動物科技系 營養生技實驗室
DESCRIPTION
動物科技系 營養生技實驗室. 研究室：生資大樓 302 室 教師辦公室：生資大樓 430 室 李德南. 動物營養學 營養與基因表現 試驗設計 豬學. 營養對動物免疫調控 靈芝對豬隻免疫功能之影響. 教授課程. 研究方向. 內容介紹. 消化道之生理重要性 營養對腸道生長和免疫發育之影響 科學研究發展展望 問題考核. 發炎性腸道疾病 inflammatory bowel disease (IBD). 慢性腸道發炎總稱 潰瘍性結腸炎 (Ulcerative colitis, UC) 克隆氏症 (Crohn’s disease, CD) - PowerPoint PPT PresentationTRANSCRIPT
-
302430
-
inflammatory bowel disease (IBD)
(Ulcerative colitis, UC)
(Crohns disease, CD)
7//
-
(UC)15-35
-
(Crohns disease, CD)
-
Innate immune responseMacrophageIL-1IL-6IL-8IL-12TTH1TNF-Dendritic cellIL-12Neutrophils
-
IBD ()TNF--InfliximabMesalamine (Asacol, 5-ASA)ProbioticSteroid
UC-DS-
-
* (a)17(b)24(Franklin et al., 2002)
Chart1
8.419.7569.81
8.957.476.079.87
8.168.315.799.25
7.469.446.129.9
7.488.876.379.68
7.28.85.789.94
E. coli
Lactobacill
Bifidobacteria
Total anaerobes
age (day)
cfu/g intestinal contents
17172024273134
E. coli8.418.958.167.467.487.2
Lactobacill9.757.478.319.448.878.8
Bifidobacteria66.075.796.126.375.78
Total anaerobes9.819.879.259.99.689.94
24172024273134
E. coli8.537.647.928.776.856.7
Lactobacill10.2310.9310.589.149.529.35
Bifidobacteria5.087.186.335.486.185.23
Total anaerobes9.8210.3610.0510.289.749.63
E. coli172024273134
178.418.958.167.467.487.2
248.537.647.928.776.856.7
Lactobacill172024273134
179.757.478.319.448.878.8
2410.2310.9310.589.149.529.35
E. coli
Lactobacill
Bifidobacteria
Total anaerobes
age (day)
cfu/g intestinal contents
17
E. coli
Lactobacill
Bifidobacteria
Total anaerobes
age(day)
cfu/g intestinal contents
24
Sheet3
17
24
age (day)
cfu/g intestinal contents
E. coli
17
24
age (day)
cfu/g intestinal contents
Lactobacill
17172024273134
E. coli8.849.258.777.37.577.23
Lactobacill8.678.157.858.688.378.13
Bifidobacteria5.325.675.886.516.546.83
Total anaerobes9.8610.219.689.219.058.6
24172024273134
E. coli8.578.688.88.57.738.02
Lactobacill8.369.68.768.347.688.2
Bifidobacteria4.495.655.255.927.136.03
Total anaerobes9.789.5510.3510.029.129.3
E. coli
Lactobacill
Bifidobacteria
Total anaerobes
age (day)
cfu/g fecal
17
E. coli
Lactobacill
Bifidobacteria
Total anaerobes
age (day)
cfu/g fecal
24
Chart2
8.5310.235.089.82
7.6410.937.1810.36
7.9210.586.3310.05
8.779.145.4810.28
6.859.526.189.74
6.79.355.239.63
E. coli
Lactobacill
Bifidobacteria
Total anaerobes
age (day)
cfu/g intestinal contents
17172024273134
E. coli8.418.958.167.467.487.2
Lactobacill9.757.478.319.448.878.8
Bifidobacteria66.075.796.126.375.78
Total anaerobes9.819.879.259.99.689.94
24172024273134
E. coli8.537.647.928.776.856.7
Lactobacill10.2310.9310.589.149.529.35
Bifidobacteria5.087.186.335.486.185.23
Total anaerobes9.8210.3610.0510.289.749.63
E. coli172024273134
178.418.958.167.467.487.2
248.537.647.928.776.856.7
Lactobacill172024273134
179.757.478.319.448.878.8
2410.2310.9310.589.149.529.35
E. coli
Lactobacill
Bifidobacteria
Total anaerobes
age (day)
cfu/g intestinal contents
17
E. coli
Lactobacill
Bifidobacteria
Total anaerobes
age(day)
cfu/g intestinal contents
24
Sheet3
17
24
age (day)
cfu/g intestinal contents
E. coli
17
24
age (day)
cfu/g intestinal contents
Lactobacill
17172024273134
E. coli8.849.258.777.37.577.23
Lactobacill8.678.157.858.688.378.13
Bifidobacteria5.325.675.886.516.546.83
Total anaerobes9.8610.219.689.219.058.6
24172024273134
E. coli8.578.688.88.57.738.02
Lactobacill8.369.68.768.347.688.2
Bifidobacteria4.495.655.255.927.136.03
Total anaerobes9.789.5510.3510.029.129.3
E. coli
Lactobacill
Bifidobacteria
Total anaerobes
age (day)
cfu/g fecal
17
E. coli
Lactobacill
Bifidobacteria
Total anaerobes
age (day)
cfu/g fecal
24
-
(A)(B)Montagne et al., 2007(C)(D)acccbaccbbaabbbabbbbabbbbabbbb
Chart4
597
356
359
377
457
Jejunum
Day post-weaning
(um)
Gu et al., 2002
182229364350
Duodenum18.613.215.615.417.820.3
Proximal jejunum19.813.015.115.317.920.2
Distal jejunum17.812.214.913.015.717.6
Ileum16.011.514.012.815.618.5
Duodenum3.31.01.52.11.60.9
Proximal jejunum2.51.32.01.50.80.4
Distal jejunum3.63.63.71.41.30.7
Ileum4.74.03.92.31.61.7
Gu et al., 2002
0000
0000
0000
0000
0000
0000
Duodenum
Proximal jejunum
Distal jejunum
Ileum
Age (days)
(IEL NO./100 villous epithelial cells)
Faure et al., 2005
0000
0000
0000
0000
0000
0000
Duodenum
Proximal jejunum
Distal jejunum
Ileum
Age (days)
(GC NO./100 villous epithelial cells)
Montagne et al., 2007
3060100150
Duodenum90140151120
Jejunum119130130153
Ileum120170165147
Colon8010011095
Montagne et al., 2007
10
7.5
15
7.5
Duodenum
Group
Protien FSR (%)
10
7.5
3.5
15
Duodenum
Group
Protien FSR (%)
7.5
15
7.5
7.5
Duodenum
Group
Protien FSR (%)
10
7.5
7.5
7.5
Duodenum
Group
Protien FSR (%)
Villous lenth (m)025815
Jejunum597356359377457
Crypt depth (m)
Jejunum216211367304308
Lactase (IU/mg protein)
Jejunum5948171712
Ileum11.43.91.73.31.4
APN (IU/mg protein)
Jejunum5126283131
Ileum4821243127
Jejunum
Day post-weaning
(um)
0
2
5
8
15
(IU/mg protein)
0
2
5
8
15
(IU/mg protein)
Jejunum
Day post-weaning
(um)
Chart5
5948171712
11.43.91.73.31.4
0
2
5
8
15
(IU/mg protein)
Gu et al., 2002
182229364350
Duodenum18.613.215.615.417.820.3
Proximal jejunum19.813.015.115.317.920.2
Distal jejunum17.812.214.913.015.717.6
Ileum16.011.514.012.815.618.5
Duodenum3.31.01.52.11.60.9
Proximal jejunum2.51.32.01.50.80.4
Distal jejunum3.63.63.71.41.30.7
Ileum4.74.03.92.31.61.7
Gu et al., 2002
0000
0000
0000
0000
0000
0000
Duodenum
Proximal jejunum
Distal jejunum
Ileum
Age (days)
(IEL NO./100 villous epithelial cells)
Faure et al., 2005
0000
0000
0000
0000
0000
0000
Duodenum
Proximal jejunum
Distal jejunum
Ileum
Age (days)
(GC NO./100 villous epithelial cells)
Montagne et al., 2007
3060100150
Duodenum90140151120
Jejunum119130130153
Ileum120170165147
Colon8010011095
Montagne et al., 2007
10
7.5
15
7.5
Duodenum
Group
Protien FSR (%)
10
7.5
3.5
15
Duodenum
Group
Protien FSR (%)
7.5
15
7.5
7.5
Duodenum
Group
Protien FSR (%)
10
7.5
7.5
7.5
Duodenum
Group
Protien FSR (%)
Villous lenth (m)025815
Jejunum597356359377457
Crypt depth (m)
Jejunum216211367304308
Lactase (IU/mg protein)
Jejunum5948171712
Ileum11.43.91.73.31.4
APN (IU/mg protein)
Jejunum5126283131
Ileum4821243127
Jejunum
Day post-weaning
(um)
0
2
5
8
15
(IU/mg protein)
0
2
5
8
15
(IU/mg protein)
Jejunum
Day post-weaning
(um)
Chart6
5126283131
4821243127
0
2
5
8
15
(IU/mg protein)
Gu et al., 2002
182229364350
Duodenum18.613.215.615.417.820.3
Proximal jejunum19.813.015.115.317.920.2
Distal jejunum17.812.214.913.015.717.6
Ileum16.011.514.012.815.618.5
Duodenum3.31.01.52.11.60.9
Proximal jejunum2.51.32.01.50.80.4
Distal jejunum3.63.63.71.41.30.7
Ileum4.74.03.92.31.61.7
Gu et al., 2002
0000
0000
0000
0000
0000
0000
Duodenum
Proximal jejunum
Distal jejunum
Ileum
Age (days)
(IEL NO./100 villous epithelial cells)
Faure et al., 2005
0000
0000
0000
0000
0000
0000
Duodenum
Proximal jejunum
Distal jejunum
Ileum
Age (days)
(GC NO./100 villous epithelial cells)
Montagne et al., 2007
3060100150
Duodenum90140151120
Jejunum119130130153
Ileum120170165147
Colon8010011095
Montagne et al., 2007
10
7.5
15
7.5
Duodenum
Group
Protien FSR (%)
10
7.5
3.5
15
Duodenum
Group
Protien FSR (%)
7.5
15
7.5
7.5
Duodenum
Group
Protien FSR (%)
10
7.5
7.5
7.5
Duodenum
Group
Protien FSR (%)
Villous lenth (m)025815
Jejunum597356359377457
Crypt depth (m)
Jejunum216211367304308
Lactase (IU/mg protein)
Jejunum5948171712
Ileum11.43.91.73.31.4
APN (IU/mg protein)
Jejunum5126283131
Ileum4821243127
Jejunum
Day post-weaning
(um)
0
2
5
8
15
(IU/mg protein)
0
2
5
8
15
(IU/mg protein)
Jejunum
Day post-weaning
(um)
Chart7
216
211
367
304
308
Jejunum
Day post-weaning
(um)
Gu et al., 2002
182229364350
Duodenum18.613.215.615.417.820.3
Proximal jejunum19.813.015.115.317.920.2
Distal jejunum17.812.214.913.015.717.6
Ileum16.011.514.012.815.618.5
Duodenum3.31.01.52.11.60.9
Proximal jejunum2.51.32.01.50.80.4
Distal jejunum3.63.63.71.41.30.7
Ileum4.74.03.92.31.61.7
Gu et al., 2002
0000
0000
0000
0000
0000
0000
Duodenum
Proximal jejunum
Distal jejunum
Ileum
Age (days)
(IEL NO./100 villous epithelial cells)
Faure et al., 2005
0000
0000
0000
0000
0000
0000
Duodenum
Proximal jejunum
Distal jejunum
Ileum
Age (days)
(GC NO./100 villous epithelial cells)
Montagne et al., 2007
3060100150
Duodenum90140151120
Jejunum119130130153
Ileum120170165147
Colon8010011095
Montagne et al., 2007
10
7.5
15
7.5
Duodenum
Group
Protien FSR (%)
10
7.5
3.5
15
Duodenum
Group
Protien FSR (%)
7.5
15
7.5
7.5
Duodenum
Group
Protien FSR (%)
10
7.5
7.5
7.5
Duodenum
Group
Protien FSR (%)
Villous lenth (m)025815
Jejunum597356359377457
Crypt depth (m)
Jejunum216211367304308
Lactase (IU/mg protein)
Jejunum5948171712
Ileum11.43.91.73.31.4
APN (IU/mg protein)
Jejunum5126283131
Ileum4821243127
Jejunum
Day post-weaning
(um)
0
2
5
8
15
(IU/mg protein)
0
2
5
8
15
(IU/mg protein)
Jejunum
Day post-weaning
(um)
-
IBD
-
?
-
-
3.
-
()
(MHC)
-
T B ()
-
5. (A)(B)(C)M (IEL)(ILP) (PP) (MLN)(Spleen)
-
-
70%
-
FIG. A schematic representation of the temporal nature of the host immune response to invasion by a pathogenic microorganism.
-
(Klasing, 1998)
-
2. G27S93Lane 1MKLane 2hEGFLane 3GS115Lane 4G270 hLane 5G2772 hLane 6SMD1168Lane 7S930 hLane 8S9372 h6 kDa(EGF)
-
3. (MeOH=0.028 h-1)
-
(4) pEGF 1.5 mg/kg 143214(1)
-
acDNAEGF 0 0.5 1.0 1.5
Fig. 8. Effects of diets supplemented with recombinant pEGF on jejunal maltase in early-weaned piglets * *(c) ActivityGAPDHMaltase
Chart1
0.10.09
0.170.13
0.540.24
0.390.27
pEGF (mg/kg)
Relative mRNA (%)
bExpression
Sheet1
Jejunum
00.1
0.50.17
10.54
1.50.39
Ileum
00.12
0.50.17
10.19
1.50.15
Jejunum
00.46
0.50.9
10.97
1.51.28
Ileum
00.08
0.50.35
10.19
1.50.22
Sheet1
00.09
00.13
00.24
00.27
EGF (mg/kg)
Relative mRNA (%)
(B) Maltase
Sheet2
00.09
00.19
00.16
00.17
EGF (mg/kg)
Relative mRNA (%)
(B) Maltase
Sheet3
00.32
00.23
00.39
00.27
EGF (mg/kg)
Relative mRNA (%)
(B) Lactase
00.12
00.42
00.23
00.16
EGF (mg/kg)
Relative mRNA (%)
(B) Lactase
Chart6
87.5921.7
132.725.02
10616.92
164.929.81
Maltase
pEGF (mg/kg)
Activity (mol/mg protein/min)
growth
pEGFDaily gain
0186.4
0.5186.8
1142.7
1.5204.9
Feed intake
0191.1
0.5231
1206.9
1.5216.2
F: G
00.88
0.50.8
10.69
1.50.97
growth
186.471.11
186.832.4
142.734.85
204.918.43
Daily gain
pEGF (mg/kg)
Daily gain (g/day)
DNA
191.145.54
23118.32
206.938.19
216.229.24
Feed intake
pEGF (mg/kg)
Feed intake (g/day)
Enzyme
0.880.18
0.80.12
0.690.12
0.970.1
F: G
pEGF (mg/kg)
F: G
Pepsin
Mucosa wt
034.42
0.561.32
144.35
1.578.32
DNA
01.52
0.51.4
11.51
1.51.46
Protein
089.16
0.594.02
194.99
1.5103.4
Pepsin
9.64
11.44
8.12
19.05
Mucosa wt
pEGF (mg/kg)
Weight (mg/cm)
villus
0.21
0.25
0.27
0.26
DNA
pEGF (mg/kg)
DNA (mg/g mucosa)
3.15
4.96
2.71
2.75
Protein
pEGF (mg/kg)
Protein (mg/g mucosa)
Maltase
087.59
0.5132.7
1106
1.5164.9
21.7
25.02
16.92
29.81
Maltase
pEGF (mg/kg)
Activity (mol/mg protein/min)
Pepsin
0113.6
0.5203.1
1158.1
1.5139.4
22.42
30.16
18.23
12.16
Pepsin
pEGF (mg/kg)
Pepsin activity (mol/mg protein/min)
Villus height
0249.5
0.5329.2
1300.6
1.5361.3
Crypt depth
0202.9
0.5221.9
1206.4
1.5191.5
Villus height/Crypt depth
01.3
0.51.62
11.52
1.51.91
16.01
35.04
14.85
44.17
Villus height
pEGF (mg/kg)
Height (m)
18.53
29.29
18.47
9.62
Crypt depth
pEGF (mg/kg)
Depth (m)
0.21
0.31
0.13
0.24
Villus height/Crypt depth
pEGF (mg/kg)
Villus height/crypt depth
-
0.6% OA ()0.1% NT ()0.6%0.1%
-
4. PWMa) 28(b) 46 * * *64%10%79%
Chart5
93.8415.91
91.9214.67
10622.2
144.426.03
PWM
Treatment
Stimulated index
Sheet1
Daily gain
Control568
NT566
OA569
NT+OA562
Sheet1
011.56
015.54
013.62
012.08
Daily gain
Treatment
Daily gain (g/day)
Sheet2
Feed concumption
Control959
NT937
OA952
NT+OA909
Sheet2
026.69
017.37
021.55
012.78
Feed concumption
Treatment
Feed concumption (g/day)
Sheet3
Feed/gain
Control1.28
NT1.28
OA1.23
NT+OA1.23
Sheet3
00.18
00.2
00.15
00.23
Feed/gain
Treatment
Feed/gain
Sheet4
d0-d28Diarrhea
Control2.41
NT0.45
OA2.14
NT+OA0.18
Sheet4
02.13
00.45
02.14
00.09
Diarrhea
Treatment
Diarrhea (%)
Sheet5
PWMpbmc d 28
Control93.84
NT91.92
OA106
NT+OA144.4
Sheet5
015.91
014.67
022.2
026.03
PWM
Treatment
Stimulated index
Sheet6
PWMpbmc d 46
Control125.4
NT203.6
OA224
NT+OA221.8
Sheet6
022.49
027.34
038.97
041.57
PWM
Treatment
Stimulated index
Sheet7
Plasma d28IgA
Control0.27
NT0.31
OA0.31
NT+OA0.38
Plasma d46IgA
Control0.33
NT0.35
OA0.37
NT+OA0.41
Sheet7
00.02
0
0
0
IgA
Treatment
IgA (mg/ml)
Sheet8
00.03
0
0
0
IgA
Treatment
IgA (mg/ml)
d28Ca
Control2.76
NT2.69
OA2.69
NT+OA2.66
d46Ca
Control2.65
NT2.64
OA2.54
NT+OA2.62
00.03
0
0
0
Ca
Treatment
Ca (mmol/l)
00.05
0
0
0
Ca
Treatment
Ca (mmol/l)
Chart6
125.422.49
203.627.34
22438.97
221.841.57
PWM
Treatment
Stimulated index
Sheet1
Daily gain
Control568
NT566
OA569
NT+OA562
Sheet1
011.56
015.54
013.62
012.08
Daily gain
Treatment
Daily gain (g/day)
Sheet2
Feed concumption
Control959
NT937
OA952
NT+OA909
Sheet2
026.69
017.37
021.55
012.78
Feed concumption
Treatment
Feed concumption (g/day)
Sheet3
Feed/gain
Control1.28
NT1.28
OA1.23
NT+OA1.23
Sheet3
00.18
00.2
00.15
00.23
Feed/gain
Treatment
Feed/gain
Sheet4
d0-d28Diarrhea
Control2.41
NT0.45
OA2.14
NT+OA0.18
Sheet4
02.13
00.45
02.14
00.09
Diarrhea
Treatment
Diarrhea (%)
Sheet5
PWMpbmc d 28
Control93.84
NT91.92
OA106
NT+OA144.4
Sheet5
015.91
014.67
022.2
026.03
PWM
Treatment
Stimulated index
Sheet6
PWMpbmc d 46
Control125.4
NT203.6
OA224
NT+OA221.8
Sheet6
022.49
027.34
038.97
041.57
PWM
Treatment
Stimulated index
Sheet7
Plasma d28IgA
Control0.27
NT0.31
OA0.31
NT+OA0.38
Plasma d46IgA
Control0.33
NT0.35
OA0.37
NT+OA0.41
Sheet7
00.02
0
0
0
IgA
Treatment
IgA (mg/ml)
Sheet8
00.03
0
0
0
IgA
Treatment
IgA (mg/ml)
d28Ca
Control2.76
NT2.69
OA2.69
NT+OA2.66
d46Ca
Control2.65
NT2.64
OA2.54
NT+OA2.62
00.03
0
0
0
Ca
Treatment
Ca (mmol/l)
00.05
0
0
0
Ca
Treatment
Ca (mmol/l)
-
(resistant starch, RS)
1992
-
-
Wheat starchItemCornDriedSteamedAutoclaved-cooledTotal starch (%)58.8492.5287.7478.07Resistant starch (%)3.250.053.397.58
- 11. 35TP
-
12. IgA(n=16)
-
*1-7 (a) Rep 1(b) Rep 2(c) overallp0.1
-
*2-7 WST effect, p0.01WST vs AGP, p0.05
-
*2-8 abbbbPro effect, p0.05
-
40%/
Chart1
0.951.31.141.220.070.240.10.17
1.191.150.981.240.01000
0% NaB
0.15% NaB
0.30% NaB
0.45% NaB
Lactobacillus/E. coli
Na-ButyrateJejunumIleum
0% NaB179.7114.4
0.15% NaB174100
0.30% NaB197.2108.9
0.45% NaB204.6119.3
000032.4220.219.9612.57
000022.4520.276.249.41
0% NaB
0.15% NaB
0.30% NaB
0.45% NaB
(ug/g digesta)
Na-ButyrateIleumCecum
0% NaB0.951.19
0.15% NaB1.31.15
0.30% NaB1.140.98
0.45% NaB1.221.24
00000.070.240.10.17
00000.01000
0% NaB
0.15% NaB
0.30% NaB
0.45% NaB
Lactobacillus/E. coli
IgA
Na-ButyratePlasmaBileJejunumIleum
03.2978.575.89.3
0.152.561058.5910.16
0.34.4994.839.1510.82
0.454.4195.9310.117.01
Na-ButyratePBMCSpleenPeyer's patchMLN
0% NaB63.1535.5337.46131.6
0.15% NaB128.531.3160.03138.4
0.30% NaB45.7220.0123.7260.36
0.45% NaB62.814.2839.42137.2
000019.9319.419.2126.84
0% NaB
0.15% NaB
0.30% NaB
0.45% NaB
Stimulated index
IFN-r
00009.4317.253.745
0% NaB
0.15% NaB
0.30% NaB
0.45% NaB
Stimulated index
00006.9224.964.8110.56
0% NaB
0.15% NaB
0.30% NaB
0.45% NaB
Stimulated index
000039.6433.0817.488.61
0% NaB
0.15% NaB
0.30% NaB
0.45% NaB
Stimulated index
Na-ButyratePBMCSpleenPeyer's patchMLN
0% NaB1111
0.15% NaB2.581.731.56.24
0.30% NaB1.562.411.154.45
0.45% NaB4.871.841.954.65
00000.40.980.71.12
0% NaB
0.15% NaB
0.30% NaB
0.45% NaB
Secreted fold
00000.590.491.931.77
0% NaB
0.15% NaB
0.30% NaB
0.45% NaB
Secreted fold
00000.010.040.080.08
0% NaB
0.15% NaB
0.30% NaB
0.45% NaB
Secreted fold
00000.480.540.550.54
0% NaB
0.15% NaB
0.30% NaB
0.45% NaB
Secreted fold
-
() : 0-140.09% 15-42 0.06 % (Thr) 0.1% (NaB+Thr)
- 19.NaB effect P
- 20.NaB effect P
-
(GLF)Ganoderma lucidum BCRC 36123
-
-
GLF--K-YBGL, Megazyme
Fang2002GLF-
- = -
-
1 ItemGLFSubstrate-glucan (%)59.68 0.5157.20 0.77
-glucan (%) 3.76 0.18 0.57 0.06
Total-glucan (%)63.45 0.3358.55 0.83
Ganoderic acid (mg/g)0.06 0.016ND
The data were shown at the mean SE (n=4). ND = Not detected.GLF*
-
()320
4 20 60 ppm107 ppm322 ppm225 ppm
-
6.
Treatments Polysaccharide, ppm
0107225322SEM p-value
Basic (M)1.25a1.18a1.01a1.74b0.130.003
Con A (M)1.60a2.03a1.58a3.17b0.290.002
-
*1.1 Repeated Measurement
ADGP-valueTRT0.023TIME0.001TRT*TIME0.980
-
*1.2 4.7%abbb
-
*1.3 baaaRepeated Measurement
ADGP-valueTRT0.002TIME0.001TRT*TIME0.009
-
DPPH> 100 umol trolox /gTEAC > 100~200 umol trolox /g> 10~30 mg/g> 50%Amylase> 400~1200 U/g (per min)
-
abmg/kgbbbnmol/mg, proteinmg/kgbacdd1 (A) (B)(MDA)
Chart4
0.610.006
0.46
0.42
0.41
0.41
MDA
Sheet1
TEAC-PlasmaSEMDA
04.260.0016100.61
6004.290.0017336000.46
12004.350.0016612000.42
18004.40.00180818000.41
Vit E 1004.440.001604Vit E 1000.41
Sheet1
0
0
0
0
0
TEAC-Plasma
TEAC(mM)
Sheet2
00.006
0
0
0
0
MDA
MDA
Sheet3
Chart5
4.260.0016
4.29
4.35
4.4
4.44
TEAC-Plasma
TEAC(mM)
Sheet1
TEAC-PlasmaSEMDA
04.260.0016100.61
6004.290.0017336000.46
12004.350.0016612000.42
18004.40.00180818000.41
Vit E 1004.440.001604Vit E 1000.41
Sheet1
00.0016
0
0
0
0
TEAC-Plasma
TEAC(mM)
TEAC-Plasma
Sheet2
00.006
0
0
0
0
MDA
MDA
Sheet3
-
2 (A) CuZn-SODmg/kgbababaamg/kgabbbb(B)
Chart6
443.2716.59125
720.17
598.01
586.43
637.23
CuZn-SOD
CuZn-SOD (U/g, hemoglobin)
Sheet1
TEAC-PlasmaSEMDACuZn-SOD(U/g, hemoglobin)
04.260.0016100.610443.27
6004.290.0017336000.46600720.17
12004.350.0016612000.421200598.01
18004.40.00180818000.411800586.43
Vit E 1004.440.001604Vit E 1000.41Vit E 100637.23
Sheet1
00.0016
0
0
0
0
TEAC-Plasma
TEAC(mM)
TEAC-Plasma
Sheet2
00.006
0
0
0
0
MDA
MDA
Sheet3
016.59125
0
0
0
0
CuZn-SOD
(U/g, hemoglobin)
CuZn-SOD
Chart9
1.740.01437
2.07
1.95
1.91
2.06
CAT
Catalase (U/g, hemoglobin)
Sheet1
TEAC-PlasmaSEMDACuZn-SODCAT
04.260.0016100.610443.2701.74
6004.290.0017336000.46600720.176002.07
12004.350.0016612000.421200598.0112001.95
18004.40.00180818000.411800586.4318001.91
Vit E 1004.440.001604Vit E 1000.41Vit E 100637.23Vit E 1002.06
0.01437
Sheet1
00.0016
0
0
0
0
TEAC-Plasma
TEAC(mM)
TEAC-Plasma
Sheet2
0.610.006
0.46
0.42
0.41
0.41
MDA
MDA
Sheet3
016.59125
0
0
0
0
CuZn-SOD
(U/g, hemoglobin)
CuZn-SOD
00.01437
0
0
0
0
CAT
(U/g, hemoglobin)
-
3 mg/kg(A) aababbbmg/kg(B) aabacbc
Chart10
11.470.18887
9.66
8.49
10.49
8.79
Haptoglobin
Haptoglobin (mgHgb/mL)
Sheet1
TEAC-PlasmaSEMDACuZn-SODCATHaptoglobin
04.260.0016100.610443.2701.74011.47
6004.290.0017336000.46600720.176002.076009.66
12004.350.0016612000.421200598.0112001.9512008.49
18004.40.00180818000.411800586.4318001.91180010.49
Vit E 1004.440.001604Vit E 1000.41Vit E 100637.23Vit E 1002.06Vit E 1008.79
0.18887
0.01437
Sheet1
00.0016
0
0
0
0
TEAC-Plasma
TEAC(mM)
TEAC-Plasma
Sheet2
00.006
0
0
0
0
MDA
MDA
Sheet3
016.59125
0
0
0
0
CuZn-SOD
(U/g, hemoglobin)
CuZn-SOD
00.01437
0
0
0
0
CAT
(U/g, hemoglobin)
00.18887
0
0
0
0
Haptoglobin
(mgHgb/mL)
Chart11
44.90.39139
45.81
41.07
40.07
36.42
Lysozyme
Lysozyme (U/mL)
Sheet1
TEAC-PlasmaSEMDACuZn-SODCATHaptoglobinLysozyme
04.260.0016100.610443.2701.74011.47044.9
6004.290.0017336000.46600720.176002.076009.6660045.81
12004.350.0016612000.421200598.0112001.9512008.49120041.07
18004.40.00180818000.411800586.4318001.91180010.49180040.07
Vit E 1004.440.001604Vit E 1000.41Vit E 100637.23Vit E 1002.06Vit E 1008.79Vit E 10036.42
0.188870.39139
0.01437
Sheet1
4.260.0016
4.29
4.35
4.4
4.44
TEAC-Plasma
TEAC(mM)
TEAC-Plasma
Sheet2
0.610.006
0.46
0.42
0.41
0.41
MDA
MDA
Sheet3
443.2716.59125
720.17
598.01
586.43
637.23
CuZn-SOD
(U/g, hemoglobin)
CuZn-SOD
00.01437
0
0
0
0
CAT
(U/g, hemoglobin)
00.18887
0
0
0
0
Haptoglobin
(mgHgb/mL)
00.39139
0
0
0
0
Lysozyme
(U/mL)
-
2
-
()()()
-
: 40%: 60%1224
-
**
3-5
*****7.81 30.55**