Medical Immunology

(Immunoglobulin)

(Immunoglobulin , Ig)

Antigen +-2Albuminglobulin1IgGIgAIgMIgD

(antibodyAb): B: (Ig)

(Secreted Ig , SIg) (Membrane Ig , MIg)B

Y(heavy chain, H)(light chain, L)

A four-chain unit of Ig

1. heavy chain, H50~75kD 450~550 Aa. HCIg IgMIgDIgEIgAIgG IgG1-G4IgA1-A22. light chain, L 25kD 214Aa. Ig (type)1234

1. variable region, V 110AaIg N 1/2 1/41/5 Aa2.constant region, C IgC1/23/44/5 Aa3.hypervariable region, HVR VVHVL3AbAgcomplementarity determining region, CDRCDR1CDR2CDR34.framework region, FR CDR

VC CDRFR

hinge region CH1CH2 YIgDIgGIgAIgMIgE

domain

domain2.Ig immunoglobulin superfamily,IgSF

domain3.:(1)VHVL (2)CH13CL Ig(3)CH2(IgG)CH3(IgM) C1q (4)CH2CH3(IgG) (5)CH3(IgG) FcR(6)CH4(IgE) FcR

Ig

IgJ

Ig

FabFcPapain

pepsinF(ab`)2pFc`

1.Ig, 2.Ig(IgG)

Ig subtype

Ig Ig Ig

isotypeIg CallotypeIg C idiotypeIgId) HVR/CDR/IdIg Ig

IgV

IgC IgMIgG1-3 IgAIgEIgG4

Fc IgG + () FcR

ADCC) IgG + () Fc RNK

IgEIgC

IgGIgMIgAIgDIgE

IgG: IgG1 IgG2 IgG3 IgG4 2023 23W

IgM1.(900 kd) 2.Ig3. Ig 4.Ig510% 5.5 IgM 6.7.IgM IgM 8.IgMBCRB9.

IgA1.

2.63.Ig515%4.5.IgA IgA1

IgA2,

IgD1.2. B, 3. 1B 24.Ig1%5. 3

IgE1.2.(Ig0.002%)3.3 4.5.6.IgE

pollyclonal antibodypAb clone

monoclonal antibodyMcAb) B

(genetic engineering antibody)1. DNAIg

()VC Ig IgAbHVRIgGIgM IgDIgAIgEMcAb

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