应用 pcr 技术检测空气传播疾病
If you can't read please download the document
DESCRIPTION
应用 PCR 技术检测空气传播疾病. 乔杰 10808041. 目录. 背景 相关知识介绍 技术拓展 文献图例 举例：马铃薯晚疫病 讨论. 背景. - PowerPoint PPT PresentationTRANSCRIPT
-
PCR10808041
-
1845 184818491001002001845 US-11845 DNA
-
mechanism of transmissionroute of transmission mode of transmissionmode of spread
-
Bioaerosols() (15~35)
-
2x14x14230-25050110-115 6012
-
May Burkard Burkard 16.5 / 1 m1.5 ml (eppendorf vial)
-
Burkard 100(1)90Burkard102063-4(110240 )-1-9 0 Burkard 245m90%242/ 124/ 6 8/ 48
-
PCRPCRRT-PCRT-RFLPDGGE: DNA
-
T-RFLP16sRNA T-RFLP5HEX,TET,6-FAMDNAPCRPCRPCR4bp T-RFLP
-
T-RFLP
-
DNA : mRNA cDNA , cDNA DNA , , ,DNA
-
DGGEDNA50%TmTmDNAGCDGGE50~60 0~100%DNADNATmDNATmDNATmDGGEDNA10DGGEGCGCGC clamp530~40bpGCPCRDGGE100% DGGE1DGGE99%21kb100~500bp3DGGE4DGGE245GC DGGE
-
DGGE
-
DGGEPCRDNATmTm DNADNADNADGGEDNA
-
DNA1977SangerDNAMaxamGilbertDNA DNASangerATGCPAGEDNADNA
-
DNA()80DNA()1990ZagurskyMccornickcap()DNADNADNA()1980BainsDNADNA
-
()PCRPCRPCRDNA()cDNAcDNADNAcDNA
DNASoIexa()SoIexaSoIexaSoIexa
-
()Solexa()(1)DNADNA (2)DNADNA(3)(4)DNADNA(5)DNADNA (6)DNA(7)DNA (8)DNA(9)(10)
-
Pyrosequencing(Pyrosequencing)DNADNA Pyrosequencing4dNTPdNTPPPi PPiPyrogramTMdNTPDNA Pyrosequencing
-
11DNA(DNA PolymeraseATP SulfurylaseLuciferaseApyrase)(APSLuciferin) 21dNTPDNADNA 3(PPi)3ATPPPiAPSATPATPCCD4dNTPATPApyrase 5dNTP24DNA PyrosequecingSNP
-
Pyrosequencing
-
PCR
-
QoIscyt-bc1Qo cyt-bc1DNADNA
-
Potato Late Blight)20%-40%100
-
1. ,,,,,,,,,
-
2. Phytophthora infestans3-4
-
3. 6-12155cm
-
4. 90% ,,:(1)(2),
-
:,,,,,
-
5. (1)(2),,,,,(3),,,(4)40%200,58%64%50060%50072%70080072.2%8001212200,7101,231014
-
DNAPCRRNAPCRRNAPCR