologyStructural Biology
期 中 報 告
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PDB ID: 1HD2
Crystal Structure of Human Peroxiredoxin 5, a Novel Type of Mammalian
Peroxiredoxin at 1.5 Å Resolution
Jean-Paul Declercq,Christine Evrard,Andre Clippe et al. Journal of Molecular Biology 311(4): 751-759 2001
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Reactive oxygen species (ROS) are constantly generated under normal conditions as a consequence of aerobic metabolism.
ROS include free radicals such as the superoxide anion (O2
.-), the hydroxyl radical (HO.), and th
e nonradical hydrogen peroxide (H2O2).
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To cope with the deleterious effects of reactive oxygen species, aerobic organisms are equipped with antioxidant enzymes.
More than a decade ago, a novel family of peroxidases named peroxiredoxins (Prxs) emerged. (Chae,etc.1994)
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Topological diagram showing the arrangement of the secondary structural elements in Peroxiredoxin 5
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Ribbon diagrams showing the overall organization of Peroxiredoxin 5.
(b) being a top view of (a).
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(a) Molecular surface coloured according to the local electrostatic potential,ranging from blue (the most positive region) to red (the most negative). (b) A diagram in which the side-chains of some important residues. (a) was produced using GRASP (b) using MOLSCRIPT26 and Raster3D.
View of the surrounding of the active-site pocket.
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View of the surrounding of the active-site pocket.
(c) Stereoscopic view oriented like (b) in which the electron density of a s-A28 map has been contoured at a level of 2 s around the side-chains
and the benzoate ion. (c) was produced using using O.
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What I will do?What I will do?
1. 使用 alignment in EBI and using GeneDoc in PSC
,為了比對 PeroxiredoxinⅠ~Ⅵ 胺基酸序列的特異性及保留性,與功能的關係。
2. 利用 Swiss pdb Viewer 軟體及網頁搜尋的PDB ID 檔,來觀察 PeroxiredoxinⅤ 與分子內或分子間的交互作用 (interaction) ,與酵素活性的關係。
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What I will do?What I will do?
3. 使用上述的方法,來討論我們實驗室利用不同的物種 cloning 出來的 Peroxiredoxin 與分子生物學研究結果的相關性。
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O2 O2-. SOD
H2O2
GPxH2O
ONOO-
NO.
NO2.
OH.
Fe2+
Fe3+
Catalase
PL
PLOOH
PLOH
Prx
Cu+
Cu2+
Prx
PHGPx
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Zachary A. Wood,etc.2003
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Zachary A. Wood,etc.2003
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K. Henkle-Du¨hrsen *, A. Kampko¨ tter,2001
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PDB IDPDB ID :: 1HD21HD2